Experiment: LB 30C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5103 and Pf1N1B4_5104 are separated by 8 nucleotides Pf1N1B4_5104 and Pf1N1B4_5105 overlap by 4 nucleotides Pf1N1B4_5105 and Pf1N1B4_5106 are separated by 33 nucleotides
Pf1N1B4_5103 - Dipeptide transport system permease protein DppC (TC 3.A.1.5.2), at 5,656,705 to 5,657,559
_5103
Pf1N1B4_5104 - Oligopeptide transport system permease protein OppB (TC 3.A.1.5.1), at 5,657,568 to 5,658,542
_5104
Pf1N1B4_5105 - Peptide ABC transporter, ATP-binding protein, at 5,658,539 to 5,659,510
_5105
Pf1N1B4_5106 - Dipeptide-binding ABC transporter, periplasmic substrate-binding component (TC 3.A.1.5.2); Putative hemin-binding lipoprotein, at 5,659,544 to 5,661,058
_5106
Position (kb) 5658
5659
5660 Strain fitness (log2 ratio) -2
-1
0
1 at 5657.719 kb on + strand, within Pf1N1B4_5104 at 5657.720 kb on - strand, within Pf1N1B4_5104 at 5658.309 kb on + strand, within Pf1N1B4_5104 at 5658.597 kb on + strand at 5659.223 kb on + strand at 5659.224 kb on - strand, within Pf1N1B4_5105 at 5659.224 kb on - strand, within Pf1N1B4_5105 at 5659.224 kb on - strand, within Pf1N1B4_5105 at 5659.224 kb on - strand, within Pf1N1B4_5105 at 5659.509 kb on - strand at 5659.520 kb on + strand at 5659.809 kb on - strand, within Pf1N1B4_5106 at 5660.075 kb on + strand, within Pf1N1B4_5106 at 5660.075 kb on + strand at 5660.397 kb on + strand, within Pf1N1B4_5106 at 5660.474 kb on + strand, within Pf1N1B4_5106 at 5660.480 kb on + strand at 5660.481 kb on - strand, within Pf1N1B4_5106 at 5660.481 kb on - strand, within Pf1N1B4_5106
Per-strain Table
Position Strand Gene LocusTag Fraction LB 30C remove 5,657,719 + Pf1N1B4_5104 0.15 -0.4 5,657,720 - Pf1N1B4_5104 0.16 -0.2 5,658,309 + Pf1N1B4_5104 0.76 -0.4 5,658,597 + +0.5 5,659,223 + +0.9 5,659,224 - Pf1N1B4_5105 0.70 -0.2 5,659,224 - Pf1N1B4_5105 0.70 -0.8 5,659,224 - Pf1N1B4_5105 0.70 -0.6 5,659,224 - Pf1N1B4_5105 0.70 -0.8 5,659,509 - +0.5 5,659,520 + +0.5 5,659,809 - Pf1N1B4_5106 0.17 -1.8 5,660,075 + Pf1N1B4_5106 0.35 +0.3 5,660,075 + +0.5 5,660,397 + Pf1N1B4_5106 0.56 -0.1 5,660,474 + Pf1N1B4_5106 0.61 -1.0 5,660,480 + +1.0 5,660,481 - Pf1N1B4_5106 0.62 -0.8 5,660,481 - Pf1N1B4_5106 0.62 -1.0
Or see this region's nucleotide sequence