Experiment: LB 37C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_28 and Pf1N1B4_29 overlap by 4 nucleotides Pf1N1B4_29 and Pf1N1B4_5164 are separated by 225 nucleotides Pf1N1B4_5164 and Pf1N1B4_30 are separated by 102 nucleotides Pf1N1B4_30 and Pf1N1B4_31 overlap by 4 nucleotides
Pf1N1B4_28 - FIG00958186: hypothetical protein, at 31,985 to 32,185
_28
Pf1N1B4_29 - FIG00967200: hypothetical protein, at 32,182 to 32,412
_29
Pf1N1B4_5164 - tRNA-Asn-GTT, at 32,638 to 32,710
_5164
Pf1N1B4_30 - Cation efflux system protein, at 32,813 to 35,899
_30
Pf1N1B4_31 - Probable Co/Zn/Cd efflux system membrane fusion protein, at 35,896 to 37,017
_31
Position (kb)
32
33
34
35
36 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 31.892 kb on - strand at 31.952 kb on + strand at 31.952 kb on + strand at 31.958 kb on + strand at 31.958 kb on + strand at 31.959 kb on - strand at 31.959 kb on - strand at 31.959 kb on - strand at 31.959 kb on - strand at 31.959 kb on - strand at 32.001 kb on + strand at 32.002 kb on - strand at 32.144 kb on + strand, within Pf1N1B4_28 at 32.170 kb on + strand at 32.171 kb on - strand at 32.171 kb on - strand at 32.223 kb on - strand, within Pf1N1B4_29 at 32.223 kb on - strand, within Pf1N1B4_29 at 32.232 kb on - strand, within Pf1N1B4_29 at 32.232 kb on - strand, within Pf1N1B4_29 at 32.232 kb on - strand, within Pf1N1B4_29 at 32.463 kb on + strand at 32.517 kb on + strand at 32.526 kb on - strand at 32.811 kb on + strand at 32.844 kb on + strand at 32.845 kb on - strand at 32.880 kb on + strand at 32.880 kb on + strand at 32.880 kb on + strand at 32.881 kb on - strand at 32.881 kb on - strand at 32.881 kb on - strand at 32.943 kb on + strand at 32.944 kb on - strand at 33.271 kb on + strand at 33.272 kb on - strand at 33.275 kb on - strand at 33.275 kb on - strand, within Pf1N1B4_30 at 33.639 kb on - strand, within Pf1N1B4_30 at 33.639 kb on - strand, within Pf1N1B4_30 at 33.655 kb on + strand, within Pf1N1B4_30 at 33.916 kb on - strand, within Pf1N1B4_30 at 34.061 kb on + strand, within Pf1N1B4_30 at 34.083 kb on + strand, within Pf1N1B4_30 at 34.084 kb on - strand, within Pf1N1B4_30 at 34.084 kb on - strand, within Pf1N1B4_30 at 34.110 kb on + strand, within Pf1N1B4_30 at 34.111 kb on - strand, within Pf1N1B4_30 at 34.187 kb on + strand, within Pf1N1B4_30 at 34.188 kb on - strand at 34.188 kb on - strand, within Pf1N1B4_30 at 34.188 kb on - strand, within Pf1N1B4_30 at 34.190 kb on - strand, within Pf1N1B4_30 at 34.368 kb on + strand, within Pf1N1B4_30 at 34.368 kb on + strand, within Pf1N1B4_30 at 34.369 kb on - strand, within Pf1N1B4_30 at 34.384 kb on - strand, within Pf1N1B4_30 at 34.451 kb on - strand, within Pf1N1B4_30 at 34.451 kb on - strand, within Pf1N1B4_30 at 34.454 kb on + strand, within Pf1N1B4_30 at 34.455 kb on - strand at 34.455 kb on - strand, within Pf1N1B4_30 at 34.455 kb on - strand at 34.518 kb on + strand, within Pf1N1B4_30 at 34.556 kb on - strand, within Pf1N1B4_30 at 34.733 kb on - strand at 34.733 kb on - strand, within Pf1N1B4_30 at 34.913 kb on - strand, within Pf1N1B4_30 at 34.913 kb on - strand, within Pf1N1B4_30 at 35.033 kb on - strand, within Pf1N1B4_30 at 35.164 kb on + strand, within Pf1N1B4_30 at 35.165 kb on - strand, within Pf1N1B4_30 at 35.320 kb on - strand, within Pf1N1B4_30 at 35.463 kb on + strand, within Pf1N1B4_30 at 35.600 kb on - strand at 35.610 kb on + strand at 35.611 kb on - strand at 35.611 kb on - strand at 35.662 kb on - strand at 35.699 kb on + strand at 35.812 kb on - strand at 36.245 kb on + strand at 36.245 kb on + strand, within Pf1N1B4_31 at 36.370 kb on + strand, within Pf1N1B4_31 at 36.370 kb on + strand, within Pf1N1B4_31 at 36.479 kb on - strand, within Pf1N1B4_31 at 36.479 kb on - strand, within Pf1N1B4_31 at 36.479 kb on - strand, within Pf1N1B4_31 at 36.479 kb on - strand, within Pf1N1B4_31 at 36.560 kb on - strand, within Pf1N1B4_31 at 36.750 kb on + strand, within Pf1N1B4_31 at 36.751 kb on - strand, within Pf1N1B4_31 at 36.751 kb on - strand, within Pf1N1B4_31
Per-strain Table
Position Strand Gene LocusTag Fraction LB 37C remove 31,892 - +1.7 31,952 + -1.0 31,952 + -1.2 31,958 + +0.6 31,958 + -1.2 31,959 - +0.6 31,959 - +0.4 31,959 - -0.4 31,959 - -1.1 31,959 - -1.8 32,001 + +0.1 32,002 - -0.9 32,144 + Pf1N1B4_28 0.79 +0.7 32,170 + -0.2 32,171 - -2.2 32,171 - -1.6 32,223 - Pf1N1B4_29 0.18 -2.9 32,223 - Pf1N1B4_29 0.18 -0.6 32,232 - Pf1N1B4_29 0.22 -1.6 32,232 - Pf1N1B4_29 0.22 +0.6 32,232 - Pf1N1B4_29 0.22 +2.3 32,463 + -1.6 32,517 + +2.0 32,526 - +1.6 32,811 + +0.9 32,844 + -1.8 32,845 - +1.1 32,880 + +0.7 32,880 + -1.7 32,880 + -1.5 32,881 - -1.1 32,881 - +1.2 32,881 - -1.1 32,943 + -0.5 32,944 - +0.9 33,271 + -0.2 33,272 - +1.6 33,275 - +0.2 33,275 - Pf1N1B4_30 0.15 +0.7 33,639 - Pf1N1B4_30 0.27 -0.3 33,639 - Pf1N1B4_30 0.27 -2.7 33,655 + Pf1N1B4_30 0.27 -1.2 33,916 - Pf1N1B4_30 0.36 -1.4 34,061 + Pf1N1B4_30 0.40 -1.3 34,083 + Pf1N1B4_30 0.41 -0.9 34,084 - Pf1N1B4_30 0.41 +1.4 34,084 - Pf1N1B4_30 0.41 +1.0 34,110 + Pf1N1B4_30 0.42 -2.2 34,111 - Pf1N1B4_30 0.42 +0.5 34,187 + Pf1N1B4_30 0.45 -1.4 34,188 - +0.6 34,188 - Pf1N1B4_30 0.45 -1.1 34,188 - Pf1N1B4_30 0.45 -1.2 34,190 - Pf1N1B4_30 0.45 -0.4 34,368 + Pf1N1B4_30 0.50 -1.2 34,368 + Pf1N1B4_30 0.50 -0.8 34,369 - Pf1N1B4_30 0.50 -1.4 34,384 - Pf1N1B4_30 0.51 -0.8 34,451 - Pf1N1B4_30 0.53 -2.6 34,451 - Pf1N1B4_30 0.53 -1.0 34,454 + Pf1N1B4_30 0.53 -1.7 34,455 - -1.0 34,455 - Pf1N1B4_30 0.53 +2.0 34,455 - +2.6 34,518 + Pf1N1B4_30 0.55 +1.4 34,556 - Pf1N1B4_30 0.56 -0.8 34,733 - -0.0 34,733 - Pf1N1B4_30 0.62 -0.8 34,913 - Pf1N1B4_30 0.68 +2.1 34,913 - Pf1N1B4_30 0.68 +0.2 35,033 - Pf1N1B4_30 0.72 -1.6 35,164 + Pf1N1B4_30 0.76 -2.0 35,165 - Pf1N1B4_30 0.76 +0.9 35,320 - Pf1N1B4_30 0.81 -1.7 35,463 + Pf1N1B4_30 0.86 +1.8 35,600 - -0.2 35,610 + +0.0 35,611 - -1.0 35,611 - +1.1 35,662 - +0.7 35,699 + -1.5 35,812 - -0.6 36,245 + -0.6 36,245 + Pf1N1B4_31 0.31 -0.9 36,370 + Pf1N1B4_31 0.42 -0.2 36,370 + Pf1N1B4_31 0.42 +1.0 36,479 - Pf1N1B4_31 0.52 +1.4 36,479 - Pf1N1B4_31 0.52 -1.2 36,479 - Pf1N1B4_31 0.52 +1.9 36,479 - Pf1N1B4_31 0.52 -1.9 36,560 - Pf1N1B4_31 0.59 +0.1 36,750 + Pf1N1B4_31 0.76 +1.6 36,751 - Pf1N1B4_31 0.76 -2.5 36,751 - Pf1N1B4_31 0.76 -2.7
Or see this region's nucleotide sequence