Experiment: sodium carbonate 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1559 and Pf6N2E2_1560 are separated by 174 nucleotides Pf6N2E2_1560 and Pf6N2E2_1561 are separated by 113 nucleotides Pf6N2E2_1561 and Pf6N2E2_1562 are separated by 154 nucleotides
Pf6N2E2_1559 - Methyl-accepting chemotaxis protein, at 1,820,418 to 1,821,683
_1559
Pf6N2E2_1560 - Glycine cleavage system transcriptional activator GcvA, at 1,821,858 to 1,822,763
_1560
Pf6N2E2_1561 - Glutathione S-transferase (EC 2.5.1.18), at 1,822,877 to 1,823,476
_1561
Pf6N2E2_1562 - Threonine dehydratase (EC 4.3.1.19), at 1,823,631 to 1,824,587
_1562
Position (kb)
1821
1822
1823 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1820.954 kb on + strand, within Pf6N2E2_1559 at 1820.955 kb on - strand, within Pf6N2E2_1559 at 1820.955 kb on - strand, within Pf6N2E2_1559 at 1821.062 kb on - strand, within Pf6N2E2_1559 at 1821.062 kb on - strand, within Pf6N2E2_1559 at 1821.073 kb on + strand, within Pf6N2E2_1559 at 1821.172 kb on + strand, within Pf6N2E2_1559 at 1821.244 kb on + strand, within Pf6N2E2_1559 at 1821.244 kb on + strand, within Pf6N2E2_1559 at 1821.391 kb on + strand, within Pf6N2E2_1559 at 1821.391 kb on + strand, within Pf6N2E2_1559 at 1821.391 kb on + strand, within Pf6N2E2_1559 at 1821.392 kb on - strand, within Pf6N2E2_1559 at 1821.437 kb on + strand, within Pf6N2E2_1559 at 1821.439 kb on + strand, within Pf6N2E2_1559 at 1821.439 kb on + strand, within Pf6N2E2_1559 at 1821.439 kb on + strand, within Pf6N2E2_1559 at 1821.439 kb on + strand, within Pf6N2E2_1559 at 1821.439 kb on + strand, within Pf6N2E2_1559 at 1821.440 kb on - strand, within Pf6N2E2_1559 at 1821.703 kb on - strand at 1821.715 kb on + strand at 1821.716 kb on - strand at 1821.716 kb on - strand at 1821.716 kb on - strand at 1821.716 kb on - strand at 1821.716 kb on - strand at 1821.716 kb on - strand at 1821.716 kb on - strand at 1821.716 kb on - strand at 1821.716 kb on - strand at 1821.764 kb on + strand at 1821.764 kb on + strand at 1821.765 kb on - strand at 1821.778 kb on + strand at 1821.779 kb on - strand at 1821.813 kb on + strand at 1821.813 kb on + strand at 1821.813 kb on + strand at 1821.813 kb on + strand at 1821.813 kb on + strand at 1821.813 kb on + strand at 1821.814 kb on - strand at 1821.814 kb on - strand at 1821.814 kb on - strand at 1821.903 kb on - strand at 1821.903 kb on - strand at 1822.028 kb on - strand, within Pf6N2E2_1560 at 1822.373 kb on + strand, within Pf6N2E2_1560 at 1822.373 kb on + strand, within Pf6N2E2_1560 at 1822.558 kb on + strand, within Pf6N2E2_1560 at 1822.558 kb on + strand, within Pf6N2E2_1560 at 1822.703 kb on + strand at 1822.730 kb on + strand at 1822.795 kb on - strand at 1822.799 kb on + strand at 1822.799 kb on + strand at 1822.886 kb on + strand at 1822.887 kb on - strand at 1822.887 kb on - strand at 1823.027 kb on + strand, within Pf6N2E2_1561 at 1823.027 kb on + strand, within Pf6N2E2_1561 at 1823.027 kb on + strand, within Pf6N2E2_1561 at 1823.090 kb on + strand, within Pf6N2E2_1561 at 1823.179 kb on + strand, within Pf6N2E2_1561 at 1823.180 kb on - strand, within Pf6N2E2_1561 at 1823.225 kb on - strand, within Pf6N2E2_1561 at 1823.279 kb on + strand, within Pf6N2E2_1561 at 1823.279 kb on + strand, within Pf6N2E2_1561 at 1823.280 kb on - strand, within Pf6N2E2_1561 at 1823.342 kb on + strand, within Pf6N2E2_1561 at 1823.342 kb on + strand, within Pf6N2E2_1561 at 1823.342 kb on + strand, within Pf6N2E2_1561 at 1823.342 kb on + strand, within Pf6N2E2_1561 at 1823.343 kb on - strand, within Pf6N2E2_1561 at 1823.343 kb on - strand, within Pf6N2E2_1561 at 1823.343 kb on - strand, within Pf6N2E2_1561 at 1823.343 kb on - strand, within Pf6N2E2_1561 at 1823.475 kb on - strand at 1823.650 kb on + strand at 1823.651 kb on - strand at 1823.651 kb on - strand at 1823.666 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction sodium carbonate 0.0125 M remove 1,820,954 + Pf6N2E2_1559 0.42 -0.9 1,820,955 - Pf6N2E2_1559 0.42 -0.1 1,820,955 - Pf6N2E2_1559 0.42 -0.1 1,821,062 - Pf6N2E2_1559 0.51 +1.1 1,821,062 - Pf6N2E2_1559 0.51 +1.0 1,821,073 + Pf6N2E2_1559 0.52 +1.6 1,821,172 + Pf6N2E2_1559 0.60 -0.3 1,821,244 + Pf6N2E2_1559 0.65 -0.3 1,821,244 + Pf6N2E2_1559 0.65 +0.1 1,821,391 + Pf6N2E2_1559 0.77 +0.7 1,821,391 + Pf6N2E2_1559 0.77 -0.5 1,821,391 + Pf6N2E2_1559 0.77 +1.0 1,821,392 - Pf6N2E2_1559 0.77 +0.3 1,821,437 + Pf6N2E2_1559 0.80 +0.3 1,821,439 + Pf6N2E2_1559 0.81 +0.5 1,821,439 + Pf6N2E2_1559 0.81 -1.4 1,821,439 + Pf6N2E2_1559 0.81 -0.1 1,821,439 + Pf6N2E2_1559 0.81 +1.4 1,821,439 + Pf6N2E2_1559 0.81 +0.5 1,821,440 - Pf6N2E2_1559 0.81 +0.4 1,821,703 - +1.4 1,821,715 + -1.8 1,821,716 - +1.1 1,821,716 - +0.1 1,821,716 - -0.0 1,821,716 - -0.3 1,821,716 - -0.0 1,821,716 - +0.1 1,821,716 - +0.0 1,821,716 - -0.5 1,821,716 - +0.1 1,821,764 + -1.9 1,821,764 + +0.7 1,821,765 - +1.8 1,821,778 + -0.1 1,821,779 - +0.3 1,821,813 + -0.1 1,821,813 + -1.3 1,821,813 + -0.9 1,821,813 + +0.9 1,821,813 + +0.9 1,821,813 + +0.1 1,821,814 - +1.1 1,821,814 - +0.1 1,821,814 - -0.1 1,821,903 - -0.3 1,821,903 - +0.3 1,822,028 - Pf6N2E2_1560 0.19 -0.5 1,822,373 + Pf6N2E2_1560 0.57 -1.5 1,822,373 + Pf6N2E2_1560 0.57 -1.1 1,822,558 + Pf6N2E2_1560 0.77 -0.2 1,822,558 + Pf6N2E2_1560 0.77 -0.8 1,822,703 + +0.3 1,822,730 + -0.5 1,822,795 - -0.5 1,822,799 + +0.0 1,822,799 + +0.1 1,822,886 + +0.1 1,822,887 - -0.0 1,822,887 - -0.1 1,823,027 + Pf6N2E2_1561 0.25 +0.6 1,823,027 + Pf6N2E2_1561 0.25 -1.9 1,823,027 + Pf6N2E2_1561 0.25 +1.1 1,823,090 + Pf6N2E2_1561 0.35 -0.4 1,823,179 + Pf6N2E2_1561 0.50 +0.2 1,823,180 - Pf6N2E2_1561 0.51 +1.1 1,823,225 - Pf6N2E2_1561 0.58 -0.4 1,823,279 + Pf6N2E2_1561 0.67 -1.2 1,823,279 + Pf6N2E2_1561 0.67 -0.9 1,823,280 - Pf6N2E2_1561 0.67 +0.3 1,823,342 + Pf6N2E2_1561 0.78 +0.2 1,823,342 + Pf6N2E2_1561 0.78 -0.9 1,823,342 + Pf6N2E2_1561 0.78 +1.1 1,823,342 + Pf6N2E2_1561 0.78 -0.7 1,823,343 - Pf6N2E2_1561 0.78 +0.3 1,823,343 - Pf6N2E2_1561 0.78 -0.1 1,823,343 - Pf6N2E2_1561 0.78 +2.1 1,823,343 - Pf6N2E2_1561 0.78 -0.5 1,823,475 - -0.3 1,823,650 + +0.2 1,823,651 - +1.7 1,823,651 - -0.0 1,823,666 + +0.3
Or see this region's nucleotide sequence