Experiment: LB 37C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5978 and Pf1N1B4_5979 are separated by 120 nucleotides Pf1N1B4_5979 and Pf1N1B4_5980 are separated by 255 nucleotides Pf1N1B4_5980 and Pf1N1B4_5981 are separated by 239 nucleotides
Pf1N1B4_5978 - Ketoglutarate semialdehyde dehydrogenase (EC 1.2.1.26), at 426,416 to 427,996
_5978
Pf1N1B4_5979 - Ureidoglycolate/malate/sulfolactate dehydrogenase family (EC 1.1.1.-), at 428,117 to 429,124
_5979
Pf1N1B4_5980 - Organic hydroperoxide resistance protein, at 429,380 to 429,832
_5980
Pf1N1B4_5981 - Organic hydroperoxide resistance transcriptional regulator, at 430,072 to 430,533
_5981
Position (kb) 428
429
430 Strain fitness (log2 ratio) -2
-1
0
1 at 427.141 kb on - strand, within Pf1N1B4_5978 at 427.442 kb on + strand, within Pf1N1B4_5978 at 427.483 kb on - strand, within Pf1N1B4_5978 at 428.145 kb on + strand at 428.146 kb on - strand at 428.146 kb on - strand at 428.361 kb on + strand, within Pf1N1B4_5979 at 428.362 kb on - strand, within Pf1N1B4_5979 at 428.362 kb on - strand, within Pf1N1B4_5979 at 428.711 kb on - strand, within Pf1N1B4_5979 at 428.711 kb on - strand, within Pf1N1B4_5979 at 429.214 kb on + strand at 429.236 kb on - strand at 429.684 kb on - strand, within Pf1N1B4_5980 at 429.859 kb on - strand at 429.859 kb on - strand at 429.906 kb on + strand at 429.907 kb on - strand at 429.907 kb on - strand at 429.907 kb on - strand at 429.907 kb on - strand at 429.907 kb on - strand at 429.907 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 37C remove 427,141 - Pf1N1B4_5978 0.46 -0.4 427,442 + Pf1N1B4_5978 0.65 -0.2 427,483 - Pf1N1B4_5978 0.67 +0.9 428,145 + -0.7 428,146 - -0.4 428,146 - -1.5 428,361 + Pf1N1B4_5979 0.24 -1.0 428,362 - Pf1N1B4_5979 0.24 -1.9 428,362 - Pf1N1B4_5979 0.24 -2.6 428,711 - Pf1N1B4_5979 0.59 -0.7 428,711 - Pf1N1B4_5979 0.59 +0.3 429,214 + -1.3 429,236 - +0.6 429,684 - Pf1N1B4_5980 0.67 -0.6 429,859 - -0.6 429,859 - -0.2 429,906 + -1.9 429,907 - -1.5 429,907 - +1.4 429,907 - +1.1 429,907 - -2.7 429,907 - -1.7 429,907 - -0.1
Or see this region's nucleotide sequence