Experiment: LB 37C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3937 and Pf1N1B4_3938 are separated by 28 nucleotides Pf1N1B4_3938 and Pf1N1B4_3939 are separated by 79 nucleotides Pf1N1B4_3939 and Pf1N1B4_3940 are separated by 171 nucleotides
Pf1N1B4_3937 - Membrane protein involved in colicin uptake, at 4,374,450 to 4,374,692
_3937
Pf1N1B4_3938 - Integral membrane protein TerC, at 4,374,721 to 4,375,470
_3938
Pf1N1B4_3939 - FIG00953489: hypothetical protein, at 4,375,550 to 4,375,720
_3939
Pf1N1B4_3940 - Glutamate synthase [NADPH] large chain (EC 1.4.1.13), at 4,375,892 to 4,376,620
_3940
Position (kb)
4375
4376 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4374.690 kb on + strand at 4374.690 kb on + strand at 4374.699 kb on + strand at 4374.727 kb on + strand at 4374.734 kb on + strand at 4374.734 kb on + strand at 4374.734 kb on + strand at 4375.375 kb on + strand, within Pf1N1B4_3938 at 4375.375 kb on + strand, within Pf1N1B4_3938 at 4375.375 kb on + strand, within Pf1N1B4_3938 at 4375.376 kb on - strand, within Pf1N1B4_3938 at 4375.376 kb on - strand, within Pf1N1B4_3938 at 4375.376 kb on - strand, within Pf1N1B4_3938 at 4375.376 kb on - strand at 4375.478 kb on + strand at 4375.637 kb on - strand, within Pf1N1B4_3939 at 4375.640 kb on - strand at 4375.697 kb on + strand, within Pf1N1B4_3939 at 4375.697 kb on + strand, within Pf1N1B4_3939 at 4375.697 kb on + strand, within Pf1N1B4_3939 at 4375.698 kb on - strand, within Pf1N1B4_3939 at 4375.698 kb on - strand, within Pf1N1B4_3939 at 4375.698 kb on - strand at 4375.698 kb on - strand, within Pf1N1B4_3939 at 4375.698 kb on - strand at 4375.698 kb on - strand, within Pf1N1B4_3939 at 4375.698 kb on - strand at 4375.766 kb on + strand at 4375.790 kb on + strand at 4375.790 kb on + strand at 4375.790 kb on + strand at 4375.868 kb on + strand at 4375.891 kb on + strand at 4376.099 kb on + strand, within Pf1N1B4_3940 at 4376.672 kb on + strand at 4376.673 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 37C remove 4,374,690 + -1.2 4,374,690 + +2.4 4,374,699 + +0.4 4,374,727 + -1.7 4,374,734 + -1.0 4,374,734 + +0.3 4,374,734 + -1.2 4,375,375 + Pf1N1B4_3938 0.87 -2.7 4,375,375 + Pf1N1B4_3938 0.87 -2.1 4,375,375 + Pf1N1B4_3938 0.87 -1.9 4,375,376 - Pf1N1B4_3938 0.87 +2.6 4,375,376 - Pf1N1B4_3938 0.87 -1.2 4,375,376 - Pf1N1B4_3938 0.87 -0.8 4,375,376 - +2.0 4,375,478 + +0.6 4,375,637 - Pf1N1B4_3939 0.51 +0.6 4,375,640 - +1.4 4,375,697 + Pf1N1B4_3939 0.86 +0.2 4,375,697 + Pf1N1B4_3939 0.86 -0.5 4,375,697 + Pf1N1B4_3939 0.86 -1.8 4,375,698 - Pf1N1B4_3939 0.87 -0.6 4,375,698 - Pf1N1B4_3939 0.87 +1.4 4,375,698 - +3.1 4,375,698 - Pf1N1B4_3939 0.87 -1.4 4,375,698 - -0.4 4,375,698 - Pf1N1B4_3939 0.87 -1.4 4,375,698 - +0.3 4,375,766 + -0.1 4,375,790 + +0.5 4,375,790 + +0.1 4,375,790 + +0.7 4,375,868 + -0.7 4,375,891 + -0.6 4,376,099 + Pf1N1B4_3940 0.28 +1.2 4,376,672 + +1.5 4,376,673 - +0.2
Or see this region's nucleotide sequence