Experiment: LB 30C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5673 and Pf1N1B4_5674 overlap by 4 nucleotides Pf1N1B4_5674 and Pf1N1B4_5675 are separated by 150 nucleotides Pf1N1B4_5675 and Pf1N1B4_5676 are separated by 108 nucleotides Pf1N1B4_5676 and Pf1N1B4_5677 are separated by 218 nucleotides
Pf1N1B4_5673 - Sterol desaturase, at 110,665 to 111,807
_5673
Pf1N1B4_5674 - Strictosidine synthase precursor (EC 4.3.3.2), at 111,804 to 112,892
_5674
Pf1N1B4_5675 - hypothetical protein, at 113,043 to 113,303
_5675
Pf1N1B4_5676 - hypothetical protein, at 113,412 to 113,654
_5676
Pf1N1B4_5677 - putative exported protein, at 113,873 to 114,394
_5677
Position (kb)
111
112
113 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 110.805 kb on - strand, within Pf1N1B4_5673 at 110.918 kb on - strand, within Pf1N1B4_5673 at 110.971 kb on + strand, within Pf1N1B4_5673 at 110.972 kb on - strand, within Pf1N1B4_5673 at 111.131 kb on - strand at 111.284 kb on - strand, within Pf1N1B4_5673 at 111.476 kb on - strand, within Pf1N1B4_5673 at 111.616 kb on + strand, within Pf1N1B4_5673 at 111.683 kb on + strand, within Pf1N1B4_5673 at 111.832 kb on + strand at 111.832 kb on + strand at 111.832 kb on + strand at 111.833 kb on - strand at 111.833 kb on - strand at 111.874 kb on + strand at 111.943 kb on - strand, within Pf1N1B4_5674 at 112.064 kb on + strand at 112.614 kb on + strand, within Pf1N1B4_5674 at 112.614 kb on + strand, within Pf1N1B4_5674 at 112.615 kb on - strand at 112.615 kb on - strand, within Pf1N1B4_5674 at 112.663 kb on + strand, within Pf1N1B4_5674 at 112.663 kb on + strand, within Pf1N1B4_5674 at 112.788 kb on + strand at 112.788 kb on + strand at 112.788 kb on + strand at 112.788 kb on + strand at 112.815 kb on + strand at 112.816 kb on - strand at 112.830 kb on + strand at 112.830 kb on + strand at 112.890 kb on + strand at 112.891 kb on - strand at 112.891 kb on - strand at 113.055 kb on + strand at 113.056 kb on - strand at 113.056 kb on - strand at 113.056 kb on - strand at 113.142 kb on + strand, within Pf1N1B4_5675 at 113.143 kb on - strand, within Pf1N1B4_5675 at 113.143 kb on - strand, within Pf1N1B4_5675 at 113.228 kb on - strand, within Pf1N1B4_5675 at 113.356 kb on - strand at 113.360 kb on - strand at 113.406 kb on - strand at 113.551 kb on + strand, within Pf1N1B4_5676 at 113.552 kb on - strand, within Pf1N1B4_5676 at 113.848 kb on + strand at 113.849 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 30C remove 110,805 - Pf1N1B4_5673 0.12 +0.6 110,918 - Pf1N1B4_5673 0.22 +0.0 110,971 + Pf1N1B4_5673 0.27 -1.0 110,972 - Pf1N1B4_5673 0.27 +0.1 111,131 - +0.5 111,284 - Pf1N1B4_5673 0.54 -0.2 111,476 - Pf1N1B4_5673 0.71 -0.5 111,616 + Pf1N1B4_5673 0.83 -0.1 111,683 + Pf1N1B4_5673 0.89 +0.6 111,832 + +1.7 111,832 + +0.2 111,832 + +0.5 111,833 - -0.1 111,833 - -0.2 111,874 + +0.3 111,943 - Pf1N1B4_5674 0.13 -0.1 112,064 + +0.1 112,614 + Pf1N1B4_5674 0.74 -0.7 112,614 + Pf1N1B4_5674 0.74 -0.4 112,615 - +1.9 112,615 - Pf1N1B4_5674 0.74 -1.4 112,663 + Pf1N1B4_5674 0.79 -0.2 112,663 + Pf1N1B4_5674 0.79 -1.4 112,788 + +0.6 112,788 + -1.1 112,788 + +0.3 112,788 + +0.1 112,815 + +0.7 112,816 - -0.9 112,830 + +0.9 112,830 + +0.8 112,890 + -1.0 112,891 - +1.1 112,891 - +1.1 113,055 + -2.0 113,056 - +0.3 113,056 - +0.1 113,056 - -1.6 113,142 + Pf1N1B4_5675 0.38 -0.5 113,143 - Pf1N1B4_5675 0.38 +0.6 113,143 - Pf1N1B4_5675 0.38 +0.9 113,228 - Pf1N1B4_5675 0.71 -0.2 113,356 - -0.4 113,360 - -0.4 113,406 - -1.3 113,551 + Pf1N1B4_5676 0.57 -0.2 113,552 - Pf1N1B4_5676 0.58 +1.0 113,848 + +0.6 113,849 - +0.6
Or see this region's nucleotide sequence