Experiment: ALP with nitrite 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2525 and Dsui_2526 are separated by 191 nucleotides Dsui_2526 and Dsui_2527 overlap by 4 nucleotides Dsui_2527 and Dsui_2528 overlap by 4 nucleotides
Dsui_2525: Dsui_2525 - phage-related protein, at 2,674,983 to 2,675,360
_2525
Dsui_2526: Dsui_2526 - site-specific recombinase, DNA invertase Pin, at 2,675,552 to 2,676,145
_2526
Dsui_2527: Dsui_2527 - transposase, TnpA family, at 2,676,142 to 2,679,174
_2527
Dsui_2528: Dsui_2528 - putative signal transduction protein, at 2,679,171 to 2,681,186
_2528
Position (kb)
2676
2677
2678
2679
2680 Strain fitness (log2 ratio)
-2
-1
0
1 at 2675.151 kb on - strand, within Dsui_2525 at 2675.255 kb on - strand, within Dsui_2525 at 2675.342 kb on + strand at 2675.350 kb on - strand at 2675.368 kb on + strand at 2675.452 kb on - strand at 2675.546 kb on - strand at 2675.569 kb on - strand at 2675.590 kb on - strand at 2675.593 kb on + strand at 2675.611 kb on - strand at 2675.631 kb on - strand, within Dsui_2526 at 2675.732 kb on + strand, within Dsui_2526 at 2675.833 kb on + strand, within Dsui_2526 at 2675.921 kb on - strand, within Dsui_2526 at 2676.040 kb on + strand, within Dsui_2526 at 2676.077 kb on + strand, within Dsui_2526 at 2676.092 kb on - strand at 2676.104 kb on - strand at 2676.201 kb on - strand at 2676.247 kb on - strand at 2676.251 kb on - strand at 2676.300 kb on + strand at 2676.335 kb on - strand at 2676.335 kb on - strand at 2676.371 kb on - strand at 2676.391 kb on + strand at 2676.586 kb on - strand, within Dsui_2527 at 2676.586 kb on - strand at 2676.874 kb on + strand, within Dsui_2527 at 2676.933 kb on + strand, within Dsui_2527 at 2676.933 kb on + strand, within Dsui_2527 at 2676.933 kb on + strand, within Dsui_2527 at 2676.941 kb on - strand, within Dsui_2527 at 2676.941 kb on - strand at 2676.941 kb on - strand, within Dsui_2527 at 2676.941 kb on - strand at 2676.941 kb on - strand, within Dsui_2527 at 2677.033 kb on - strand, within Dsui_2527 at 2677.312 kb on - strand, within Dsui_2527 at 2677.392 kb on - strand, within Dsui_2527 at 2677.416 kb on - strand, within Dsui_2527 at 2677.427 kb on - strand, within Dsui_2527 at 2677.491 kb on + strand, within Dsui_2527 at 2677.766 kb on + strand, within Dsui_2527 at 2677.785 kb on - strand, within Dsui_2527 at 2677.798 kb on - strand, within Dsui_2527 at 2677.898 kb on + strand, within Dsui_2527 at 2677.994 kb on + strand, within Dsui_2527 at 2678.022 kb on + strand, within Dsui_2527 at 2678.126 kb on + strand, within Dsui_2527 at 2678.231 kb on + strand, within Dsui_2527 at 2678.388 kb on + strand, within Dsui_2527 at 2678.390 kb on - strand, within Dsui_2527 at 2678.633 kb on + strand, within Dsui_2527 at 2678.697 kb on + strand, within Dsui_2527 at 2678.721 kb on + strand, within Dsui_2527 at 2678.729 kb on - strand, within Dsui_2527 at 2678.826 kb on + strand, within Dsui_2527 at 2678.951 kb on + strand at 2679.373 kb on + strand, within Dsui_2528 at 2679.419 kb on - strand, within Dsui_2528 at 2679.419 kb on - strand, within Dsui_2528 at 2679.484 kb on + strand, within Dsui_2528 at 2679.492 kb on - strand at 2679.492 kb on - strand, within Dsui_2528 at 2679.520 kb on - strand, within Dsui_2528 at 2679.520 kb on - strand at 2679.520 kb on - strand, within Dsui_2528 at 2679.637 kb on + strand, within Dsui_2528 at 2679.752 kb on + strand, within Dsui_2528 at 2679.760 kb on - strand, within Dsui_2528 at 2679.780 kb on - strand, within Dsui_2528 at 2679.825 kb on - strand, within Dsui_2528 at 2679.885 kb on - strand, within Dsui_2528 at 2679.953 kb on + strand, within Dsui_2528 at 2679.953 kb on + strand, within Dsui_2528 at 2680.030 kb on + strand, within Dsui_2528 at 2680.046 kb on + strand, within Dsui_2528 at 2680.139 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with nitrite 0.3 mM remove 2,675,151 - Dsui_2525 0.44 -0.2 2,675,255 - Dsui_2525 0.72 -0.2 2,675,342 + -2.1 2,675,350 - -0.2 2,675,368 + +0.5 2,675,452 - +0.5 2,675,546 - -0.5 2,675,569 - -0.5 2,675,590 - -0.5 2,675,593 + -0.3 2,675,611 - -0.5 2,675,631 - Dsui_2526 0.13 -0.5 2,675,732 + Dsui_2526 0.30 -0.3 2,675,833 + Dsui_2526 0.47 -0.1 2,675,921 - Dsui_2526 0.62 -1.8 2,676,040 + Dsui_2526 0.82 -0.0 2,676,077 + Dsui_2526 0.88 -0.2 2,676,092 - +0.5 2,676,104 - -1.1 2,676,201 - -0.5 2,676,247 - -0.5 2,676,251 - +0.5 2,676,300 + -1.1 2,676,335 - -0.5 2,676,335 - +0.5 2,676,371 - -1.5 2,676,391 + +0.5 2,676,586 - Dsui_2527 0.15 -0.5 2,676,586 - -1.8 2,676,874 + Dsui_2527 0.24 +0.5 2,676,933 + Dsui_2527 0.26 -1.1 2,676,933 + Dsui_2527 0.26 +0.5 2,676,933 + Dsui_2527 0.26 +0.5 2,676,941 - Dsui_2527 0.26 -1.5 2,676,941 - +0.5 2,676,941 - Dsui_2527 0.26 +0.5 2,676,941 - -0.5 2,676,941 - Dsui_2527 0.26 -0.5 2,677,033 - Dsui_2527 0.29 -1.5 2,677,312 - Dsui_2527 0.39 -1.1 2,677,392 - Dsui_2527 0.41 +0.5 2,677,416 - Dsui_2527 0.42 -2.6 2,677,427 - Dsui_2527 0.42 -1.5 2,677,491 + Dsui_2527 0.44 -0.5 2,677,766 + Dsui_2527 0.54 -2.1 2,677,785 - Dsui_2527 0.54 +0.5 2,677,798 - Dsui_2527 0.55 -1.5 2,677,898 + Dsui_2527 0.58 +0.5 2,677,994 + Dsui_2527 0.61 -0.5 2,678,022 + Dsui_2527 0.62 +0.5 2,678,126 + Dsui_2527 0.65 -1.8 2,678,231 + Dsui_2527 0.69 -0.5 2,678,388 + Dsui_2527 0.74 -0.5 2,678,390 - Dsui_2527 0.74 -0.5 2,678,633 + Dsui_2527 0.82 -0.5 2,678,697 + Dsui_2527 0.84 -1.1 2,678,721 + Dsui_2527 0.85 -1.7 2,678,729 - Dsui_2527 0.85 -2.1 2,678,826 + Dsui_2527 0.88 +0.7 2,678,951 + -1.5 2,679,373 + Dsui_2528 0.10 -1.1 2,679,419 - Dsui_2528 0.12 +0.5 2,679,419 - Dsui_2528 0.12 -0.5 2,679,484 + Dsui_2528 0.16 -1.1 2,679,492 - +0.5 2,679,492 - Dsui_2528 0.16 -0.5 2,679,520 - Dsui_2528 0.17 -1.8 2,679,520 - +0.5 2,679,520 - Dsui_2528 0.17 -1.1 2,679,637 + Dsui_2528 0.23 +0.5 2,679,752 + Dsui_2528 0.29 +0.5 2,679,760 - Dsui_2528 0.29 +0.5 2,679,780 - Dsui_2528 0.30 +1.5 2,679,825 - Dsui_2528 0.32 -2.1 2,679,885 - Dsui_2528 0.35 -1.1 2,679,953 + Dsui_2528 0.39 +0.5 2,679,953 + Dsui_2528 0.39 -1.1 2,680,030 + Dsui_2528 0.43 -0.5 2,680,046 + Dsui_2528 0.43 +0.5 2,680,139 - -0.5
Or see this region's nucleotide sequence