Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3185

Experiment: LB 37C

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3183 and Pf1N1B4_3184 are separated by 145 nucleotidesPf1N1B4_3184 and Pf1N1B4_3185 are separated by 199 nucleotidesPf1N1B4_3185 and Pf1N1B4_3186 are separated by 5 nucleotidesPf1N1B4_3186 and Pf1N1B4_3187 are separated by 114 nucleotides Pf1N1B4_3183 - Glutathione S-transferase (EC 2.5.1.18), at 3,567,569 to 3,568,213 _3183 Pf1N1B4_3184 - Isochorismatase (EC 3.3.2.1), at 3,568,359 to 3,568,916 _3184 Pf1N1B4_3185 - Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1), at 3,569,116 to 3,569,325 _3185 Pf1N1B4_3186 - Conserved secreted protein, at 3,569,331 to 3,569,816 _3186 Pf1N1B4_3187 - UPF0225 protein YchJ, at 3,569,931 to 3,570,407 _3187 Position (kb) 3569 3570Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3568.137 kb on + strand, within Pf1N1B4_3183at 3568.138 kb on - strand, within Pf1N1B4_3183at 3568.197 kb on + strandat 3568.198 kb on - strandat 3568.200 kb on + strandat 3568.200 kb on + strandat 3568.201 kb on - strandat 3568.201 kb on - strandat 3568.201 kb on - strandat 3568.201 kb on - strandat 3568.201 kb on - strandat 3568.201 kb on - strandat 3568.201 kb on - strandat 3568.245 kb on + strandat 3568.302 kb on - strandat 3568.787 kb on + strand, within Pf1N1B4_3184at 3568.787 kb on + strand, within Pf1N1B4_3184at 3568.788 kb on - strand, within Pf1N1B4_3184at 3568.806 kb on + strand, within Pf1N1B4_3184at 3568.807 kb on - strand, within Pf1N1B4_3184at 3568.872 kb on + strandat 3568.872 kb on + strandat 3568.914 kb on + strandat 3568.954 kb on + strandat 3568.954 kb on + strandat 3569.013 kb on + strandat 3569.013 kb on + strandat 3569.025 kb on + strandat 3569.344 kb on - strandat 3569.358 kb on + strandat 3569.386 kb on + strand, within Pf1N1B4_3186at 3569.386 kb on + strand, within Pf1N1B4_3186at 3569.386 kb on + strand, within Pf1N1B4_3186at 3569.387 kb on - strand, within Pf1N1B4_3186at 3569.387 kb on - strand, within Pf1N1B4_3186at 3569.387 kb on - strandat 3569.387 kb on - strand, within Pf1N1B4_3186at 3569.387 kb on - strand, within Pf1N1B4_3186at 3569.401 kb on - strand, within Pf1N1B4_3186at 3569.401 kb on - strand, within Pf1N1B4_3186at 3569.513 kb on + strand, within Pf1N1B4_3186at 3569.513 kb on + strand, within Pf1N1B4_3186at 3569.514 kb on - strand, within Pf1N1B4_3186at 3569.519 kb on - strand, within Pf1N1B4_3186at 3569.577 kb on + strand, within Pf1N1B4_3186at 3569.577 kb on + strand, within Pf1N1B4_3186at 3569.577 kb on + strand, within Pf1N1B4_3186at 3569.577 kb on + strand, within Pf1N1B4_3186at 3569.577 kb on + strand, within Pf1N1B4_3186at 3569.577 kb on + strand, within Pf1N1B4_3186at 3569.577 kb on + strand, within Pf1N1B4_3186at 3569.577 kb on + strand, within Pf1N1B4_3186at 3569.577 kb on + strand, within Pf1N1B4_3186at 3569.577 kb on + strand, within Pf1N1B4_3186at 3569.577 kb on + strand, within Pf1N1B4_3186at 3569.577 kb on + strand, within Pf1N1B4_3186at 3569.577 kb on + strand, within Pf1N1B4_3186at 3569.577 kb on + strand, within Pf1N1B4_3186at 3569.577 kb on + strand, within Pf1N1B4_3186at 3569.578 kb on - strand, within Pf1N1B4_3186at 3569.578 kb on - strand, within Pf1N1B4_3186at 3569.578 kb on - strand, within Pf1N1B4_3186at 3569.578 kb on - strand, within Pf1N1B4_3186at 3569.578 kb on - strand, within Pf1N1B4_3186at 3569.578 kb on - strand, within Pf1N1B4_3186at 3569.578 kb on - strand, within Pf1N1B4_3186at 3569.578 kb on - strand, within Pf1N1B4_3186at 3569.676 kb on + strand, within Pf1N1B4_3186at 3569.676 kb on + strand, within Pf1N1B4_3186at 3569.793 kb on + strandat 3569.793 kb on + strandat 3569.793 kb on + strandat 3569.793 kb on + strandat 3569.794 kb on - strandat 3569.824 kb on + strandat 3569.825 kb on - strandat 3569.845 kb on + strandat 3569.845 kb on + strandat 3569.845 kb on + strandat 3569.879 kb on + strandat 3569.879 kb on + strandat 3569.880 kb on - strandat 3569.944 kb on + strandat 3570.000 kb on - strand, within Pf1N1B4_3187at 3570.000 kb on - strand, within Pf1N1B4_3187at 3570.000 kb on - strand, within Pf1N1B4_3187at 3570.037 kb on + strand, within Pf1N1B4_3187at 3570.037 kb on + strand, within Pf1N1B4_3187at 3570.038 kb on - strand, within Pf1N1B4_3187at 3570.038 kb on - strand, within Pf1N1B4_3187at 3570.058 kb on - strand, within Pf1N1B4_3187at 3570.261 kb on + strand, within Pf1N1B4_3187at 3570.261 kb on + strand, within Pf1N1B4_3187at 3570.261 kb on + strand, within Pf1N1B4_3187at 3570.261 kb on + strand, within Pf1N1B4_3187at 3570.262 kb on - strand, within Pf1N1B4_3187at 3570.262 kb on - strand, within Pf1N1B4_3187at 3570.262 kb on - strand, within Pf1N1B4_3187at 3570.262 kb on - strand, within Pf1N1B4_3187at 3570.265 kb on + strand, within Pf1N1B4_3187at 3570.266 kb on - strand, within Pf1N1B4_3187at 3570.282 kb on + strand, within Pf1N1B4_3187at 3570.282 kb on + strand, within Pf1N1B4_3187at 3570.282 kb on + strand, within Pf1N1B4_3187at 3570.282 kb on + strand, within Pf1N1B4_3187at 3570.282 kb on + strand, within Pf1N1B4_3187at 3570.282 kb on + strand, within Pf1N1B4_3187at 3570.282 kb on + strand, within Pf1N1B4_3187at 3570.283 kb on - strand, within Pf1N1B4_3187at 3570.283 kb on - strandat 3570.283 kb on - strand, within Pf1N1B4_3187at 3570.283 kb on - strand, within Pf1N1B4_3187at 3570.283 kb on - strand, within Pf1N1B4_3187at 3570.283 kb on - strand, within Pf1N1B4_3187at 3570.283 kb on - strand, within Pf1N1B4_3187at 3570.283 kb on - strand, within Pf1N1B4_3187at 3570.283 kb on - strand, within Pf1N1B4_3187at 3570.286 kb on + strand, within Pf1N1B4_3187at 3570.286 kb on + strand, within Pf1N1B4_3187at 3570.287 kb on - strand, within Pf1N1B4_3187at 3570.287 kb on - strand, within Pf1N1B4_3187at 3570.287 kb on - strand, within Pf1N1B4_3187at 3570.287 kb on - strand, within Pf1N1B4_3187

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Per-strain Table

Position Strand Gene LocusTag Fraction LB 37C
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3,568,137 + Pf1N1B4_3183 0.88 +0.3
3,568,138 - Pf1N1B4_3183 0.88 +0.6
3,568,197 + -1.5
3,568,198 - -0.3
3,568,200 + +0.9
3,568,200 + -1.9
3,568,201 - -0.1
3,568,201 - +1.0
3,568,201 - -1.6
3,568,201 - -0.6
3,568,201 - +0.2
3,568,201 - -2.3
3,568,201 - +1.2
3,568,245 + -0.6
3,568,302 - +1.2
3,568,787 + Pf1N1B4_3184 0.77 -0.4
3,568,787 + Pf1N1B4_3184 0.77 -2.0
3,568,788 - Pf1N1B4_3184 0.77 +0.6
3,568,806 + Pf1N1B4_3184 0.80 -2.1
3,568,807 - Pf1N1B4_3184 0.80 -0.2
3,568,872 + -0.8
3,568,872 + -1.3
3,568,914 + -1.6
3,568,954 + -0.6
3,568,954 + -0.7
3,569,013 + -0.3
3,569,013 + -1.3
3,569,025 + -2.0
3,569,344 - +0.2
3,569,358 + -1.7
3,569,386 + Pf1N1B4_3186 0.11 -0.1
3,569,386 + Pf1N1B4_3186 0.11 -1.2
3,569,386 + Pf1N1B4_3186 0.11 -2.1
3,569,387 - Pf1N1B4_3186 0.12 +2.0
3,569,387 - Pf1N1B4_3186 0.12 +1.4
3,569,387 - +0.4
3,569,387 - Pf1N1B4_3186 0.12 +0.7
3,569,387 - Pf1N1B4_3186 0.12 +0.0
3,569,401 - Pf1N1B4_3186 0.14 +1.0
3,569,401 - Pf1N1B4_3186 0.14 +1.7
3,569,513 + Pf1N1B4_3186 0.37 -0.7
3,569,513 + Pf1N1B4_3186 0.37 -0.6
3,569,514 - Pf1N1B4_3186 0.38 -1.8
3,569,519 - Pf1N1B4_3186 0.39 +0.4
3,569,577 + Pf1N1B4_3186 0.51 -1.3
3,569,577 + Pf1N1B4_3186 0.51 -1.0
3,569,577 + Pf1N1B4_3186 0.51 +0.4
3,569,577 + Pf1N1B4_3186 0.51 -3.5
3,569,577 + Pf1N1B4_3186 0.51 -0.1
3,569,577 + Pf1N1B4_3186 0.51 -1.4
3,569,577 + Pf1N1B4_3186 0.51 +1.0
3,569,577 + Pf1N1B4_3186 0.51 -1.3
3,569,577 + Pf1N1B4_3186 0.51 -1.1
3,569,577 + Pf1N1B4_3186 0.51 -1.9
3,569,577 + Pf1N1B4_3186 0.51 +1.3
3,569,577 + Pf1N1B4_3186 0.51 +1.2
3,569,577 + Pf1N1B4_3186 0.51 +0.0
3,569,577 + Pf1N1B4_3186 0.51 -2.6
3,569,577 + Pf1N1B4_3186 0.51 -0.2
3,569,578 - Pf1N1B4_3186 0.51 +0.2
3,569,578 - Pf1N1B4_3186 0.51 +1.0
3,569,578 - Pf1N1B4_3186 0.51 +0.4
3,569,578 - Pf1N1B4_3186 0.51 -0.6
3,569,578 - Pf1N1B4_3186 0.51 -0.7
3,569,578 - Pf1N1B4_3186 0.51 +0.3
3,569,578 - Pf1N1B4_3186 0.51 +1.0
3,569,578 - Pf1N1B4_3186 0.51 +0.3
3,569,676 + Pf1N1B4_3186 0.71 +0.4
3,569,676 + Pf1N1B4_3186 0.71 +1.0
3,569,793 + +0.8
3,569,793 + -0.3
3,569,793 + -1.3
3,569,793 + -1.1
3,569,794 - +0.8
3,569,824 + -1.5
3,569,825 - +1.7
3,569,845 + +0.2
3,569,845 + -0.3
3,569,845 + +1.0
3,569,879 + +1.0
3,569,879 + +0.3
3,569,880 - -1.7
3,569,944 + -1.6
3,570,000 - Pf1N1B4_3187 0.14 +0.9
3,570,000 - Pf1N1B4_3187 0.14 +0.2
3,570,000 - Pf1N1B4_3187 0.14 -2.1
3,570,037 + Pf1N1B4_3187 0.22 -1.4
3,570,037 + Pf1N1B4_3187 0.22 +0.6
3,570,038 - Pf1N1B4_3187 0.22 +0.2
3,570,038 - Pf1N1B4_3187 0.22 -0.6
3,570,058 - Pf1N1B4_3187 0.27 -2.0
3,570,261 + Pf1N1B4_3187 0.69 -0.9
3,570,261 + Pf1N1B4_3187 0.69 -1.7
3,570,261 + Pf1N1B4_3187 0.69 -2.3
3,570,261 + Pf1N1B4_3187 0.69 -1.1
3,570,262 - Pf1N1B4_3187 0.69 +2.1
3,570,262 - Pf1N1B4_3187 0.69 -3.2
3,570,262 - Pf1N1B4_3187 0.69 -1.5
3,570,262 - Pf1N1B4_3187 0.69 +1.4
3,570,265 + Pf1N1B4_3187 0.70 +0.7
3,570,266 - Pf1N1B4_3187 0.70 -0.5
3,570,282 + Pf1N1B4_3187 0.74 -1.7
3,570,282 + Pf1N1B4_3187 0.74 -0.6
3,570,282 + Pf1N1B4_3187 0.74 +1.7
3,570,282 + Pf1N1B4_3187 0.74 -2.1
3,570,282 + Pf1N1B4_3187 0.74 +0.7
3,570,282 + Pf1N1B4_3187 0.74 +2.7
3,570,282 + Pf1N1B4_3187 0.74 -1.7
3,570,283 - Pf1N1B4_3187 0.74 -1.6
3,570,283 - +0.2
3,570,283 - Pf1N1B4_3187 0.74 -1.3
3,570,283 - Pf1N1B4_3187 0.74 -2.3
3,570,283 - Pf1N1B4_3187 0.74 -1.3
3,570,283 - Pf1N1B4_3187 0.74 +0.2
3,570,283 - Pf1N1B4_3187 0.74 -3.6
3,570,283 - Pf1N1B4_3187 0.74 +0.4
3,570,283 - Pf1N1B4_3187 0.74 +1.3
3,570,286 + Pf1N1B4_3187 0.74 +0.3
3,570,286 + Pf1N1B4_3187 0.74 -0.6
3,570,287 - Pf1N1B4_3187 0.75 -0.3
3,570,287 - Pf1N1B4_3187 0.75 -1.7
3,570,287 - Pf1N1B4_3187 0.75 -1.2
3,570,287 - Pf1N1B4_3187 0.75 -0.4

Or see this region's nucleotide sequence