Experiment: LB 37C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_17 and Pf1N1B4_18 are separated by 103 nucleotides Pf1N1B4_18 and Pf1N1B4_19 are separated by 120 nucleotides Pf1N1B4_19 and Pf1N1B4_20 are separated by 166 nucleotides Pf1N1B4_20 and Pf1N1B4_21 are separated by 48 nucleotides
Pf1N1B4_17 - Sensory box/GGDEF family protein, at 18,598 to 21,855
_17
Pf1N1B4_18 - 16 kDa heat shock protein A, at 21,959 to 22,405
_18
Pf1N1B4_19 - tRNA-dihydrouridine synthase C (EC 1.-.-.-), at 22,526 to 23,485
_19
Pf1N1B4_20 - FIG002571: 4-hydroxybenzoyl-CoA thioesterase domain protein, at 23,652 to 24,101
_20
Pf1N1B4_21 - Probable hydrolase, at 24,150 to 24,983
_21
Position (kb) 22
23
24 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 21.598 kb on + strand at 21.598 kb on + strand at 21.794 kb on - strand at 21.794 kb on - strand at 21.802 kb on + strand at 21.802 kb on + strand at 21.802 kb on + strand at 21.802 kb on + strand at 21.853 kb on + strand at 21.885 kb on + strand at 21.893 kb on + strand at 21.893 kb on + strand at 22.141 kb on - strand, within Pf1N1B4_18 at 22.141 kb on - strand, within Pf1N1B4_18 at 22.141 kb on - strand, within Pf1N1B4_18 at 22.146 kb on + strand, within Pf1N1B4_18 at 22.147 kb on - strand, within Pf1N1B4_18 at 22.147 kb on - strand, within Pf1N1B4_18 at 22.147 kb on - strand, within Pf1N1B4_18 at 22.147 kb on - strand, within Pf1N1B4_18 at 22.147 kb on - strand, within Pf1N1B4_18 at 22.147 kb on - strand, within Pf1N1B4_18 at 22.147 kb on - strand, within Pf1N1B4_18 at 22.147 kb on - strand, within Pf1N1B4_18 at 22.147 kb on - strand, within Pf1N1B4_18 at 22.147 kb on - strand, within Pf1N1B4_18 at 22.147 kb on - strand, within Pf1N1B4_18 at 22.290 kb on + strand, within Pf1N1B4_18 at 22.291 kb on - strand, within Pf1N1B4_18 at 22.299 kb on + strand, within Pf1N1B4_18 at 22.300 kb on - strand, within Pf1N1B4_18 at 22.300 kb on - strand, within Pf1N1B4_18 at 22.400 kb on - strand at 22.400 kb on - strand at 22.425 kb on + strand at 22.472 kb on - strand at 22.483 kb on - strand at 22.483 kb on - strand at 22.483 kb on - strand at 22.556 kb on + strand at 22.623 kb on + strand, within Pf1N1B4_19 at 22.959 kb on + strand, within Pf1N1B4_19 at 22.959 kb on + strand, within Pf1N1B4_19 at 23.349 kb on + strand, within Pf1N1B4_19 at 23.353 kb on - strand, within Pf1N1B4_19 at 23.353 kb on - strand, within Pf1N1B4_19 at 23.362 kb on + strand, within Pf1N1B4_19 at 23.363 kb on - strand, within Pf1N1B4_19 at 23.363 kb on - strand, within Pf1N1B4_19 at 23.386 kb on + strand, within Pf1N1B4_19 at 23.594 kb on - strand at 23.692 kb on + strand at 23.882 kb on - strand, within Pf1N1B4_20 at 23.914 kb on - strand, within Pf1N1B4_20 at 24.011 kb on - strand, within Pf1N1B4_20 at 24.121 kb on + strand at 24.122 kb on - strand at 24.122 kb on - strand at 24.122 kb on - strand at 24.252 kb on + strand, within Pf1N1B4_21 at 24.253 kb on - strand, within Pf1N1B4_21 at 24.365 kb on - strand, within Pf1N1B4_21 at 24.423 kb on - strand, within Pf1N1B4_21 at 24.423 kb on - strand at 24.458 kb on - strand, within Pf1N1B4_21 at 24.458 kb on - strand, within Pf1N1B4_21 at 24.458 kb on - strand, within Pf1N1B4_21 at 24.458 kb on - strand, within Pf1N1B4_21 at 24.478 kb on - strand, within Pf1N1B4_21
Per-strain Table
Position Strand Gene LocusTag Fraction LB 37C remove 21,598 + -0.8 21,598 + -0.4 21,794 - -1.0 21,794 - +0.1 21,802 + -2.6 21,802 + -0.4 21,802 + +1.0 21,802 + +1.0 21,853 + +1.3 21,885 + -1.9 21,893 + -0.1 21,893 + -2.0 22,141 - Pf1N1B4_18 0.41 -2.2 22,141 - Pf1N1B4_18 0.41 -1.4 22,141 - Pf1N1B4_18 0.41 -0.2 22,146 + Pf1N1B4_18 0.42 -0.0 22,147 - Pf1N1B4_18 0.42 -2.4 22,147 - Pf1N1B4_18 0.42 -1.1 22,147 - Pf1N1B4_18 0.42 -1.1 22,147 - Pf1N1B4_18 0.42 -1.3 22,147 - Pf1N1B4_18 0.42 +1.8 22,147 - Pf1N1B4_18 0.42 +0.1 22,147 - Pf1N1B4_18 0.42 -2.0 22,147 - Pf1N1B4_18 0.42 -1.3 22,147 - Pf1N1B4_18 0.42 -1.0 22,147 - Pf1N1B4_18 0.42 -0.8 22,147 - Pf1N1B4_18 0.42 -0.0 22,290 + Pf1N1B4_18 0.74 -1.3 22,291 - Pf1N1B4_18 0.74 -3.3 22,299 + Pf1N1B4_18 0.76 +1.8 22,300 - Pf1N1B4_18 0.76 -0.9 22,300 - Pf1N1B4_18 0.76 -0.0 22,400 - -1.8 22,400 - -0.8 22,425 + +1.1 22,472 - +0.9 22,483 - -0.4 22,483 - -1.8 22,483 - -1.6 22,556 + +0.3 22,623 + Pf1N1B4_19 0.10 -1.4 22,959 + Pf1N1B4_19 0.45 -0.4 22,959 + Pf1N1B4_19 0.45 -1.1 23,349 + Pf1N1B4_19 0.86 -1.4 23,353 - Pf1N1B4_19 0.86 -2.1 23,353 - Pf1N1B4_19 0.86 -1.7 23,362 + Pf1N1B4_19 0.87 -2.2 23,363 - Pf1N1B4_19 0.87 -1.3 23,363 - Pf1N1B4_19 0.87 -0.7 23,386 + Pf1N1B4_19 0.90 +1.2 23,594 - -1.1 23,692 + -3.3 23,882 - Pf1N1B4_20 0.51 +1.1 23,914 - Pf1N1B4_20 0.58 +1.3 24,011 - Pf1N1B4_20 0.80 +1.6 24,121 + -0.6 24,122 - -0.8 24,122 - +1.9 24,122 - -1.0 24,252 + Pf1N1B4_21 0.12 -2.8 24,253 - Pf1N1B4_21 0.12 -0.9 24,365 - Pf1N1B4_21 0.26 -1.0 24,423 - Pf1N1B4_21 0.33 +0.1 24,423 - +0.6 24,458 - Pf1N1B4_21 0.37 +0.4 24,458 - Pf1N1B4_21 0.37 +0.1 24,458 - Pf1N1B4_21 0.37 +1.0 24,458 - Pf1N1B4_21 0.37 +1.3 24,478 - Pf1N1B4_21 0.39 +0.8
Or see this region's nucleotide sequence