Experiment: LB 37C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1419 and Pf1N1B4_1420 are separated by 182 nucleotides Pf1N1B4_1420 and Pf1N1B4_1421 are separated by 107 nucleotides Pf1N1B4_1421 and Pf1N1B4_1422 are separated by 212 nucleotides Pf1N1B4_1422 and Pf1N1B4_1423 are separated by 3 nucleotides
Pf1N1B4_1419 - putative membrane protein, at 1,628,551 to 1,629,162
_1419
Pf1N1B4_1420 - 3'-to-5' oligoribonuclease (orn), at 1,629,345 to 1,629,887
_1420
Pf1N1B4_1421 - Ribosome small subunit-stimulated GTPase EngC, at 1,629,995 to 1,631,026
_1421
Pf1N1B4_1422 - hypothetical protein, at 1,631,239 to 1,631,646
_1422
Pf1N1B4_1423 - Peptidase C14, caspase catalytic subunit p20, at 1,631,650 to 1,632,609
_1423
Position (kb)
1629
1630
1631
1632 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1629.146 kb on + strand at 1629.146 kb on + strand at 1629.146 kb on + strand at 1629.147 kb on - strand at 1629.195 kb on - strand at 1629.202 kb on + strand at 1629.203 kb on - strand at 1629.237 kb on - strand at 1629.237 kb on - strand at 1629.274 kb on + strand at 1629.274 kb on + strand at 1629.274 kb on + strand at 1629.280 kb on + strand at 1629.280 kb on + strand at 1629.280 kb on + strand at 1629.280 kb on + strand at 1629.280 kb on + strand at 1629.281 kb on - strand at 1629.281 kb on - strand at 1629.281 kb on - strand at 1629.906 kb on - strand at 1629.906 kb on - strand at 1629.918 kb on + strand at 1629.944 kb on + strand at 1629.944 kb on + strand at 1629.965 kb on + strand at 1629.965 kb on + strand at 1631.021 kb on + strand at 1631.021 kb on + strand at 1631.022 kb on - strand at 1631.024 kb on + strand at 1631.025 kb on - strand at 1631.025 kb on - strand at 1631.025 kb on - strand at 1631.075 kb on - strand at 1631.075 kb on - strand at 1631.075 kb on - strand at 1631.075 kb on - strand at 1631.075 kb on - strand at 1631.075 kb on - strand at 1631.083 kb on - strand at 1631.083 kb on - strand at 1631.083 kb on - strand at 1631.121 kb on - strand at 1631.144 kb on - strand at 1631.144 kb on - strand at 1631.194 kb on + strand at 1631.195 kb on - strand at 1631.195 kb on - strand at 1631.195 kb on - strand at 1631.195 kb on - strand at 1631.195 kb on - strand at 1631.195 kb on - strand at 1631.195 kb on - strand at 1631.195 kb on - strand at 1631.248 kb on + strand at 1631.248 kb on + strand at 1631.249 kb on - strand at 1631.249 kb on - strand at 1631.249 kb on - strand at 1631.458 kb on + strand, within Pf1N1B4_1422 at 1631.458 kb on + strand, within Pf1N1B4_1422 at 1631.458 kb on + strand, within Pf1N1B4_1422 at 1631.458 kb on + strand, within Pf1N1B4_1422 at 1631.458 kb on + strand, within Pf1N1B4_1422 at 1631.458 kb on + strand, within Pf1N1B4_1422 at 1631.458 kb on + strand, within Pf1N1B4_1422 at 1631.459 kb on - strand, within Pf1N1B4_1422 at 1631.459 kb on - strand, within Pf1N1B4_1422 at 1631.459 kb on - strand, within Pf1N1B4_1422 at 1631.459 kb on - strand, within Pf1N1B4_1422 at 1631.467 kb on + strand, within Pf1N1B4_1422 at 1631.468 kb on - strand, within Pf1N1B4_1422 at 1631.468 kb on - strand, within Pf1N1B4_1422 at 1631.468 kb on - strand, within Pf1N1B4_1422 at 1631.676 kb on + strand at 1631.677 kb on - strand at 1631.679 kb on + strand at 1631.679 kb on + strand at 1631.679 kb on + strand at 1631.679 kb on + strand at 1631.680 kb on - strand at 1631.680 kb on - strand at 1631.683 kb on + strand at 1631.683 kb on + strand at 1631.683 kb on + strand at 1631.683 kb on + strand at 1631.683 kb on + strand at 1631.683 kb on + strand at 1631.683 kb on + strand at 1631.683 kb on + strand at 1631.683 kb on + strand at 1631.683 kb on + strand at 1631.683 kb on + strand at 1631.684 kb on - strand at 1631.684 kb on - strand at 1631.684 kb on - strand at 1631.878 kb on + strand, within Pf1N1B4_1423 at 1631.878 kb on + strand, within Pf1N1B4_1423 at 1631.878 kb on + strand, within Pf1N1B4_1423 at 1631.879 kb on - strand, within Pf1N1B4_1423 at 1631.879 kb on - strand, within Pf1N1B4_1423 at 1631.879 kb on - strand, within Pf1N1B4_1423 at 1631.942 kb on - strand, within Pf1N1B4_1423 at 1631.942 kb on - strand, within Pf1N1B4_1423 at 1631.942 kb on - strand, within Pf1N1B4_1423 at 1632.002 kb on + strand, within Pf1N1B4_1423 at 1632.024 kb on + strand, within Pf1N1B4_1423 at 1632.025 kb on - strand, within Pf1N1B4_1423
Per-strain Table
Position Strand Gene LocusTag Fraction LB 37C remove 1,629,146 + -0.5 1,629,146 + -1.0 1,629,146 + -1.7 1,629,147 - +0.2 1,629,195 - -0.7 1,629,202 + -0.6 1,629,203 - -0.6 1,629,237 - +0.1 1,629,237 - -0.0 1,629,274 + -0.0 1,629,274 + +0.3 1,629,274 + -1.3 1,629,280 + -1.2 1,629,280 + -0.4 1,629,280 + -1.0 1,629,280 + -1.1 1,629,280 + +0.3 1,629,281 - -2.3 1,629,281 - +0.1 1,629,281 - -1.3 1,629,906 - -1.4 1,629,906 - -0.9 1,629,918 + -0.2 1,629,944 + +1.2 1,629,944 + -0.7 1,629,965 + +0.3 1,629,965 + +0.1 1,631,021 + -1.6 1,631,021 + -1.9 1,631,022 - +1.1 1,631,024 + -1.1 1,631,025 - +1.0 1,631,025 - -0.3 1,631,025 - -2.1 1,631,075 - +1.7 1,631,075 - +0.7 1,631,075 - +0.1 1,631,075 - +0.6 1,631,075 - -0.7 1,631,075 - -0.8 1,631,083 - +1.1 1,631,083 - -1.9 1,631,083 - +1.9 1,631,121 - +2.8 1,631,144 - -1.9 1,631,144 - -1.5 1,631,194 + -0.3 1,631,195 - +1.6 1,631,195 - +0.3 1,631,195 - -0.2 1,631,195 - +0.7 1,631,195 - -1.8 1,631,195 - +1.0 1,631,195 - +0.6 1,631,195 - +1.6 1,631,248 + -2.2 1,631,248 + -0.3 1,631,249 - +0.4 1,631,249 - +1.6 1,631,249 - -3.1 1,631,458 + Pf1N1B4_1422 0.54 -1.2 1,631,458 + Pf1N1B4_1422 0.54 +2.1 1,631,458 + Pf1N1B4_1422 0.54 -0.0 1,631,458 + Pf1N1B4_1422 0.54 -1.3 1,631,458 + Pf1N1B4_1422 0.54 -1.2 1,631,458 + Pf1N1B4_1422 0.54 +2.9 1,631,458 + Pf1N1B4_1422 0.54 +1.8 1,631,459 - Pf1N1B4_1422 0.54 -2.2 1,631,459 - Pf1N1B4_1422 0.54 +1.1 1,631,459 - Pf1N1B4_1422 0.54 +1.1 1,631,459 - Pf1N1B4_1422 0.54 +0.7 1,631,467 + Pf1N1B4_1422 0.56 -1.4 1,631,468 - Pf1N1B4_1422 0.56 -0.9 1,631,468 - Pf1N1B4_1422 0.56 -0.3 1,631,468 - Pf1N1B4_1422 0.56 -1.7 1,631,676 + -0.6 1,631,677 - -0.5 1,631,679 + +0.8 1,631,679 + +2.0 1,631,679 + -0.6 1,631,679 + -2.3 1,631,680 - -0.2 1,631,680 - -0.7 1,631,683 + -1.1 1,631,683 + -0.7 1,631,683 + +0.2 1,631,683 + -1.8 1,631,683 + +1.8 1,631,683 + -0.9 1,631,683 + -1.4 1,631,683 + -0.8 1,631,683 + +1.3 1,631,683 + +0.1 1,631,683 + +1.2 1,631,684 - +0.0 1,631,684 - +0.2 1,631,684 - +1.5 1,631,878 + Pf1N1B4_1423 0.24 -1.0 1,631,878 + Pf1N1B4_1423 0.24 -2.4 1,631,878 + Pf1N1B4_1423 0.24 -1.7 1,631,879 - Pf1N1B4_1423 0.24 -2.1 1,631,879 - Pf1N1B4_1423 0.24 +0.4 1,631,879 - Pf1N1B4_1423 0.24 -0.3 1,631,942 - Pf1N1B4_1423 0.30 -1.9 1,631,942 - Pf1N1B4_1423 0.30 -1.2 1,631,942 - Pf1N1B4_1423 0.30 -1.5 1,632,002 + Pf1N1B4_1423 0.37 -0.8 1,632,024 + Pf1N1B4_1423 0.39 +1.4 1,632,025 - Pf1N1B4_1423 0.39 -1.7
Or see this region's nucleotide sequence