Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4999

Experiment: LB 37C

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4998 and Pf1N1B4_4999 are separated by 275 nucleotidesPf1N1B4_4999 and Pf1N1B4_5000 are separated by 169 nucleotides Pf1N1B4_4998 - Glycerate kinase (EC 2.7.1.31), at 5,546,605 to 5,547,747 _4998 Pf1N1B4_4999 - Sugar diacid utilization regulator SdaR, at 5,548,023 to 5,549,153 _4999 Pf1N1B4_5000 - D-glycerate transporter (predicted), at 5,549,323 to 5,550,633 _5000 Position (kb) 5548 5549 5550Strain fitness (log2 ratio) -1 0 1 2 3at 5547.798 kb on - strandat 5547.832 kb on + strandat 5547.833 kb on - strandat 5548.470 kb on - strand, within Pf1N1B4_4999at 5548.849 kb on + strand, within Pf1N1B4_4999at 5548.849 kb on + strandat 5548.849 kb on + strand, within Pf1N1B4_4999at 5548.849 kb on + strandat 5548.850 kb on - strand, within Pf1N1B4_4999at 5549.304 kb on - strandat 5549.304 kb on - strandat 5549.434 kb on + strandat 5549.543 kb on + strand, within Pf1N1B4_5000at 5549.543 kb on + strand, within Pf1N1B4_5000at 5549.543 kb on + strand, within Pf1N1B4_5000at 5549.543 kb on + strand, within Pf1N1B4_5000at 5549.544 kb on - strand, within Pf1N1B4_5000at 5549.544 kb on - strand, within Pf1N1B4_5000at 5549.935 kb on + strand, within Pf1N1B4_5000at 5549.956 kb on + strand, within Pf1N1B4_5000

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Per-strain Table

Position Strand Gene LocusTag Fraction LB 37C
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5,547,798 - -1.3
5,547,832 + +0.8
5,547,833 - -1.6
5,548,470 - Pf1N1B4_4999 0.40 +2.8
5,548,849 + Pf1N1B4_4999 0.73 -0.5
5,548,849 + -0.3
5,548,849 + Pf1N1B4_4999 0.73 +2.0
5,548,849 + +1.3
5,548,850 - Pf1N1B4_4999 0.73 -1.5
5,549,304 - -0.5
5,549,304 - +0.9
5,549,434 + +0.2
5,549,543 + Pf1N1B4_5000 0.17 -0.9
5,549,543 + Pf1N1B4_5000 0.17 -0.6
5,549,543 + Pf1N1B4_5000 0.17 -0.7
5,549,543 + Pf1N1B4_5000 0.17 -0.5
5,549,544 - Pf1N1B4_5000 0.17 -0.2
5,549,544 - Pf1N1B4_5000 0.17 -0.3
5,549,935 + Pf1N1B4_5000 0.47 -0.7
5,549,956 + Pf1N1B4_5000 0.48 +1.9

Or see this region's nucleotide sequence