Experiment: LB 37C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3523 and Pf1N1B4_3524 are separated by 110 nucleotides Pf1N1B4_3524 and Pf1N1B4_3525 overlap by 4 nucleotides Pf1N1B4_3525 and Pf1N1B4_3526 are separated by 50 nucleotides Pf1N1B4_3526 and Pf1N1B4_3527 are separated by 8 nucleotides
Pf1N1B4_3523 - hypothetical protein, at 3,929,988 to 3,930,125
_3523
Pf1N1B4_3524 - FIG00955363: hypothetical protein, at 3,930,236 to 3,931,042
_3524
Pf1N1B4_3525 - hydrolase, alpha/beta fold family, at 3,931,039 to 3,931,893
_3525
Pf1N1B4_3526 - Glutathione S-transferase (EC 2.5.1.18), at 3,931,944 to 3,932,603
_3526
Pf1N1B4_3527 - Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), A subunit, at 3,932,612 to 3,933,418
_3527
Position (kb)
3931
3932 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3930.133 kb on + strand at 3930.181 kb on + strand at 3930.235 kb on + strand at 3930.235 kb on + strand at 3930.235 kb on + strand at 3930.235 kb on + strand at 3930.236 kb on - strand at 3930.236 kb on - strand at 3930.236 kb on - strand at 3930.236 kb on - strand at 3930.249 kb on + strand at 3930.250 kb on - strand at 3930.250 kb on - strand at 3930.250 kb on - strand at 3930.288 kb on - strand at 3930.288 kb on - strand at 3930.321 kb on - strand, within Pf1N1B4_3524 at 3930.674 kb on - strand at 3930.674 kb on - strand, within Pf1N1B4_3524 at 3930.716 kb on + strand, within Pf1N1B4_3524 at 3930.716 kb on + strand, within Pf1N1B4_3524 at 3930.791 kb on + strand, within Pf1N1B4_3524 at 3930.792 kb on - strand, within Pf1N1B4_3524 at 3930.792 kb on - strand, within Pf1N1B4_3524 at 3930.792 kb on - strand, within Pf1N1B4_3524 at 3930.949 kb on + strand, within Pf1N1B4_3524 at 3930.949 kb on + strand, within Pf1N1B4_3524 at 3930.949 kb on + strand, within Pf1N1B4_3524 at 3931.299 kb on - strand, within Pf1N1B4_3525 at 3931.431 kb on + strand, within Pf1N1B4_3525 at 3931.516 kb on + strand at 3931.516 kb on + strand, within Pf1N1B4_3525 at 3931.516 kb on + strand, within Pf1N1B4_3525 at 3931.629 kb on + strand, within Pf1N1B4_3525 at 3931.629 kb on + strand, within Pf1N1B4_3525 at 3931.630 kb on - strand at 3931.948 kb on + strand at 3931.948 kb on + strand at 3931.948 kb on + strand at 3931.949 kb on - strand at 3931.949 kb on - strand at 3931.956 kb on + strand at 3931.956 kb on + strand at 3932.164 kb on - strand, within Pf1N1B4_3526 at 3932.352 kb on + strand, within Pf1N1B4_3526 at 3932.352 kb on + strand, within Pf1N1B4_3526 at 3932.352 kb on + strand, within Pf1N1B4_3526 at 3932.352 kb on + strand, within Pf1N1B4_3526 at 3932.352 kb on + strand, within Pf1N1B4_3526 at 3932.352 kb on + strand, within Pf1N1B4_3526 at 3932.352 kb on + strand, within Pf1N1B4_3526 at 3932.403 kb on + strand, within Pf1N1B4_3526 at 3932.427 kb on + strand, within Pf1N1B4_3526 at 3932.608 kb on - strand at 3932.672 kb on - strand at 3932.849 kb on + strand at 3932.850 kb on - strand, within Pf1N1B4_3527 at 3932.850 kb on - strand, within Pf1N1B4_3527 at 3932.850 kb on - strand, within Pf1N1B4_3527
Per-strain Table
Position Strand Gene LocusTag Fraction LB 37C remove 3,930,133 + -2.5 3,930,181 + +0.6 3,930,235 + -0.9 3,930,235 + -1.3 3,930,235 + +0.6 3,930,235 + +0.4 3,930,236 - -0.2 3,930,236 - -2.1 3,930,236 - -1.3 3,930,236 - -2.2 3,930,249 + -1.2 3,930,250 - -0.2 3,930,250 - -2.0 3,930,250 - -1.4 3,930,288 - +0.4 3,930,288 - -2.1 3,930,321 - Pf1N1B4_3524 0.11 -1.3 3,930,674 - +0.6 3,930,674 - Pf1N1B4_3524 0.54 +1.3 3,930,716 + Pf1N1B4_3524 0.59 -2.6 3,930,716 + Pf1N1B4_3524 0.59 -1.3 3,930,791 + Pf1N1B4_3524 0.69 -1.2 3,930,792 - Pf1N1B4_3524 0.69 -0.7 3,930,792 - Pf1N1B4_3524 0.69 +0.6 3,930,792 - Pf1N1B4_3524 0.69 -0.8 3,930,949 + Pf1N1B4_3524 0.88 +2.2 3,930,949 + Pf1N1B4_3524 0.88 -1.3 3,930,949 + Pf1N1B4_3524 0.88 -0.0 3,931,299 - Pf1N1B4_3525 0.30 +3.2 3,931,431 + Pf1N1B4_3525 0.46 +1.6 3,931,516 + -0.8 3,931,516 + Pf1N1B4_3525 0.56 +1.5 3,931,516 + Pf1N1B4_3525 0.56 -1.2 3,931,629 + Pf1N1B4_3525 0.69 -2.9 3,931,629 + Pf1N1B4_3525 0.69 +1.6 3,931,630 - -0.2 3,931,948 + +0.3 3,931,948 + -0.3 3,931,948 + +0.8 3,931,949 - -2.5 3,931,949 - -1.1 3,931,956 + +1.8 3,931,956 + -2.2 3,932,164 - Pf1N1B4_3526 0.33 -2.3 3,932,352 + Pf1N1B4_3526 0.62 +1.6 3,932,352 + Pf1N1B4_3526 0.62 -2.1 3,932,352 + Pf1N1B4_3526 0.62 +0.4 3,932,352 + Pf1N1B4_3526 0.62 -0.4 3,932,352 + Pf1N1B4_3526 0.62 +1.3 3,932,352 + Pf1N1B4_3526 0.62 +1.1 3,932,352 + Pf1N1B4_3526 0.62 -1.8 3,932,403 + Pf1N1B4_3526 0.70 -0.3 3,932,427 + Pf1N1B4_3526 0.73 -2.1 3,932,608 - +0.4 3,932,672 - -0.2 3,932,849 + -0.2 3,932,850 - Pf1N1B4_3527 0.29 -0.8 3,932,850 - Pf1N1B4_3527 0.29 -0.3 3,932,850 - Pf1N1B4_3527 0.29 -0.2
Or see this region's nucleotide sequence