Experiment: LB 37C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_317 and Pf1N1B4_318 are separated by 97 nucleotides Pf1N1B4_318 and Pf1N1B4_319 are separated by 15 nucleotides Pf1N1B4_319 and Pf1N1B4_320 are separated by 47 nucleotides
Pf1N1B4_317 - FIG00956575: hypothetical protein, at 374,497 to 374,664
_317
Pf1N1B4_318 - NOHBY730; no homolog in Saccharomyces cerevisiae, at 374,762 to 375,613
_318
Pf1N1B4_319 - Transcriptional regulator, GntR family, at 375,629 to 376,306
_319
Pf1N1B4_320 - 3-oxoacyl-[acyl-carrier-protein] synthase, KASII (EC 2.3.1.179), at 376,354 to 377,628
_320
Position (kb)
375
376
377 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 374.662 kb on + strand at 374.716 kb on + strand at 374.717 kb on - strand at 374.764 kb on - strand at 374.764 kb on - strand at 374.764 kb on - strand at 374.810 kb on - strand at 374.811 kb on + strand at 375.000 kb on + strand at 375.000 kb on + strand, within Pf1N1B4_318 at 375.036 kb on + strand, within Pf1N1B4_318 at 375.036 kb on + strand, within Pf1N1B4_318 at 375.036 kb on + strand, within Pf1N1B4_318 at 375.037 kb on - strand at 375.037 kb on - strand, within Pf1N1B4_318 at 375.037 kb on - strand, within Pf1N1B4_318 at 375.037 kb on - strand at 375.037 kb on - strand, within Pf1N1B4_318 at 375.037 kb on - strand at 375.037 kb on - strand, within Pf1N1B4_318 at 375.037 kb on - strand, within Pf1N1B4_318 at 375.037 kb on - strand, within Pf1N1B4_318 at 375.037 kb on - strand, within Pf1N1B4_318 at 375.186 kb on + strand, within Pf1N1B4_318 at 375.186 kb on + strand, within Pf1N1B4_318 at 375.186 kb on + strand at 375.186 kb on + strand, within Pf1N1B4_318 at 375.186 kb on + strand at 375.187 kb on - strand, within Pf1N1B4_318 at 375.187 kb on - strand, within Pf1N1B4_318 at 375.543 kb on + strand at 375.609 kb on + strand at 375.609 kb on + strand at 375.609 kb on + strand at 375.609 kb on + strand at 375.609 kb on + strand at 375.609 kb on + strand at 375.609 kb on + strand at 375.609 kb on + strand at 375.609 kb on + strand at 375.610 kb on - strand at 375.610 kb on - strand at 375.687 kb on + strand at 375.687 kb on + strand at 375.688 kb on - strand at 375.688 kb on - strand at 375.688 kb on - strand at 375.743 kb on + strand, within Pf1N1B4_319 at 375.743 kb on + strand, within Pf1N1B4_319 at 375.743 kb on + strand, within Pf1N1B4_319 at 375.743 kb on + strand, within Pf1N1B4_319 at 375.743 kb on + strand, within Pf1N1B4_319 at 375.743 kb on + strand, within Pf1N1B4_319 at 375.744 kb on - strand, within Pf1N1B4_319 at 375.744 kb on - strand, within Pf1N1B4_319 at 375.744 kb on - strand, within Pf1N1B4_319 at 375.744 kb on - strand, within Pf1N1B4_319 at 375.744 kb on - strand, within Pf1N1B4_319 at 375.744 kb on - strand at 375.744 kb on - strand, within Pf1N1B4_319 at 376.084 kb on + strand, within Pf1N1B4_319 at 376.102 kb on - strand, within Pf1N1B4_319 at 376.102 kb on - strand, within Pf1N1B4_319 at 376.102 kb on - strand, within Pf1N1B4_319 at 376.168 kb on - strand, within Pf1N1B4_319 at 376.168 kb on - strand, within Pf1N1B4_319 at 376.168 kb on - strand, within Pf1N1B4_319 at 376.283 kb on - strand at 376.325 kb on + strand at 376.326 kb on - strand at 376.332 kb on + strand at 376.333 kb on - strand at 376.333 kb on - strand at 376.346 kb on - strand at 376.346 kb on - strand at 376.346 kb on - strand at 376.346 kb on - strand at 376.356 kb on - strand at 376.356 kb on - strand at 376.356 kb on - strand at 376.356 kb on - strand at 376.368 kb on - strand at 376.368 kb on - strand at 376.424 kb on + strand at 376.491 kb on + strand, within Pf1N1B4_320 at 376.500 kb on + strand at 376.501 kb on - strand, within Pf1N1B4_320 at 376.797 kb on - strand, within Pf1N1B4_320 at 376.797 kb on - strand, within Pf1N1B4_320 at 376.797 kb on - strand at 376.965 kb on - strand at 377.049 kb on + strand, within Pf1N1B4_320 at 377.049 kb on + strand, within Pf1N1B4_320 at 377.050 kb on - strand, within Pf1N1B4_320 at 377.050 kb on - strand, within Pf1N1B4_320
Per-strain Table
Position Strand Gene LocusTag Fraction LB 37C remove 374,662 + +1.1 374,716 + +0.7 374,717 - -2.2 374,764 - +0.5 374,764 - +1.8 374,764 - -0.4 374,810 - -0.3 374,811 + -1.1 375,000 + +0.5 375,000 + Pf1N1B4_318 0.28 +0.8 375,036 + Pf1N1B4_318 0.32 -1.8 375,036 + Pf1N1B4_318 0.32 +0.3 375,036 + Pf1N1B4_318 0.32 -1.3 375,037 - +0.9 375,037 - Pf1N1B4_318 0.32 -2.7 375,037 - Pf1N1B4_318 0.32 -0.9 375,037 - -0.3 375,037 - Pf1N1B4_318 0.32 -0.6 375,037 - -0.5 375,037 - Pf1N1B4_318 0.32 +0.2 375,037 - Pf1N1B4_318 0.32 -0.7 375,037 - Pf1N1B4_318 0.32 -0.5 375,037 - Pf1N1B4_318 0.32 +0.4 375,186 + Pf1N1B4_318 0.50 +1.1 375,186 + Pf1N1B4_318 0.50 -2.7 375,186 + -1.1 375,186 + Pf1N1B4_318 0.50 +0.1 375,186 + -0.5 375,187 - Pf1N1B4_318 0.50 -3.1 375,187 - Pf1N1B4_318 0.50 +1.7 375,543 + -1.4 375,609 + -0.2 375,609 + +1.7 375,609 + +0.3 375,609 + +0.7 375,609 + -1.1 375,609 + +2.7 375,609 + +0.4 375,609 + +0.7 375,609 + -1.2 375,610 - -2.0 375,610 - -2.2 375,687 + +3.6 375,687 + -1.7 375,688 - +1.4 375,688 - +0.4 375,688 - +2.5 375,743 + Pf1N1B4_319 0.17 -3.9 375,743 + Pf1N1B4_319 0.17 -1.1 375,743 + Pf1N1B4_319 0.17 -2.3 375,743 + Pf1N1B4_319 0.17 -1.7 375,743 + Pf1N1B4_319 0.17 -3.4 375,743 + Pf1N1B4_319 0.17 +0.2 375,744 - Pf1N1B4_319 0.17 -2.9 375,744 - Pf1N1B4_319 0.17 -1.1 375,744 - Pf1N1B4_319 0.17 -1.7 375,744 - Pf1N1B4_319 0.17 -0.6 375,744 - Pf1N1B4_319 0.17 +1.1 375,744 - -0.3 375,744 - Pf1N1B4_319 0.17 -1.5 376,084 + Pf1N1B4_319 0.67 -1.9 376,102 - Pf1N1B4_319 0.70 -1.0 376,102 - Pf1N1B4_319 0.70 -1.3 376,102 - Pf1N1B4_319 0.70 -1.7 376,168 - Pf1N1B4_319 0.79 -1.3 376,168 - Pf1N1B4_319 0.79 -1.3 376,168 - Pf1N1B4_319 0.79 -1.7 376,283 - -0.1 376,325 + -0.9 376,326 - +0.5 376,332 + -0.7 376,333 - -0.5 376,333 - -2.2 376,346 - -0.1 376,346 - -0.1 376,346 - -1.9 376,346 - +0.6 376,356 - -0.1 376,356 - -1.9 376,356 - -0.9 376,356 - +0.1 376,368 - +0.9 376,368 - -1.4 376,424 + -1.1 376,491 + Pf1N1B4_320 0.11 -0.0 376,500 + +0.5 376,501 - Pf1N1B4_320 0.12 -0.5 376,797 - Pf1N1B4_320 0.35 -1.9 376,797 - Pf1N1B4_320 0.35 -0.5 376,797 - -0.1 376,965 - -0.1 377,049 + Pf1N1B4_320 0.55 +1.2 377,049 + Pf1N1B4_320 0.55 -1.3 377,050 - Pf1N1B4_320 0.55 +2.7 377,050 - Pf1N1B4_320 0.55 -1.2
Or see this region's nucleotide sequence