Experiment: ALP with nitrite 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1817 and Dsui_1818 are separated by 31 nucleotides Dsui_1818 and Dsui_1819 are separated by 71 nucleotides Dsui_1819 and Dsui_1820 are separated by 268 nucleotides
Dsui_1817: Dsui_1817 - anaerobic dehydrogenase, typically selenocysteine-containing, at 1,913,778 to 1,916,543
_1817
Dsui_1818: Dsui_1818 - anthranilate phosphoribosyltransferase, at 1,916,575 to 1,917,558
_1818
Dsui_1819: Dsui_1819 - 4-oxalocrotonate tautomerase family enzyme, at 1,917,630 to 1,917,830
_1819
Dsui_1820: Dsui_1820 - ABC-type branched-chain amino acid transport system, periplasmic component, at 1,918,099 to 1,919,232
_1820
Position (kb)
1916
1917
1918 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1915.665 kb on + strand at 1915.689 kb on - strand at 1915.755 kb on - strand at 1915.856 kb on - strand at 1915.931 kb on + strand at 1915.934 kb on - strand at 1915.987 kb on + strand at 1916.078 kb on + strand, within Dsui_1817 at 1916.112 kb on + strand, within Dsui_1817 at 1916.277 kb on - strand at 1916.376 kb on - strand at 1916.384 kb on - strand at 1916.397 kb on + strand at 1916.474 kb on + strand at 1916.536 kb on - strand at 1916.591 kb on + strand at 1916.629 kb on + strand at 1916.636 kb on + strand at 1916.732 kb on - strand, within Dsui_1818 at 1916.800 kb on + strand, within Dsui_1818 at 1916.914 kb on + strand, within Dsui_1818 at 1917.059 kb on + strand, within Dsui_1818 at 1917.059 kb on + strand, within Dsui_1818 at 1917.067 kb on - strand, within Dsui_1818 at 1917.094 kb on + strand, within Dsui_1818 at 1917.152 kb on - strand at 1917.152 kb on - strand, within Dsui_1818 at 1917.191 kb on + strand, within Dsui_1818 at 1917.199 kb on - strand at 1917.386 kb on + strand, within Dsui_1818 at 1917.398 kb on + strand, within Dsui_1818 at 1917.418 kb on + strand, within Dsui_1818 at 1917.418 kb on + strand at 1917.510 kb on + strand at 1917.518 kb on - strand at 1917.518 kb on - strand at 1917.536 kb on + strand at 1917.561 kb on - strand at 1917.617 kb on - strand at 1917.644 kb on + strand at 1917.722 kb on - strand, within Dsui_1819 at 1917.807 kb on + strand, within Dsui_1819 at 1917.843 kb on + strand at 1917.846 kb on - strand at 1918.010 kb on + strand at 1918.055 kb on - strand at 1918.236 kb on + strand, within Dsui_1820 at 1918.245 kb on + strand at 1918.345 kb on + strand, within Dsui_1820 at 1918.435 kb on + strand at 1918.435 kb on + strand, within Dsui_1820
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with nitrite 0.3 mM remove 1,915,665 + +0.5 1,915,689 - +0.5 1,915,755 - +0.5 1,915,856 - +0.5 1,915,931 + -0.5 1,915,934 - +0.5 1,915,987 + +0.5 1,916,078 + Dsui_1817 0.83 -1.5 1,916,112 + Dsui_1817 0.84 -0.5 1,916,277 - -2.3 1,916,376 - +0.5 1,916,384 - +0.5 1,916,397 + -1.8 1,916,474 + -2.0 1,916,536 - -0.5 1,916,591 + -0.0 1,916,629 + -0.3 1,916,636 + -0.5 1,916,732 - Dsui_1818 0.16 +0.5 1,916,800 + Dsui_1818 0.23 -1.6 1,916,914 + Dsui_1818 0.34 +0.5 1,917,059 + Dsui_1818 0.49 -2.3 1,917,059 + Dsui_1818 0.49 -0.8 1,917,067 - Dsui_1818 0.50 -1.0 1,917,094 + Dsui_1818 0.53 -1.4 1,917,152 - +0.5 1,917,152 - Dsui_1818 0.59 -0.5 1,917,191 + Dsui_1818 0.63 -0.6 1,917,199 - -0.5 1,917,386 + Dsui_1818 0.82 -1.6 1,917,398 + Dsui_1818 0.84 -0.0 1,917,418 + Dsui_1818 0.86 -1.2 1,917,418 + -1.0 1,917,510 + -0.5 1,917,518 - +0.5 1,917,518 - +0.5 1,917,536 + -1.0 1,917,561 - -1.8 1,917,617 - -2.3 1,917,644 + -1.5 1,917,722 - Dsui_1819 0.46 -1.5 1,917,807 + Dsui_1819 0.88 -2.8 1,917,843 + -1.5 1,917,846 - -0.5 1,918,010 + -2.5 1,918,055 - -1.0 1,918,236 + Dsui_1820 0.12 -1.0 1,918,245 + +0.6 1,918,345 + Dsui_1820 0.22 -1.8 1,918,435 + +0.6 1,918,435 + Dsui_1820 0.30 +0.6
Or see this region's nucleotide sequence