Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2514

Experiment: LB 37C

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2513 and Pf1N1B4_2514 are separated by 67 nucleotidesPf1N1B4_2514 and Pf1N1B4_2515 are separated by 481 nucleotidesPf1N1B4_2515 and Pf1N1B4_2516 are separated by 199 nucleotides Pf1N1B4_2513 - RNA polymerase sigma factor RpoD, at 2,850,910 to 2,852,757 _2513 Pf1N1B4_2514 - DNA primase (EC 2.7.7.-), at 2,852,825 to 2,854,798 _2514 Pf1N1B4_2515 - SSU ribosomal protein S21p, at 2,855,280 to 2,855,495 _2515 Pf1N1B4_2516 - TsaD/Kae1/Qri7 protein, required for threonylcarbamoyladenosine t(6)A37 formation in tRNA, at 2,855,695 to 2,856,720 _2516 Position (kb) 2852 2853 2854 2855Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2852.810 kb on - strandat 2854.924 kb on - strandat 2854.924 kb on - strandat 2854.924 kb on - strandat 2854.951 kb on - strandat 2854.951 kb on - strandat 2854.951 kb on - strandat 2854.951 kb on - strandat 2854.951 kb on - strandat 2854.961 kb on - strandat 2855.679 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB 37C
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2,852,810 - -0.1
2,854,924 - -1.5
2,854,924 - +0.1
2,854,924 - +1.7
2,854,951 - -1.9
2,854,951 - +2.3
2,854,951 - -1.7
2,854,951 - -1.7
2,854,951 - +3.3
2,854,961 - -1.4
2,855,679 + -0.6

Or see this region's nucleotide sequence