Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3542

Experiment: LB 37C

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3540 and Pf1N1B4_3541 are separated by 99 nucleotidesPf1N1B4_3541 and Pf1N1B4_3542 are separated by 191 nucleotidesPf1N1B4_3542 and Pf1N1B4_3543 overlap by 44 nucleotides Pf1N1B4_3540 - Metal-dependent phosphohydrolase, HD subdomain, at 3,946,960 to 3,947,550 _3540 Pf1N1B4_3541 - FIG027190: Putative transmembrane protein, at 3,947,650 to 3,947,961 _3541 Pf1N1B4_3542 - Transcriptional regulator, AraC family, at 3,948,153 to 3,949,181 _3542 Pf1N1B4_3543 - Potassium uptake protein TrkH, at 3,949,138 to 3,950,592 _3543 Position (kb) 3948 3949 3950Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 3947.186 kb on + strand, within Pf1N1B4_3540at 3947.186 kb on + strand, within Pf1N1B4_3540at 3947.186 kb on + strand, within Pf1N1B4_3540at 3947.187 kb on - strand, within Pf1N1B4_3540at 3947.187 kb on - strand, within Pf1N1B4_3540at 3947.187 kb on - strand, within Pf1N1B4_3540at 3947.187 kb on - strand, within Pf1N1B4_3540at 3947.190 kb on - strand, within Pf1N1B4_3540at 3947.282 kb on + strand, within Pf1N1B4_3540at 3947.330 kb on + strand, within Pf1N1B4_3540at 3947.330 kb on + strandat 3947.331 kb on - strand, within Pf1N1B4_3540at 3947.331 kb on - strand, within Pf1N1B4_3540at 3947.331 kb on - strand, within Pf1N1B4_3540at 3947.331 kb on - strand, within Pf1N1B4_3540at 3947.331 kb on - strand, within Pf1N1B4_3540at 3947.331 kb on - strand, within Pf1N1B4_3540at 3947.331 kb on - strandat 3947.425 kb on + strand, within Pf1N1B4_3540at 3947.523 kb on + strandat 3947.524 kb on - strandat 3947.592 kb on + strandat 3947.652 kb on - strandat 3947.652 kb on - strandat 3947.684 kb on + strand, within Pf1N1B4_3541at 3947.685 kb on - strand, within Pf1N1B4_3541at 3947.685 kb on - strand, within Pf1N1B4_3541at 3947.778 kb on + strandat 3947.778 kb on + strand, within Pf1N1B4_3541at 3947.778 kb on + strand, within Pf1N1B4_3541at 3947.778 kb on + strand, within Pf1N1B4_3541at 3947.779 kb on - strand, within Pf1N1B4_3541at 3947.779 kb on - strand, within Pf1N1B4_3541at 3947.779 kb on - strand, within Pf1N1B4_3541at 3947.779 kb on - strand, within Pf1N1B4_3541at 3947.913 kb on - strand, within Pf1N1B4_3541at 3948.015 kb on + strandat 3948.015 kb on + strandat 3948.016 kb on - strandat 3948.019 kb on + strandat 3948.020 kb on - strandat 3948.020 kb on - strandat 3948.057 kb on - strandat 3948.068 kb on + strandat 3948.069 kb on - strandat 3948.109 kb on + strandat 3948.113 kb on - strandat 3948.225 kb on + strandat 3948.483 kb on + strand, within Pf1N1B4_3542at 3948.648 kb on + strand, within Pf1N1B4_3542at 3948.648 kb on + strand, within Pf1N1B4_3542at 3948.648 kb on + strand, within Pf1N1B4_3542at 3948.648 kb on + strandat 3948.648 kb on + strand, within Pf1N1B4_3542at 3948.648 kb on + strand, within Pf1N1B4_3542at 3948.648 kb on + strand, within Pf1N1B4_3542at 3948.648 kb on + strand, within Pf1N1B4_3542at 3948.649 kb on - strand, within Pf1N1B4_3542at 3948.690 kb on + strand, within Pf1N1B4_3542at 3948.691 kb on - strandat 3948.691 kb on - strand, within Pf1N1B4_3542at 3948.786 kb on + strand, within Pf1N1B4_3542at 3948.787 kb on - strand, within Pf1N1B4_3542at 3948.838 kb on + strandat 3948.838 kb on + strand, within Pf1N1B4_3542at 3948.875 kb on + strand, within Pf1N1B4_3542at 3948.990 kb on + strand, within Pf1N1B4_3542at 3948.990 kb on + strandat 3948.990 kb on + strandat 3948.991 kb on - strand, within Pf1N1B4_3542at 3948.991 kb on - strand, within Pf1N1B4_3542at 3949.029 kb on + strandat 3949.139 kb on + strandat 3949.140 kb on - strandat 3949.179 kb on + strandat 3949.448 kb on + strand, within Pf1N1B4_3543at 3949.449 kb on - strand, within Pf1N1B4_3543at 3949.449 kb on - strand, within Pf1N1B4_3543at 3949.449 kb on - strand, within Pf1N1B4_3543at 3949.449 kb on - strand, within Pf1N1B4_3543at 3949.449 kb on - strand, within Pf1N1B4_3543at 3949.477 kb on + strand, within Pf1N1B4_3543at 3949.478 kb on - strand, within Pf1N1B4_3543at 3949.489 kb on - strand, within Pf1N1B4_3543at 3949.511 kb on + strand, within Pf1N1B4_3543at 3949.511 kb on + strand, within Pf1N1B4_3543at 3949.511 kb on + strand, within Pf1N1B4_3543at 3949.511 kb on + strand, within Pf1N1B4_3543at 3949.512 kb on - strand, within Pf1N1B4_3543at 3949.512 kb on - strand, within Pf1N1B4_3543at 3949.512 kb on - strand, within Pf1N1B4_3543at 3949.583 kb on + strand, within Pf1N1B4_3543at 3949.584 kb on - strand, within Pf1N1B4_3543at 3949.622 kb on + strand, within Pf1N1B4_3543at 3949.622 kb on + strand, within Pf1N1B4_3543at 3949.758 kb on + strand, within Pf1N1B4_3543at 3949.826 kb on + strand, within Pf1N1B4_3543at 3949.826 kb on + strand, within Pf1N1B4_3543at 3949.826 kb on + strand, within Pf1N1B4_3543at 3949.826 kb on + strand, within Pf1N1B4_3543at 3949.826 kb on + strand, within Pf1N1B4_3543at 3949.826 kb on + strand, within Pf1N1B4_3543at 3949.826 kb on + strand, within Pf1N1B4_3543at 3949.827 kb on - strand, within Pf1N1B4_3543at 3949.827 kb on - strand, within Pf1N1B4_3543at 3949.827 kb on - strand, within Pf1N1B4_3543at 3949.827 kb on - strand, within Pf1N1B4_3543at 3950.027 kb on + strand, within Pf1N1B4_3543at 3950.027 kb on + strand, within Pf1N1B4_3543at 3950.027 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB 37C
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3,947,186 + Pf1N1B4_3540 0.38 +1.0
3,947,186 + Pf1N1B4_3540 0.38 +0.2
3,947,186 + Pf1N1B4_3540 0.38 +1.6
3,947,187 - Pf1N1B4_3540 0.38 +0.9
3,947,187 - Pf1N1B4_3540 0.38 +0.1
3,947,187 - Pf1N1B4_3540 0.38 +0.7
3,947,187 - Pf1N1B4_3540 0.38 +1.6
3,947,190 - Pf1N1B4_3540 0.39 -0.0
3,947,282 + Pf1N1B4_3540 0.54 +2.0
3,947,330 + Pf1N1B4_3540 0.63 -1.3
3,947,330 + -0.0
3,947,331 - Pf1N1B4_3540 0.63 +0.0
3,947,331 - Pf1N1B4_3540 0.63 +0.2
3,947,331 - Pf1N1B4_3540 0.63 +0.1
3,947,331 - Pf1N1B4_3540 0.63 +1.9
3,947,331 - Pf1N1B4_3540 0.63 -0.9
3,947,331 - Pf1N1B4_3540 0.63 +0.5
3,947,331 - -0.0
3,947,425 + Pf1N1B4_3540 0.79 -2.6
3,947,523 + +1.1
3,947,524 - +0.3
3,947,592 + -0.7
3,947,652 - -0.7
3,947,652 - -0.7
3,947,684 + Pf1N1B4_3541 0.11 -0.1
3,947,685 - Pf1N1B4_3541 0.11 +1.1
3,947,685 - Pf1N1B4_3541 0.11 -1.2
3,947,778 + +1.0
3,947,778 + Pf1N1B4_3541 0.41 +0.3
3,947,778 + Pf1N1B4_3541 0.41 -1.5
3,947,778 + Pf1N1B4_3541 0.41 -0.4
3,947,779 - Pf1N1B4_3541 0.41 -1.0
3,947,779 - Pf1N1B4_3541 0.41 +0.1
3,947,779 - Pf1N1B4_3541 0.41 -0.6
3,947,779 - Pf1N1B4_3541 0.41 +0.3
3,947,913 - Pf1N1B4_3541 0.84 -0.0
3,948,015 + -0.6
3,948,015 + -2.2
3,948,016 - -0.2
3,948,019 + +0.3
3,948,020 - -0.7
3,948,020 - +2.3
3,948,057 - -1.0
3,948,068 + -0.7
3,948,069 - -1.4
3,948,109 + -1.5
3,948,113 - -0.1
3,948,225 + +0.8
3,948,483 + Pf1N1B4_3542 0.32 -1.3
3,948,648 + Pf1N1B4_3542 0.48 -1.9
3,948,648 + Pf1N1B4_3542 0.48 +1.1
3,948,648 + Pf1N1B4_3542 0.48 +1.0
3,948,648 + +2.8
3,948,648 + Pf1N1B4_3542 0.48 -0.0
3,948,648 + Pf1N1B4_3542 0.48 +1.4
3,948,648 + Pf1N1B4_3542 0.48 -0.8
3,948,648 + Pf1N1B4_3542 0.48 -2.2
3,948,649 - Pf1N1B4_3542 0.48 -0.0
3,948,690 + Pf1N1B4_3542 0.52 -1.4
3,948,691 - -0.0
3,948,691 - Pf1N1B4_3542 0.52 -0.7
3,948,786 + Pf1N1B4_3542 0.62 -1.3
3,948,787 - Pf1N1B4_3542 0.62 -0.4
3,948,838 + -0.2
3,948,838 + Pf1N1B4_3542 0.67 -0.8
3,948,875 + Pf1N1B4_3542 0.70 -0.2
3,948,990 + Pf1N1B4_3542 0.81 +0.8
3,948,990 + +0.3
3,948,990 + -0.0
3,948,991 - Pf1N1B4_3542 0.81 -2.3
3,948,991 - Pf1N1B4_3542 0.81 -2.9
3,949,029 + -0.0
3,949,139 + -1.2
3,949,140 - -1.3
3,949,179 + -0.2
3,949,448 + Pf1N1B4_3543 0.21 +0.1
3,949,449 - Pf1N1B4_3543 0.21 +3.5
3,949,449 - Pf1N1B4_3543 0.21 -2.7
3,949,449 - Pf1N1B4_3543 0.21 -0.9
3,949,449 - Pf1N1B4_3543 0.21 +0.4
3,949,449 - Pf1N1B4_3543 0.21 +0.3
3,949,477 + Pf1N1B4_3543 0.23 -0.7
3,949,478 - Pf1N1B4_3543 0.23 -1.9
3,949,489 - Pf1N1B4_3543 0.24 +0.4
3,949,511 + Pf1N1B4_3543 0.26 +0.1
3,949,511 + Pf1N1B4_3543 0.26 -1.4
3,949,511 + Pf1N1B4_3543 0.26 -1.1
3,949,511 + Pf1N1B4_3543 0.26 +3.2
3,949,512 - Pf1N1B4_3543 0.26 -1.1
3,949,512 - Pf1N1B4_3543 0.26 -1.0
3,949,512 - Pf1N1B4_3543 0.26 +0.7
3,949,583 + Pf1N1B4_3543 0.31 +1.4
3,949,584 - Pf1N1B4_3543 0.31 -1.4
3,949,622 + Pf1N1B4_3543 0.33 -0.7
3,949,622 + Pf1N1B4_3543 0.33 -0.2
3,949,758 + Pf1N1B4_3543 0.43 -1.8
3,949,826 + Pf1N1B4_3543 0.47 +0.4
3,949,826 + Pf1N1B4_3543 0.47 +0.1
3,949,826 + Pf1N1B4_3543 0.47 +1.1
3,949,826 + Pf1N1B4_3543 0.47 -1.7
3,949,826 + Pf1N1B4_3543 0.47 +0.4
3,949,826 + Pf1N1B4_3543 0.47 -0.7
3,949,826 + Pf1N1B4_3543 0.47 -2.3
3,949,827 - Pf1N1B4_3543 0.47 -1.0
3,949,827 - Pf1N1B4_3543 0.47 -1.0
3,949,827 - Pf1N1B4_3543 0.47 -0.5
3,949,827 - Pf1N1B4_3543 0.47 -1.7
3,950,027 + Pf1N1B4_3543 0.61 -0.9
3,950,027 + Pf1N1B4_3543 0.61 -0.6
3,950,027 + -0.0

Or see this region's nucleotide sequence