Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5674

Experiment: LB 30C

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5673 and Pf1N1B4_5674 overlap by 4 nucleotidesPf1N1B4_5674 and Pf1N1B4_5675 are separated by 150 nucleotidesPf1N1B4_5675 and Pf1N1B4_5676 are separated by 108 nucleotidesPf1N1B4_5676 and Pf1N1B4_5677 are separated by 218 nucleotides Pf1N1B4_5673 - Sterol desaturase, at 110,665 to 111,807 _5673 Pf1N1B4_5674 - Strictosidine synthase precursor (EC 4.3.3.2), at 111,804 to 112,892 _5674 Pf1N1B4_5675 - hypothetical protein, at 113,043 to 113,303 _5675 Pf1N1B4_5676 - hypothetical protein, at 113,412 to 113,654 _5676 Pf1N1B4_5677 - putative exported protein, at 113,873 to 114,394 _5677 Position (kb) 111 112 113Strain fitness (log2 ratio) -2 -1 0 1 2at 110.805 kb on - strand, within Pf1N1B4_5673at 110.918 kb on - strand, within Pf1N1B4_5673at 110.971 kb on + strand, within Pf1N1B4_5673at 110.972 kb on - strand, within Pf1N1B4_5673at 111.131 kb on - strandat 111.284 kb on - strand, within Pf1N1B4_5673at 111.476 kb on - strand, within Pf1N1B4_5673at 111.616 kb on + strand, within Pf1N1B4_5673at 111.683 kb on + strand, within Pf1N1B4_5673at 111.832 kb on + strandat 111.832 kb on + strandat 111.832 kb on + strandat 111.833 kb on - strandat 111.833 kb on - strandat 111.874 kb on + strandat 111.943 kb on - strand, within Pf1N1B4_5674at 112.064 kb on + strandat 112.614 kb on + strand, within Pf1N1B4_5674at 112.614 kb on + strand, within Pf1N1B4_5674at 112.615 kb on - strandat 112.615 kb on - strand, within Pf1N1B4_5674at 112.663 kb on + strand, within Pf1N1B4_5674at 112.663 kb on + strand, within Pf1N1B4_5674at 112.788 kb on + strandat 112.788 kb on + strandat 112.788 kb on + strandat 112.788 kb on + strandat 112.815 kb on + strandat 112.816 kb on - strandat 112.830 kb on + strandat 112.830 kb on + strandat 112.890 kb on + strandat 112.891 kb on - strandat 112.891 kb on - strandat 113.055 kb on + strandat 113.056 kb on - strandat 113.056 kb on - strandat 113.056 kb on - strandat 113.142 kb on + strand, within Pf1N1B4_5675at 113.143 kb on - strand, within Pf1N1B4_5675at 113.143 kb on - strand, within Pf1N1B4_5675at 113.228 kb on - strand, within Pf1N1B4_5675at 113.356 kb on - strandat 113.360 kb on - strandat 113.406 kb on - strandat 113.551 kb on + strand, within Pf1N1B4_5676at 113.552 kb on - strand, within Pf1N1B4_5676at 113.848 kb on + strandat 113.849 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB 30C
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110,805 - Pf1N1B4_5673 0.12 +0.6
110,918 - Pf1N1B4_5673 0.22 +0.0
110,971 + Pf1N1B4_5673 0.27 -1.0
110,972 - Pf1N1B4_5673 0.27 +0.1
111,131 - +0.5
111,284 - Pf1N1B4_5673 0.54 -0.2
111,476 - Pf1N1B4_5673 0.71 -0.5
111,616 + Pf1N1B4_5673 0.83 -0.1
111,683 + Pf1N1B4_5673 0.89 +0.6
111,832 + +1.7
111,832 + +0.2
111,832 + +0.5
111,833 - -0.1
111,833 - -0.2
111,874 + +0.3
111,943 - Pf1N1B4_5674 0.13 -0.1
112,064 + +0.1
112,614 + Pf1N1B4_5674 0.74 -0.7
112,614 + Pf1N1B4_5674 0.74 -0.4
112,615 - +1.9
112,615 - Pf1N1B4_5674 0.74 -1.4
112,663 + Pf1N1B4_5674 0.79 -0.2
112,663 + Pf1N1B4_5674 0.79 -1.4
112,788 + +0.6
112,788 + -1.1
112,788 + +0.3
112,788 + +0.1
112,815 + +0.7
112,816 - -0.9
112,830 + +0.9
112,830 + +0.8
112,890 + -1.0
112,891 - +1.1
112,891 - +1.1
113,055 + -2.0
113,056 - +0.3
113,056 - +0.1
113,056 - -1.6
113,142 + Pf1N1B4_5675 0.38 -0.5
113,143 - Pf1N1B4_5675 0.38 +0.6
113,143 - Pf1N1B4_5675 0.38 +0.9
113,228 - Pf1N1B4_5675 0.71 -0.2
113,356 - -0.4
113,360 - -0.4
113,406 - -1.3
113,551 + Pf1N1B4_5676 0.57 -0.2
113,552 - Pf1N1B4_5676 0.58 +1.0
113,848 + +0.6
113,849 - +0.6

Or see this region's nucleotide sequence