Experiment: LB 37C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1557 and Pf1N1B4_1558 are separated by 59 nucleotides Pf1N1B4_1558 and Pf1N1B4_1559 are separated by 270 nucleotides
Pf1N1B4_1557 - Polyhydroxyalkanoic acid synthase, at 1,796,052 to 1,797,734
_1557
Pf1N1B4_1558 - Transcriptional regulator PhaD, at 1,797,794 to 1,798,414
_1558
Pf1N1B4_1559 - Polyhydroxyalkanoate granule-associated protein PhaF, at 1,798,685 to 1,799,473
_1559
Position (kb)
1797
1798
1799 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1796.859 kb on + strand, within Pf1N1B4_1557 at 1796.860 kb on - strand at 1796.995 kb on + strand, within Pf1N1B4_1557 at 1796.995 kb on + strand, within Pf1N1B4_1557 at 1796.995 kb on + strand, within Pf1N1B4_1557 at 1796.996 kb on - strand, within Pf1N1B4_1557 at 1796.996 kb on - strand, within Pf1N1B4_1557 at 1797.019 kb on - strand, within Pf1N1B4_1557 at 1797.019 kb on - strand, within Pf1N1B4_1557 at 1797.019 kb on - strand, within Pf1N1B4_1557 at 1797.019 kb on - strand, within Pf1N1B4_1557 at 1797.019 kb on - strand, within Pf1N1B4_1557 at 1797.019 kb on - strand, within Pf1N1B4_1557 at 1797.046 kb on + strand, within Pf1N1B4_1557 at 1797.047 kb on - strand, within Pf1N1B4_1557 at 1797.047 kb on - strand, within Pf1N1B4_1557 at 1797.186 kb on + strand, within Pf1N1B4_1557 at 1797.186 kb on + strand, within Pf1N1B4_1557 at 1797.186 kb on + strand, within Pf1N1B4_1557 at 1797.187 kb on - strand, within Pf1N1B4_1557 at 1797.187 kb on - strand, within Pf1N1B4_1557 at 1797.197 kb on + strand, within Pf1N1B4_1557 at 1797.198 kb on - strand, within Pf1N1B4_1557 at 1797.201 kb on + strand, within Pf1N1B4_1557 at 1797.201 kb on + strand, within Pf1N1B4_1557 at 1797.201 kb on + strand, within Pf1N1B4_1557 at 1797.202 kb on - strand, within Pf1N1B4_1557 at 1797.202 kb on - strand, within Pf1N1B4_1557 at 1797.202 kb on - strand, within Pf1N1B4_1557 at 1797.241 kb on - strand, within Pf1N1B4_1557 at 1797.432 kb on + strand, within Pf1N1B4_1557 at 1797.445 kb on + strand, within Pf1N1B4_1557 at 1797.446 kb on - strand, within Pf1N1B4_1557 at 1797.446 kb on - strand, within Pf1N1B4_1557 at 1797.493 kb on + strand, within Pf1N1B4_1557 at 1797.493 kb on + strand, within Pf1N1B4_1557 at 1797.493 kb on + strand, within Pf1N1B4_1557 at 1797.494 kb on - strand, within Pf1N1B4_1557 at 1797.494 kb on - strand, within Pf1N1B4_1557 at 1797.494 kb on - strand, within Pf1N1B4_1557 at 1797.494 kb on - strand, within Pf1N1B4_1557 at 1797.494 kb on - strand, within Pf1N1B4_1557 at 1797.494 kb on - strand, within Pf1N1B4_1557 at 1797.494 kb on - strand, within Pf1N1B4_1557 at 1797.535 kb on - strand, within Pf1N1B4_1557 at 1797.535 kb on - strand, within Pf1N1B4_1557 at 1797.535 kb on - strand, within Pf1N1B4_1557 at 1797.535 kb on - strand, within Pf1N1B4_1557 at 1797.535 kb on - strand, within Pf1N1B4_1557 at 1797.535 kb on - strand, within Pf1N1B4_1557 at 1797.535 kb on - strand, within Pf1N1B4_1557 at 1797.535 kb on - strand, within Pf1N1B4_1557 at 1797.576 kb on + strand at 1797.576 kb on + strand at 1797.579 kb on + strand at 1797.580 kb on - strand at 1797.580 kb on - strand at 1797.580 kb on - strand at 1797.580 kb on - strand at 1797.603 kb on - strand at 1797.687 kb on + strand at 1797.688 kb on - strand at 1797.714 kb on - strand at 1797.717 kb on + strand at 1797.717 kb on + strand at 1797.717 kb on + strand at 1797.717 kb on + strand at 1797.718 kb on - strand at 1797.718 kb on - strand at 1797.718 kb on - strand at 1797.718 kb on - strand at 1797.718 kb on - strand at 1797.718 kb on - strand at 1797.718 kb on - strand at 1797.718 kb on - strand at 1797.740 kb on - strand at 1797.782 kb on + strand at 1797.782 kb on + strand at 1797.782 kb on + strand at 1797.911 kb on + strand, within Pf1N1B4_1558 at 1797.912 kb on - strand, within Pf1N1B4_1558 at 1797.912 kb on - strand at 1797.915 kb on - strand, within Pf1N1B4_1558 at 1798.026 kb on - strand, within Pf1N1B4_1558 at 1798.275 kb on - strand, within Pf1N1B4_1558 at 1798.319 kb on + strand, within Pf1N1B4_1558 at 1798.319 kb on + strand, within Pf1N1B4_1558 at 1798.319 kb on + strand, within Pf1N1B4_1558 at 1798.319 kb on + strand, within Pf1N1B4_1558 at 1798.445 kb on + strand at 1798.445 kb on + strand at 1798.446 kb on - strand at 1798.526 kb on + strand at 1798.526 kb on + strand at 1798.526 kb on + strand at 1798.526 kb on + strand at 1798.782 kb on - strand, within Pf1N1B4_1559 at 1798.904 kb on - strand, within Pf1N1B4_1559 at 1798.939 kb on + strand, within Pf1N1B4_1559 at 1799.339 kb on - strand, within Pf1N1B4_1559 at 1799.385 kb on - strand, within Pf1N1B4_1559 at 1799.413 kb on - strand at 1799.413 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 37C remove 1,796,859 + Pf1N1B4_1557 0.48 -1.5 1,796,860 - +1.0 1,796,995 + Pf1N1B4_1557 0.56 -1.5 1,796,995 + Pf1N1B4_1557 0.56 +1.6 1,796,995 + Pf1N1B4_1557 0.56 +0.0 1,796,996 - Pf1N1B4_1557 0.56 -1.2 1,796,996 - Pf1N1B4_1557 0.56 -0.7 1,797,019 - Pf1N1B4_1557 0.57 +0.5 1,797,019 - Pf1N1B4_1557 0.57 -0.3 1,797,019 - Pf1N1B4_1557 0.57 +0.5 1,797,019 - Pf1N1B4_1557 0.57 -2.4 1,797,019 - Pf1N1B4_1557 0.57 +1.7 1,797,019 - Pf1N1B4_1557 0.57 -1.0 1,797,046 + Pf1N1B4_1557 0.59 -0.8 1,797,047 - Pf1N1B4_1557 0.59 +0.3 1,797,047 - Pf1N1B4_1557 0.59 -2.3 1,797,186 + Pf1N1B4_1557 0.67 +1.0 1,797,186 + Pf1N1B4_1557 0.67 -0.9 1,797,186 + Pf1N1B4_1557 0.67 -1.4 1,797,187 - Pf1N1B4_1557 0.67 +1.7 1,797,187 - Pf1N1B4_1557 0.67 -2.4 1,797,197 + Pf1N1B4_1557 0.68 +1.1 1,797,198 - Pf1N1B4_1557 0.68 -1.8 1,797,201 + Pf1N1B4_1557 0.68 -1.7 1,797,201 + Pf1N1B4_1557 0.68 -0.6 1,797,201 + Pf1N1B4_1557 0.68 -1.0 1,797,202 - Pf1N1B4_1557 0.68 -1.4 1,797,202 - Pf1N1B4_1557 0.68 -1.2 1,797,202 - Pf1N1B4_1557 0.68 -1.2 1,797,241 - Pf1N1B4_1557 0.71 -1.1 1,797,432 + Pf1N1B4_1557 0.82 -1.0 1,797,445 + Pf1N1B4_1557 0.83 -0.2 1,797,446 - Pf1N1B4_1557 0.83 +0.7 1,797,446 - Pf1N1B4_1557 0.83 -1.0 1,797,493 + Pf1N1B4_1557 0.86 -1.2 1,797,493 + Pf1N1B4_1557 0.86 -0.2 1,797,493 + Pf1N1B4_1557 0.86 +0.6 1,797,494 - Pf1N1B4_1557 0.86 +1.3 1,797,494 - Pf1N1B4_1557 0.86 -0.7 1,797,494 - Pf1N1B4_1557 0.86 +1.0 1,797,494 - Pf1N1B4_1557 0.86 +1.7 1,797,494 - Pf1N1B4_1557 0.86 +0.2 1,797,494 - Pf1N1B4_1557 0.86 -0.1 1,797,494 - Pf1N1B4_1557 0.86 +1.5 1,797,535 - Pf1N1B4_1557 0.88 -1.1 1,797,535 - Pf1N1B4_1557 0.88 +2.0 1,797,535 - Pf1N1B4_1557 0.88 -0.4 1,797,535 - Pf1N1B4_1557 0.88 +0.5 1,797,535 - Pf1N1B4_1557 0.88 -0.5 1,797,535 - Pf1N1B4_1557 0.88 +0.9 1,797,535 - Pf1N1B4_1557 0.88 -0.9 1,797,535 - Pf1N1B4_1557 0.88 -2.6 1,797,576 + -0.5 1,797,576 + -1.1 1,797,579 + -1.2 1,797,580 - +0.1 1,797,580 - -0.7 1,797,580 - +0.7 1,797,580 - -1.0 1,797,603 - +0.4 1,797,687 + -0.7 1,797,688 - -2.2 1,797,714 - +1.2 1,797,717 + -1.5 1,797,717 + -0.0 1,797,717 + -0.4 1,797,717 + -1.9 1,797,718 - -1.9 1,797,718 - +0.7 1,797,718 - -1.7 1,797,718 - -2.2 1,797,718 - +0.4 1,797,718 - -1.5 1,797,718 - -1.5 1,797,718 - +1.7 1,797,740 - +0.5 1,797,782 + -1.1 1,797,782 + +0.1 1,797,782 + -2.4 1,797,911 + Pf1N1B4_1558 0.19 +1.1 1,797,912 - Pf1N1B4_1558 0.19 -1.2 1,797,912 - +0.5 1,797,915 - Pf1N1B4_1558 0.19 +0.3 1,798,026 - Pf1N1B4_1558 0.37 +0.0 1,798,275 - Pf1N1B4_1558 0.77 -1.4 1,798,319 + Pf1N1B4_1558 0.85 +0.0 1,798,319 + Pf1N1B4_1558 0.85 -1.4 1,798,319 + Pf1N1B4_1558 0.85 -0.9 1,798,319 + Pf1N1B4_1558 0.85 -0.5 1,798,445 + +0.2 1,798,445 + +1.6 1,798,446 - +2.3 1,798,526 + -1.2 1,798,526 + -1.8 1,798,526 + -0.7 1,798,526 + -1.4 1,798,782 - Pf1N1B4_1559 0.12 -1.0 1,798,904 - Pf1N1B4_1559 0.28 -0.5 1,798,939 + Pf1N1B4_1559 0.32 +2.3 1,799,339 - Pf1N1B4_1559 0.83 -1.4 1,799,385 - Pf1N1B4_1559 0.89 -0.3 1,799,413 - +1.4 1,799,413 - -0.7
Or see this region's nucleotide sequence