Experiment: LB 37C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_233 and Pf1N1B4_234 are separated by 307 nucleotides Pf1N1B4_234 and Pf1N1B4_235 are separated by 65 nucleotides Pf1N1B4_235 and Pf1N1B4_236 are separated by 22 nucleotides Pf1N1B4_236 and Pf1N1B4_237 are separated by 81 nucleotides
Pf1N1B4_233 - Transcription regulator [contains diacylglycerol kinase catalytic domain], at 293,604 to 294,536
_233
Pf1N1B4_234 - hypothetical protein, at 294,844 to 295,152
_234
Pf1N1B4_235 - Molybdopterin biosynthesis protein MoeA, at 295,218 to 296,405
_235
Pf1N1B4_236 - Molybdenum cofactor biosynthesis protein MoaB, at 296,428 to 296,967
_236
Pf1N1B4_237 - Molybdopterin-guanine dinucleotide biosynthesis protein MobA, at 297,049 to 297,651
_237
Position (kb) 295
296
297 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 294.264 kb on + strand, within Pf1N1B4_233 at 294.265 kb on - strand at 294.351 kb on - strand, within Pf1N1B4_233 at 294.555 kb on + strand at 294.555 kb on + strand at 294.555 kb on + strand at 294.556 kb on - strand at 294.556 kb on - strand at 294.578 kb on + strand at 294.579 kb on - strand at 294.579 kb on - strand at 294.600 kb on + strand at 294.601 kb on - strand at 294.628 kb on + strand at 294.649 kb on - strand at 294.649 kb on - strand at 294.694 kb on + strand at 294.695 kb on - strand at 294.695 kb on - strand at 294.730 kb on + strand at 294.734 kb on + strand at 294.735 kb on - strand at 294.735 kb on - strand at 294.821 kb on + strand at 294.997 kb on + strand at 294.997 kb on + strand, within Pf1N1B4_234 at 294.997 kb on + strand, within Pf1N1B4_234 at 294.997 kb on + strand, within Pf1N1B4_234 at 294.998 kb on - strand, within Pf1N1B4_234 at 294.998 kb on - strand, within Pf1N1B4_234 at 295.150 kb on + strand at 295.150 kb on + strand at 295.150 kb on + strand at 295.216 kb on + strand at 295.217 kb on - strand at 295.275 kb on - strand at 295.348 kb on + strand, within Pf1N1B4_235 at 295.349 kb on - strand, within Pf1N1B4_235 at 295.387 kb on + strand, within Pf1N1B4_235 at 295.388 kb on - strand, within Pf1N1B4_235 at 295.388 kb on - strand, within Pf1N1B4_235 at 295.388 kb on - strand, within Pf1N1B4_235 at 295.483 kb on + strand, within Pf1N1B4_235 at 295.735 kb on - strand, within Pf1N1B4_235 at 295.735 kb on - strand, within Pf1N1B4_235 at 295.735 kb on - strand, within Pf1N1B4_235 at 295.777 kb on - strand, within Pf1N1B4_235 at 295.812 kb on - strand, within Pf1N1B4_235 at 295.819 kb on + strand, within Pf1N1B4_235 at 295.820 kb on - strand, within Pf1N1B4_235 at 295.820 kb on - strand, within Pf1N1B4_235 at 295.820 kb on - strand, within Pf1N1B4_235 at 295.820 kb on - strand, within Pf1N1B4_235 at 295.820 kb on - strand, within Pf1N1B4_235 at 295.949 kb on + strand, within Pf1N1B4_235 at 296.053 kb on - strand, within Pf1N1B4_235 at 296.053 kb on - strand, within Pf1N1B4_235 at 296.148 kb on - strand, within Pf1N1B4_235 at 296.148 kb on - strand, within Pf1N1B4_235 at 296.240 kb on - strand, within Pf1N1B4_235 at 296.638 kb on + strand, within Pf1N1B4_236 at 296.821 kb on + strand, within Pf1N1B4_236 at 296.821 kb on + strand, within Pf1N1B4_236 at 296.821 kb on + strand, within Pf1N1B4_236 at 296.838 kb on + strand, within Pf1N1B4_236 at 296.884 kb on - strand, within Pf1N1B4_236 at 296.947 kb on + strand at 296.948 kb on - strand at 296.948 kb on - strand at 296.967 kb on + strand at 296.967 kb on + strand at 296.967 kb on + strand at 296.968 kb on - strand at 296.968 kb on - strand at 296.983 kb on + strand at 296.983 kb on + strand at 296.983 kb on + strand at 296.984 kb on - strand at 297.003 kb on + strand at 297.003 kb on + strand at 297.003 kb on + strand at 297.003 kb on + strand at 297.003 kb on + strand at 297.003 kb on + strand at 297.003 kb on + strand at 297.003 kb on + strand at 297.003 kb on + strand at 297.004 kb on - strand at 297.004 kb on - strand at 297.005 kb on + strand at 297.005 kb on + strand at 297.005 kb on + strand at 297.006 kb on - strand at 297.045 kb on + strand at 297.045 kb on + strand at 297.045 kb on + strand at 297.045 kb on + strand at 297.045 kb on + strand at 297.046 kb on - strand at 297.046 kb on - strand at 297.060 kb on + strand at 297.060 kb on + strand at 297.061 kb on - strand at 297.061 kb on - strand at 297.061 kb on - strand at 297.238 kb on + strand, within Pf1N1B4_237 at 297.239 kb on - strand, within Pf1N1B4_237 at 297.239 kb on - strand, within Pf1N1B4_237 at 297.239 kb on - strand, within Pf1N1B4_237 at 297.341 kb on + strand, within Pf1N1B4_237 at 297.341 kb on + strand, within Pf1N1B4_237 at 297.341 kb on + strand, within Pf1N1B4_237 at 297.342 kb on - strand, within Pf1N1B4_237
Per-strain Table
Position Strand Gene LocusTag Fraction LB 37C remove 294,264 + Pf1N1B4_233 0.71 +1.8 294,265 - +0.2 294,351 - Pf1N1B4_233 0.80 +0.8 294,555 + -1.9 294,555 + -1.5 294,555 + -0.8 294,556 - -2.7 294,556 - +0.3 294,578 + -0.8 294,579 - +0.9 294,579 - -1.3 294,600 + +1.5 294,601 - +0.5 294,628 + +0.4 294,649 - -0.3 294,649 - -0.3 294,694 + -1.8 294,695 - +1.7 294,695 - -1.0 294,730 + +3.5 294,734 + -1.3 294,735 - -2.7 294,735 - -0.3 294,821 + -0.6 294,997 + -0.0 294,997 + Pf1N1B4_234 0.50 +0.3 294,997 + Pf1N1B4_234 0.50 -1.0 294,997 + Pf1N1B4_234 0.50 -0.4 294,998 - Pf1N1B4_234 0.50 +2.2 294,998 - Pf1N1B4_234 0.50 -1.3 295,150 + -0.7 295,150 + -2.0 295,150 + -1.2 295,216 + -1.2 295,217 - -1.0 295,275 - -0.0 295,348 + Pf1N1B4_235 0.11 -0.9 295,349 - Pf1N1B4_235 0.11 +0.7 295,387 + Pf1N1B4_235 0.14 +1.1 295,388 - Pf1N1B4_235 0.14 +0.3 295,388 - Pf1N1B4_235 0.14 -1.7 295,388 - Pf1N1B4_235 0.14 +1.0 295,483 + Pf1N1B4_235 0.22 -0.8 295,735 - Pf1N1B4_235 0.44 -1.4 295,735 - Pf1N1B4_235 0.44 -1.0 295,735 - Pf1N1B4_235 0.44 -1.0 295,777 - Pf1N1B4_235 0.47 -1.6 295,812 - Pf1N1B4_235 0.50 -0.6 295,819 + Pf1N1B4_235 0.51 -2.0 295,820 - Pf1N1B4_235 0.51 -1.3 295,820 - Pf1N1B4_235 0.51 -1.9 295,820 - Pf1N1B4_235 0.51 +1.3 295,820 - Pf1N1B4_235 0.51 -1.0 295,820 - Pf1N1B4_235 0.51 +0.4 295,949 + Pf1N1B4_235 0.62 -2.0 296,053 - Pf1N1B4_235 0.70 -0.1 296,053 - Pf1N1B4_235 0.70 -1.8 296,148 - Pf1N1B4_235 0.78 +1.3 296,148 - Pf1N1B4_235 0.78 -1.2 296,240 - Pf1N1B4_235 0.86 -1.5 296,638 + Pf1N1B4_236 0.39 -2.0 296,821 + Pf1N1B4_236 0.73 -0.4 296,821 + Pf1N1B4_236 0.73 -1.8 296,821 + Pf1N1B4_236 0.73 +2.9 296,838 + Pf1N1B4_236 0.76 -0.2 296,884 - Pf1N1B4_236 0.84 -1.3 296,947 + -0.6 296,948 - +1.5 296,948 - +2.2 296,967 + -2.6 296,967 + +0.8 296,967 + -2.7 296,968 - -0.9 296,968 - -1.8 296,983 + -0.6 296,983 + +0.1 296,983 + -2.3 296,984 - -2.2 297,003 + +0.2 297,003 + +0.6 297,003 + -1.1 297,003 + +0.9 297,003 + -1.5 297,003 + -1.0 297,003 + -2.8 297,003 + -0.8 297,003 + +1.1 297,004 - -0.3 297,004 - +1.2 297,005 + -1.6 297,005 + +0.5 297,005 + -1.0 297,006 - -1.7 297,045 + -0.6 297,045 + +0.0 297,045 + -0.5 297,045 + -0.6 297,045 + +1.7 297,046 - -0.2 297,046 - +1.1 297,060 + -1.9 297,060 + -0.8 297,061 - +0.5 297,061 - -0.4 297,061 - +1.0 297,238 + Pf1N1B4_237 0.31 +1.3 297,239 - Pf1N1B4_237 0.32 +1.2 297,239 - Pf1N1B4_237 0.32 -2.0 297,239 - Pf1N1B4_237 0.32 +1.5 297,341 + Pf1N1B4_237 0.48 -1.3 297,341 + Pf1N1B4_237 0.48 -1.3 297,341 + Pf1N1B4_237 0.48 +0.7 297,342 - Pf1N1B4_237 0.49 +0.9
Or see this region's nucleotide sequence