Experiment: LB 37C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_551 and Pf1N1B4_552 are separated by 123 nucleotides Pf1N1B4_552 and Pf1N1B4_553 are separated by 101 nucleotides Pf1N1B4_553 and Pf1N1B4_554 are separated by 66 nucleotides
Pf1N1B4_551 - Lipid A export ATP-binding/permease protein MsbA, at 647,752 to 649,584
_551
Pf1N1B4_552 - Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1), at 649,708 to 650,196
_552
Pf1N1B4_553 - Bacterial transcription activator, effector binding protein, at 650,298 to 650,786
_553
Pf1N1B4_554 - Putative threonine efflux protein, at 650,853 to 651,515
_554
Position (kb) 649
650
651 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 648.985 kb on + strand, within Pf1N1B4_551 at 648.985 kb on + strand, within Pf1N1B4_551 at 648.985 kb on + strand, within Pf1N1B4_551 at 649.534 kb on + strand at 649.535 kb on - strand at 649.772 kb on + strand, within Pf1N1B4_552 at 649.773 kb on - strand, within Pf1N1B4_552 at 649.773 kb on - strand, within Pf1N1B4_552 at 649.773 kb on - strand, within Pf1N1B4_552 at 649.793 kb on + strand, within Pf1N1B4_552 at 649.793 kb on + strand, within Pf1N1B4_552 at 649.793 kb on + strand, within Pf1N1B4_552 at 649.794 kb on - strand, within Pf1N1B4_552 at 649.803 kb on + strand, within Pf1N1B4_552 at 649.804 kb on - strand, within Pf1N1B4_552 at 649.905 kb on - strand, within Pf1N1B4_552 at 650.147 kb on + strand, within Pf1N1B4_552 at 650.147 kb on + strand, within Pf1N1B4_552 at 650.147 kb on + strand, within Pf1N1B4_552 at 650.147 kb on + strand, within Pf1N1B4_552 at 650.148 kb on - strand at 650.148 kb on - strand at 650.148 kb on - strand at 650.233 kb on + strand at 650.233 kb on + strand at 650.233 kb on + strand at 650.233 kb on + strand at 650.299 kb on + strand at 650.300 kb on - strand at 650.300 kb on - strand at 650.300 kb on - strand at 650.307 kb on + strand at 650.365 kb on + strand, within Pf1N1B4_553 at 650.366 kb on - strand, within Pf1N1B4_553 at 650.431 kb on + strand, within Pf1N1B4_553 at 650.431 kb on + strand, within Pf1N1B4_553 at 650.431 kb on + strand, within Pf1N1B4_553 at 650.432 kb on - strand, within Pf1N1B4_553 at 650.432 kb on - strand, within Pf1N1B4_553 at 650.432 kb on - strand, within Pf1N1B4_553 at 650.432 kb on - strand, within Pf1N1B4_553 at 650.432 kb on - strand, within Pf1N1B4_553 at 650.432 kb on - strand, within Pf1N1B4_553 at 650.432 kb on - strand, within Pf1N1B4_553 at 650.486 kb on - strand, within Pf1N1B4_553 at 650.486 kb on - strand, within Pf1N1B4_553 at 650.488 kb on + strand, within Pf1N1B4_553 at 650.489 kb on - strand, within Pf1N1B4_553 at 650.499 kb on + strand, within Pf1N1B4_553 at 650.500 kb on - strand, within Pf1N1B4_553 at 650.500 kb on - strand, within Pf1N1B4_553 at 650.500 kb on - strand, within Pf1N1B4_553 at 650.500 kb on - strand, within Pf1N1B4_553 at 650.515 kb on + strand, within Pf1N1B4_553 at 650.563 kb on + strand, within Pf1N1B4_553 at 650.564 kb on - strand, within Pf1N1B4_553 at 650.592 kb on - strand, within Pf1N1B4_553 at 650.605 kb on + strand, within Pf1N1B4_553 at 650.605 kb on + strand, within Pf1N1B4_553 at 650.606 kb on - strand, within Pf1N1B4_553 at 650.827 kb on - strand at 650.827 kb on - strand at 650.828 kb on + strand at 650.828 kb on + strand at 650.828 kb on + strand at 650.828 kb on + strand at 650.828 kb on + strand at 650.828 kb on + strand at 650.828 kb on + strand at 650.829 kb on - strand at 650.829 kb on - strand at 650.829 kb on - strand at 650.829 kb on - strand at 650.829 kb on - strand at 650.829 kb on - strand at 650.829 kb on - strand at 650.855 kb on - strand at 650.855 kb on - strand at 650.855 kb on - strand at 650.900 kb on + strand at 650.900 kb on + strand at 650.901 kb on - strand at 650.901 kb on - strand at 650.933 kb on - strand, within Pf1N1B4_554 at 650.966 kb on - strand, within Pf1N1B4_554
Per-strain Table
Position Strand Gene LocusTag Fraction LB 37C remove 648,985 + Pf1N1B4_551 0.67 -1.7 648,985 + Pf1N1B4_551 0.67 -0.9 648,985 + Pf1N1B4_551 0.67 +2.7 649,534 + -2.6 649,535 - -0.1 649,772 + Pf1N1B4_552 0.13 +0.3 649,773 - Pf1N1B4_552 0.13 -1.6 649,773 - Pf1N1B4_552 0.13 -2.0 649,773 - Pf1N1B4_552 0.13 -0.9 649,793 + Pf1N1B4_552 0.17 -0.3 649,793 + Pf1N1B4_552 0.17 +1.4 649,793 + Pf1N1B4_552 0.17 -1.4 649,794 - Pf1N1B4_552 0.18 +0.5 649,803 + Pf1N1B4_552 0.19 -1.7 649,804 - Pf1N1B4_552 0.20 -2.2 649,905 - Pf1N1B4_552 0.40 -1.2 650,147 + Pf1N1B4_552 0.90 -1.1 650,147 + Pf1N1B4_552 0.90 -2.9 650,147 + Pf1N1B4_552 0.90 -2.1 650,147 + Pf1N1B4_552 0.90 -2.2 650,148 - -0.9 650,148 - -1.6 650,148 - +1.9 650,233 + -1.9 650,233 + -2.8 650,233 + -1.3 650,233 + +0.8 650,299 + -1.1 650,300 - -0.1 650,300 - -0.7 650,300 - -1.1 650,307 + +0.7 650,365 + Pf1N1B4_553 0.14 -1.7 650,366 - Pf1N1B4_553 0.14 -0.1 650,431 + Pf1N1B4_553 0.27 -0.2 650,431 + Pf1N1B4_553 0.27 -0.6 650,431 + Pf1N1B4_553 0.27 +2.0 650,432 - Pf1N1B4_553 0.27 -1.4 650,432 - Pf1N1B4_553 0.27 -0.7 650,432 - Pf1N1B4_553 0.27 +1.6 650,432 - Pf1N1B4_553 0.27 -0.3 650,432 - Pf1N1B4_553 0.27 +0.0 650,432 - Pf1N1B4_553 0.27 -0.9 650,432 - Pf1N1B4_553 0.27 -1.7 650,486 - Pf1N1B4_553 0.38 -1.4 650,486 - Pf1N1B4_553 0.38 +2.0 650,488 + Pf1N1B4_553 0.39 +1.2 650,489 - Pf1N1B4_553 0.39 -2.4 650,499 + Pf1N1B4_553 0.41 +2.1 650,500 - Pf1N1B4_553 0.41 +1.6 650,500 - Pf1N1B4_553 0.41 -1.6 650,500 - Pf1N1B4_553 0.41 -1.7 650,500 - Pf1N1B4_553 0.41 +0.1 650,515 + Pf1N1B4_553 0.44 -1.2 650,563 + Pf1N1B4_553 0.54 +1.4 650,564 - Pf1N1B4_553 0.54 +2.0 650,592 - Pf1N1B4_553 0.60 -0.5 650,605 + Pf1N1B4_553 0.63 -0.6 650,605 + Pf1N1B4_553 0.63 -2.0 650,606 - Pf1N1B4_553 0.63 +0.9 650,827 - +0.5 650,827 - +2.2 650,828 + -1.1 650,828 + +0.4 650,828 + -1.3 650,828 + -1.1 650,828 + -1.0 650,828 + -0.4 650,828 + -1.9 650,829 - -1.3 650,829 - -2.0 650,829 - -1.3 650,829 - -0.8 650,829 - -0.1 650,829 - +0.4 650,829 - -1.5 650,855 - -0.3 650,855 - +0.9 650,855 - -0.6 650,900 + +0.7 650,900 + -1.9 650,901 - +0.4 650,901 - +0.8 650,933 - Pf1N1B4_554 0.12 -0.1 650,966 - Pf1N1B4_554 0.17 +0.7
Or see this region's nucleotide sequence