Experiment: LB 37C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5514 and Pf1N1B4_5515 are separated by 50 nucleotides Pf1N1B4_5515 and Pf1N1B4_5516 are separated by 802 nucleotides Pf1N1B4_5516 and Pf1N1B4_5517 overlap by 21 nucleotides
Pf1N1B4_5514 - RecA protein, at 261,587 to 262,636
_5514
Pf1N1B4_5515 - ATP-dependent DNA helicase pcrA (EC 3.6.1.-), at 262,687 to 266,067
_5515
Pf1N1B4_5516 - hypothetical protein, at 266,870 to 267,001
_5516
Pf1N1B4_5517 - RecB family exonuclease, at 266,981 to 269,668
_5517
Position (kb) 262
263
264
265
266
267 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 261.712 kb on - strand, within Pf1N1B4_5514 at 261.712 kb on - strand, within Pf1N1B4_5514 at 261.712 kb on - strand, within Pf1N1B4_5514 at 261.712 kb on - strand, within Pf1N1B4_5514 at 261.712 kb on - strand, within Pf1N1B4_5514 at 261.712 kb on - strand, within Pf1N1B4_5514 at 261.712 kb on - strand, within Pf1N1B4_5514 at 261.756 kb on + strand, within Pf1N1B4_5514 at 261.757 kb on - strand, within Pf1N1B4_5514 at 261.757 kb on - strand, within Pf1N1B4_5514 at 261.757 kb on - strand, within Pf1N1B4_5514 at 261.757 kb on - strand, within Pf1N1B4_5514 at 261.757 kb on - strand, within Pf1N1B4_5514 at 261.757 kb on - strand, within Pf1N1B4_5514 at 261.757 kb on - strand, within Pf1N1B4_5514 at 261.763 kb on - strand, within Pf1N1B4_5514 at 261.843 kb on + strand, within Pf1N1B4_5514 at 261.843 kb on + strand, within Pf1N1B4_5514 at 261.844 kb on - strand, within Pf1N1B4_5514 at 261.844 kb on - strand, within Pf1N1B4_5514 at 261.844 kb on - strand, within Pf1N1B4_5514 at 261.844 kb on - strand, within Pf1N1B4_5514 at 261.844 kb on - strand, within Pf1N1B4_5514 at 261.847 kb on + strand at 261.848 kb on - strand, within Pf1N1B4_5514 at 261.848 kb on - strand, within Pf1N1B4_5514 at 261.848 kb on - strand, within Pf1N1B4_5514 at 261.848 kb on - strand, within Pf1N1B4_5514 at 261.981 kb on + strand, within Pf1N1B4_5514 at 261.981 kb on + strand, within Pf1N1B4_5514 at 261.981 kb on + strand, within Pf1N1B4_5514 at 261.981 kb on + strand, within Pf1N1B4_5514 at 261.981 kb on + strand, within Pf1N1B4_5514 at 261.981 kb on + strand, within Pf1N1B4_5514 at 261.982 kb on - strand, within Pf1N1B4_5514 at 261.982 kb on - strand, within Pf1N1B4_5514 at 261.982 kb on - strand, within Pf1N1B4_5514 at 261.982 kb on - strand, within Pf1N1B4_5514 at 262.307 kb on + strand, within Pf1N1B4_5514 at 262.308 kb on - strand, within Pf1N1B4_5514 at 262.326 kb on + strand, within Pf1N1B4_5514 at 262.561 kb on + strand at 262.562 kb on - strand at 262.562 kb on - strand at 262.562 kb on - strand at 262.562 kb on - strand at 262.562 kb on - strand at 262.562 kb on - strand at 262.682 kb on - strand at 262.684 kb on - strand at 262.689 kb on - strand at 262.689 kb on - strand at 262.689 kb on - strand at 262.796 kb on - strand at 262.824 kb on - strand at 262.844 kb on + strand at 262.845 kb on - strand at 262.845 kb on - strand at 262.845 kb on - strand at 262.845 kb on - strand at 262.848 kb on + strand at 262.848 kb on + strand at 262.848 kb on + strand at 262.848 kb on + strand at 262.849 kb on - strand at 262.849 kb on - strand at 262.849 kb on - strand at 262.849 kb on - strand at 262.849 kb on - strand at 262.849 kb on - strand at 262.849 kb on - strand at 262.849 kb on - strand at 262.849 kb on - strand at 262.849 kb on - strand at 262.849 kb on - strand at 262.849 kb on - strand at 262.849 kb on - strand at 262.852 kb on + strand at 262.853 kb on - strand at 262.853 kb on - strand at 262.853 kb on - strand at 262.853 kb on - strand at 262.853 kb on - strand at 262.853 kb on - strand at 262.853 kb on - strand at 262.986 kb on - strand at 263.216 kb on - strand, within Pf1N1B4_5515 at 263.225 kb on + strand, within Pf1N1B4_5515 at 263.506 kb on - strand, within Pf1N1B4_5515 at 263.541 kb on - strand, within Pf1N1B4_5515 at 263.541 kb on - strand, within Pf1N1B4_5515 at 263.541 kb on - strand, within Pf1N1B4_5515 at 263.541 kb on - strand at 263.541 kb on - strand, within Pf1N1B4_5515 at 263.541 kb on - strand, within Pf1N1B4_5515 at 263.541 kb on - strand, within Pf1N1B4_5515 at 263.573 kb on + strand, within Pf1N1B4_5515 at 263.574 kb on - strand, within Pf1N1B4_5515 at 263.574 kb on - strand, within Pf1N1B4_5515 at 263.574 kb on - strand, within Pf1N1B4_5515 at 263.714 kb on + strand, within Pf1N1B4_5515 at 263.840 kb on + strand, within Pf1N1B4_5515 at 263.840 kb on + strand, within Pf1N1B4_5515 at 263.840 kb on + strand, within Pf1N1B4_5515 at 263.841 kb on - strand, within Pf1N1B4_5515 at 264.145 kb on - strand, within Pf1N1B4_5515 at 264.145 kb on - strand, within Pf1N1B4_5515 at 264.175 kb on + strand, within Pf1N1B4_5515 at 264.175 kb on + strand at 264.176 kb on - strand, within Pf1N1B4_5515 at 264.176 kb on - strand, within Pf1N1B4_5515 at 264.198 kb on + strand, within Pf1N1B4_5515 at 264.199 kb on - strand at 264.199 kb on - strand, within Pf1N1B4_5515 at 264.213 kb on + strand, within Pf1N1B4_5515 at 264.214 kb on - strand, within Pf1N1B4_5515 at 264.453 kb on + strand, within Pf1N1B4_5515 at 264.454 kb on - strand, within Pf1N1B4_5515 at 264.454 kb on - strand, within Pf1N1B4_5515 at 264.454 kb on - strand, within Pf1N1B4_5515 at 264.483 kb on - strand, within Pf1N1B4_5515 at 264.542 kb on + strand, within Pf1N1B4_5515 at 264.542 kb on + strand, within Pf1N1B4_5515 at 264.542 kb on + strand, within Pf1N1B4_5515 at 264.543 kb on - strand, within Pf1N1B4_5515 at 264.618 kb on + strand, within Pf1N1B4_5515 at 264.683 kb on + strand, within Pf1N1B4_5515 at 264.683 kb on + strand, within Pf1N1B4_5515 at 264.683 kb on + strand, within Pf1N1B4_5515 at 264.683 kb on + strand, within Pf1N1B4_5515 at 264.683 kb on + strand, within Pf1N1B4_5515 at 264.684 kb on - strand, within Pf1N1B4_5515 at 264.684 kb on - strand, within Pf1N1B4_5515 at 264.684 kb on - strand, within Pf1N1B4_5515 at 264.684 kb on - strand, within Pf1N1B4_5515 at 264.684 kb on - strand, within Pf1N1B4_5515 at 264.757 kb on + strand, within Pf1N1B4_5515 at 264.757 kb on + strand, within Pf1N1B4_5515 at 264.758 kb on - strand, within Pf1N1B4_5515 at 264.767 kb on + strand, within Pf1N1B4_5515 at 264.768 kb on - strand, within Pf1N1B4_5515 at 264.768 kb on - strand, within Pf1N1B4_5515 at 264.768 kb on - strand, within Pf1N1B4_5515 at 264.768 kb on - strand, within Pf1N1B4_5515 at 264.768 kb on - strand, within Pf1N1B4_5515 at 264.768 kb on - strand, within Pf1N1B4_5515 at 265.060 kb on - strand, within Pf1N1B4_5515 at 265.192 kb on - strand, within Pf1N1B4_5515 at 265.273 kb on + strand, within Pf1N1B4_5515 at 265.307 kb on + strand, within Pf1N1B4_5515 at 265.308 kb on - strand, within Pf1N1B4_5515 at 265.308 kb on - strand, within Pf1N1B4_5515 at 265.387 kb on + strand, within Pf1N1B4_5515 at 265.525 kb on + strand, within Pf1N1B4_5515 at 265.584 kb on + strand, within Pf1N1B4_5515 at 265.640 kb on + strand, within Pf1N1B4_5515 at 265.640 kb on + strand, within Pf1N1B4_5515 at 265.640 kb on + strand, within Pf1N1B4_5515 at 265.640 kb on + strand at 265.640 kb on + strand, within Pf1N1B4_5515 at 265.640 kb on + strand, within Pf1N1B4_5515 at 265.640 kb on + strand, within Pf1N1B4_5515 at 265.641 kb on - strand, within Pf1N1B4_5515 at 265.641 kb on - strand, within Pf1N1B4_5515 at 265.641 kb on - strand, within Pf1N1B4_5515 at 265.641 kb on - strand, within Pf1N1B4_5515 at 265.644 kb on + strand, within Pf1N1B4_5515 at 265.979 kb on - strand at 266.022 kb on + strand at 266.022 kb on + strand at 266.091 kb on - strand at 266.140 kb on - strand at 266.140 kb on - strand at 266.155 kb on + strand at 266.207 kb on - strand at 266.209 kb on - strand at 266.265 kb on - strand at 266.318 kb on + strand at 266.318 kb on + strand at 266.319 kb on - strand at 266.370 kb on + strand at 266.385 kb on + strand at 266.386 kb on - strand at 266.408 kb on - strand at 266.450 kb on - strand at 266.471 kb on + strand at 266.471 kb on + strand at 266.472 kb on - strand at 266.472 kb on - strand at 266.472 kb on - strand at 266.472 kb on - strand at 266.476 kb on + strand at 266.476 kb on + strand at 266.612 kb on + strand at 266.707 kb on + strand at 266.707 kb on + strand at 266.708 kb on - strand at 266.758 kb on + strand at 266.759 kb on - strand at 266.767 kb on + strand at 266.786 kb on + strand at 266.786 kb on + strand at 266.786 kb on + strand at 266.786 kb on + strand at 266.787 kb on - strand at 266.807 kb on + strand at 266.858 kb on + strand at 266.858 kb on + strand at 266.876 kb on + strand at 266.876 kb on + strand at 266.877 kb on - strand at 266.877 kb on - strand at 266.877 kb on - strand at 266.877 kb on - strand at 266.983 kb on - strand at 266.999 kb on + strand at 266.999 kb on + strand at 267.004 kb on - strand at 267.004 kb on - strand at 267.004 kb on - strand at 267.004 kb on - strand at 267.004 kb on - strand at 267.004 kb on - strand at 267.004 kb on - strand at 267.004 kb on - strand at 267.028 kb on + strand at 267.028 kb on + strand at 267.028 kb on + strand at 267.028 kb on + strand at 267.029 kb on - strand at 267.029 kb on - strand at 267.032 kb on + strand at 267.033 kb on - strand at 267.033 kb on - strand at 267.033 kb on - strand at 267.033 kb on - strand at 267.045 kb on + strand at 267.046 kb on - strand at 267.046 kb on - strand at 267.046 kb on - strand at 267.046 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 37C remove 261,712 - Pf1N1B4_5514 0.12 +0.4 261,712 - Pf1N1B4_5514 0.12 -0.9 261,712 - Pf1N1B4_5514 0.12 -0.5 261,712 - Pf1N1B4_5514 0.12 +1.8 261,712 - Pf1N1B4_5514 0.12 -0.7 261,712 - Pf1N1B4_5514 0.12 +1.8 261,712 - Pf1N1B4_5514 0.12 -1.1 261,756 + Pf1N1B4_5514 0.16 -2.8 261,757 - Pf1N1B4_5514 0.16 -1.1 261,757 - Pf1N1B4_5514 0.16 +0.4 261,757 - Pf1N1B4_5514 0.16 +0.7 261,757 - Pf1N1B4_5514 0.16 -0.8 261,757 - Pf1N1B4_5514 0.16 -0.8 261,757 - Pf1N1B4_5514 0.16 -0.1 261,757 - Pf1N1B4_5514 0.16 -0.4 261,763 - Pf1N1B4_5514 0.17 -2.2 261,843 + Pf1N1B4_5514 0.24 -1.2 261,843 + Pf1N1B4_5514 0.24 +1.0 261,844 - Pf1N1B4_5514 0.24 +2.3 261,844 - Pf1N1B4_5514 0.24 -0.8 261,844 - Pf1N1B4_5514 0.24 +1.7 261,844 - Pf1N1B4_5514 0.24 +2.0 261,844 - Pf1N1B4_5514 0.24 +1.0 261,847 + +0.2 261,848 - Pf1N1B4_5514 0.25 +0.1 261,848 - Pf1N1B4_5514 0.25 -1.4 261,848 - Pf1N1B4_5514 0.25 -0.1 261,848 - Pf1N1B4_5514 0.25 -1.2 261,981 + Pf1N1B4_5514 0.38 -0.5 261,981 + Pf1N1B4_5514 0.38 +0.2 261,981 + Pf1N1B4_5514 0.38 +2.6 261,981 + Pf1N1B4_5514 0.38 -0.4 261,981 + Pf1N1B4_5514 0.38 -0.0 261,981 + Pf1N1B4_5514 0.38 -0.6 261,982 - Pf1N1B4_5514 0.38 -1.1 261,982 - Pf1N1B4_5514 0.38 +0.8 261,982 - Pf1N1B4_5514 0.38 +0.9 261,982 - Pf1N1B4_5514 0.38 -1.1 262,307 + Pf1N1B4_5514 0.69 +1.0 262,308 - Pf1N1B4_5514 0.69 -0.4 262,326 + Pf1N1B4_5514 0.70 -0.4 262,561 + +0.7 262,562 - -1.4 262,562 - +0.8 262,562 - +1.6 262,562 - -0.1 262,562 - -2.3 262,562 - -1.8 262,682 - -1.2 262,684 - -0.7 262,689 - -0.8 262,689 - -1.0 262,689 - +1.8 262,796 - -2.5 262,824 - +0.0 262,844 + -0.7 262,845 - -1.1 262,845 - -1.3 262,845 - +0.6 262,845 - +0.8 262,848 + -1.3 262,848 + -2.2 262,848 + -0.5 262,848 + -1.5 262,849 - -1.8 262,849 - -1.4 262,849 - -1.7 262,849 - -0.5 262,849 - +0.8 262,849 - -0.5 262,849 - -0.6 262,849 - +0.7 262,849 - +0.2 262,849 - +1.1 262,849 - -1.5 262,849 - -0.2 262,849 - +0.2 262,852 + -1.0 262,853 - +1.7 262,853 - -1.0 262,853 - +3.1 262,853 - -1.4 262,853 - -1.9 262,853 - -0.7 262,853 - +0.2 262,986 - +0.1 263,216 - Pf1N1B4_5515 0.16 -0.9 263,225 + Pf1N1B4_5515 0.16 +3.2 263,506 - Pf1N1B4_5515 0.24 -1.4 263,541 - Pf1N1B4_5515 0.25 +2.4 263,541 - Pf1N1B4_5515 0.25 +1.6 263,541 - Pf1N1B4_5515 0.25 -0.8 263,541 - -0.5 263,541 - Pf1N1B4_5515 0.25 +1.1 263,541 - Pf1N1B4_5515 0.25 +0.6 263,541 - Pf1N1B4_5515 0.25 -0.1 263,573 + Pf1N1B4_5515 0.26 +0.5 263,574 - Pf1N1B4_5515 0.26 +1.3 263,574 - Pf1N1B4_5515 0.26 +0.4 263,574 - Pf1N1B4_5515 0.26 +0.8 263,714 + Pf1N1B4_5515 0.30 +2.1 263,840 + Pf1N1B4_5515 0.34 -0.9 263,840 + Pf1N1B4_5515 0.34 +1.0 263,840 + Pf1N1B4_5515 0.34 -1.0 263,841 - Pf1N1B4_5515 0.34 -1.1 264,145 - Pf1N1B4_5515 0.43 +0.6 264,145 - Pf1N1B4_5515 0.43 +0.6 264,175 + Pf1N1B4_5515 0.44 -1.4 264,175 + +1.8 264,176 - Pf1N1B4_5515 0.44 -2.1 264,176 - Pf1N1B4_5515 0.44 +2.0 264,198 + Pf1N1B4_5515 0.45 -0.5 264,199 - +1.2 264,199 - Pf1N1B4_5515 0.45 -1.4 264,213 + Pf1N1B4_5515 0.45 -0.5 264,214 - Pf1N1B4_5515 0.45 -0.8 264,453 + Pf1N1B4_5515 0.52 -2.2 264,454 - Pf1N1B4_5515 0.52 +0.6 264,454 - Pf1N1B4_5515 0.52 +1.6 264,454 - Pf1N1B4_5515 0.52 -2.1 264,483 - Pf1N1B4_5515 0.53 -0.7 264,542 + Pf1N1B4_5515 0.55 +0.3 264,542 + Pf1N1B4_5515 0.55 -0.5 264,542 + Pf1N1B4_5515 0.55 -1.1 264,543 - Pf1N1B4_5515 0.55 -2.0 264,618 + Pf1N1B4_5515 0.57 -0.1 264,683 + Pf1N1B4_5515 0.59 -0.8 264,683 + Pf1N1B4_5515 0.59 +1.9 264,683 + Pf1N1B4_5515 0.59 -1.3 264,683 + Pf1N1B4_5515 0.59 -0.4 264,683 + Pf1N1B4_5515 0.59 -3.1 264,684 - Pf1N1B4_5515 0.59 -0.0 264,684 - Pf1N1B4_5515 0.59 +0.3 264,684 - Pf1N1B4_5515 0.59 -0.5 264,684 - Pf1N1B4_5515 0.59 +0.3 264,684 - Pf1N1B4_5515 0.59 -1.2 264,757 + Pf1N1B4_5515 0.61 +0.3 264,757 + Pf1N1B4_5515 0.61 -1.8 264,758 - Pf1N1B4_5515 0.61 -0.8 264,767 + Pf1N1B4_5515 0.62 +0.1 264,768 - Pf1N1B4_5515 0.62 -1.3 264,768 - Pf1N1B4_5515 0.62 -1.3 264,768 - Pf1N1B4_5515 0.62 -0.8 264,768 - Pf1N1B4_5515 0.62 +0.2 264,768 - Pf1N1B4_5515 0.62 -1.5 264,768 - Pf1N1B4_5515 0.62 -0.7 265,060 - Pf1N1B4_5515 0.70 +2.4 265,192 - Pf1N1B4_5515 0.74 -0.4 265,273 + Pf1N1B4_5515 0.76 +1.4 265,307 + Pf1N1B4_5515 0.77 -0.9 265,308 - Pf1N1B4_5515 0.78 +0.3 265,308 - Pf1N1B4_5515 0.78 -1.8 265,387 + Pf1N1B4_5515 0.80 -0.9 265,525 + Pf1N1B4_5515 0.84 -0.5 265,584 + Pf1N1B4_5515 0.86 +0.6 265,640 + Pf1N1B4_5515 0.87 +0.4 265,640 + Pf1N1B4_5515 0.87 +0.5 265,640 + Pf1N1B4_5515 0.87 +0.1 265,640 + +0.8 265,640 + Pf1N1B4_5515 0.87 -0.9 265,640 + Pf1N1B4_5515 0.87 +0.9 265,640 + Pf1N1B4_5515 0.87 -0.7 265,641 - Pf1N1B4_5515 0.87 +1.3 265,641 - Pf1N1B4_5515 0.87 +0.9 265,641 - Pf1N1B4_5515 0.87 -1.6 265,641 - Pf1N1B4_5515 0.87 +1.1 265,644 + Pf1N1B4_5515 0.87 -2.9 265,979 - +1.2 266,022 + -0.8 266,022 + -1.5 266,091 - +2.2 266,140 - -1.1 266,140 - -1.4 266,155 + -0.5 266,207 - -2.4 266,209 - -0.2 266,265 - -3.1 266,318 + -1.0 266,318 + -1.8 266,319 - -1.9 266,370 + -0.0 266,385 + +1.5 266,386 - +1.1 266,408 - +1.5 266,450 - -0.7 266,471 + -0.7 266,471 + +0.1 266,472 - +3.1 266,472 - -1.7 266,472 - +0.3 266,472 - -0.9 266,476 + -0.2 266,476 + -0.4 266,612 + -0.7 266,707 + +0.2 266,707 + +0.5 266,708 - -0.4 266,758 + -2.1 266,759 - -0.4 266,767 + -0.1 266,786 + -0.5 266,786 + +0.3 266,786 + +1.9 266,786 + -0.4 266,787 - +1.7 266,807 + -1.5 266,858 + -1.2 266,858 + +1.6 266,876 + +0.8 266,876 + +0.8 266,877 - +0.9 266,877 - -0.9 266,877 - +1.3 266,877 - -0.4 266,983 - -0.4 266,999 + -0.8 266,999 + -0.0 267,004 - +0.3 267,004 - -1.1 267,004 - +1.2 267,004 - -1.0 267,004 - +0.9 267,004 - -0.0 267,004 - +0.1 267,004 - +0.2 267,028 + +0.7 267,028 + +0.6 267,028 + -0.0 267,028 + -0.8 267,029 - -0.9 267,029 - -1.7 267,032 + -1.2 267,033 - -1.4 267,033 - +1.7 267,033 - +1.2 267,033 - -1.4 267,045 + -1.1 267,046 - +0.8 267,046 - -1.3 267,046 - +1.5 267,046 - -1.1
Or see this region's nucleotide sequence