Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4867

Experiment: LB 37C

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4865 and Pf1N1B4_4866 are separated by 307 nucleotidesPf1N1B4_4866 and Pf1N1B4_4867 are separated by 40 nucleotidesPf1N1B4_4867 and Pf1N1B4_4868 are separated by 2 nucleotides Pf1N1B4_4865 - Multidrug efflux membrane fusion protein MexE, at 5,382,967 to 5,384,220 _4865 Pf1N1B4_4866 - hypothetical protein, at 5,384,528 to 5,384,653 _4866 Pf1N1B4_4867 - FIG00464907: hypothetical protein, at 5,384,694 to 5,385,713 _4867 Pf1N1B4_4868 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 5,385,716 to 5,388,721 _4868 Position (kb) 5384 5385 5386Strain fitness (log2 ratio) -2 -1 0 1 2 3at 5384.239 kb on - strandat 5384.272 kb on + strandat 5384.272 kb on + strandat 5384.273 kb on - strandat 5384.273 kb on - strandat 5384.318 kb on + strandat 5384.320 kb on + strandat 5384.397 kb on + strandat 5384.529 kb on + strandat 5384.530 kb on - strandat 5384.530 kb on - strandat 5384.561 kb on + strand, within Pf1N1B4_4866at 5384.561 kb on + strand, within Pf1N1B4_4866at 5384.599 kb on + strand, within Pf1N1B4_4866at 5384.615 kb on - strand, within Pf1N1B4_4866at 5384.633 kb on + strand, within Pf1N1B4_4866at 5384.633 kb on + strand, within Pf1N1B4_4866at 5385.195 kb on + strand, within Pf1N1B4_4867at 5385.257 kb on + strand, within Pf1N1B4_4867at 5385.257 kb on + strand, within Pf1N1B4_4867at 5385.257 kb on + strandat 5385.257 kb on + strand, within Pf1N1B4_4867at 5385.258 kb on - strand, within Pf1N1B4_4867at 5385.258 kb on - strand, within Pf1N1B4_4867at 5385.381 kb on + strand, within Pf1N1B4_4867at 5385.381 kb on + strand, within Pf1N1B4_4867at 5385.382 kb on - strand, within Pf1N1B4_4867at 5385.485 kb on - strand, within Pf1N1B4_4867at 5385.498 kb on + strand, within Pf1N1B4_4867at 5385.690 kb on + strandat 5385.690 kb on + strandat 5385.691 kb on - strandat 5385.691 kb on - strandat 5385.691 kb on - strandat 5385.691 kb on - strandat 5385.724 kb on + strandat 5386.109 kb on - strand, within Pf1N1B4_4868at 5386.240 kb on - strand, within Pf1N1B4_4868at 5386.240 kb on - strand, within Pf1N1B4_4868at 5386.475 kb on + strand, within Pf1N1B4_4868at 5386.475 kb on + strandat 5386.476 kb on - strandat 5386.476 kb on - strand, within Pf1N1B4_4868at 5386.488 kb on - strand, within Pf1N1B4_4868at 5386.632 kb on + strand, within Pf1N1B4_4868at 5386.633 kb on - strand, within Pf1N1B4_4868

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Per-strain Table

Position Strand Gene LocusTag Fraction LB 37C
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5,384,239 - +1.0
5,384,272 + +0.3
5,384,272 + -1.1
5,384,273 - +0.0
5,384,273 - -0.5
5,384,318 + +0.3
5,384,320 + +0.2
5,384,397 + -0.1
5,384,529 + +1.3
5,384,530 - -1.9
5,384,530 - +0.2
5,384,561 + Pf1N1B4_4866 0.26 -1.9
5,384,561 + Pf1N1B4_4866 0.26 -1.7
5,384,599 + Pf1N1B4_4866 0.56 -0.1
5,384,615 - Pf1N1B4_4866 0.69 -1.9
5,384,633 + Pf1N1B4_4866 0.83 -0.5
5,384,633 + Pf1N1B4_4866 0.83 -0.3
5,385,195 + Pf1N1B4_4867 0.49 -0.1
5,385,257 + Pf1N1B4_4867 0.55 -0.6
5,385,257 + Pf1N1B4_4867 0.55 -1.4
5,385,257 + +1.4
5,385,257 + Pf1N1B4_4867 0.55 -0.3
5,385,258 - Pf1N1B4_4867 0.55 -1.7
5,385,258 - Pf1N1B4_4867 0.55 -0.5
5,385,381 + Pf1N1B4_4867 0.67 -0.7
5,385,381 + Pf1N1B4_4867 0.67 -1.0
5,385,382 - Pf1N1B4_4867 0.67 -2.2
5,385,485 - Pf1N1B4_4867 0.78 -1.7
5,385,498 + Pf1N1B4_4867 0.79 +0.7
5,385,690 + -1.5
5,385,690 + +0.2
5,385,691 - -1.8
5,385,691 - +0.9
5,385,691 - -0.6
5,385,691 - +2.1
5,385,724 + +0.1
5,386,109 - Pf1N1B4_4868 0.13 +2.9
5,386,240 - Pf1N1B4_4868 0.17 -0.7
5,386,240 - Pf1N1B4_4868 0.17 +0.7
5,386,475 + Pf1N1B4_4868 0.25 -1.2
5,386,475 + -0.3
5,386,476 - +0.3
5,386,476 - Pf1N1B4_4868 0.25 -2.2
5,386,488 - Pf1N1B4_4868 0.26 +1.4
5,386,632 + Pf1N1B4_4868 0.30 -1.5
5,386,633 - Pf1N1B4_4868 0.31 -1.4

Or see this region's nucleotide sequence