Experiment: LB 30C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3793 and Pf1N1B4_3794 overlap by 2 nucleotides Pf1N1B4_3794 and Pf1N1B4_3795 are separated by 477 nucleotides Pf1N1B4_3795 and Pf1N1B4_3796 are separated by 24 nucleotides
Pf1N1B4_3793 - COG2010: Cytochrome c, mono- and diheme variants, at 4,201,328 to 4,202,149
_3793
Pf1N1B4_3794 - hypothetical protein, at 4,202,148 to 4,202,318
_3794
Pf1N1B4_3795 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 4,202,796 to 4,204,001
_3795
Pf1N1B4_3796 - Enoyl-CoA hydratase [isoleucine degradation] (EC 4.2.1.17) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3), at 4,204,026 to 4,206,170
_3796
Position (kb)
4202
4203
4204
4205 Strain fitness (log2 ratio)
-2
-1
0
1 at 4201.843 kb on + strand, within Pf1N1B4_3793 at 4201.956 kb on + strand, within Pf1N1B4_3793 at 4201.957 kb on - strand, within Pf1N1B4_3793 at 4202.216 kb on - strand, within Pf1N1B4_3794 at 4202.216 kb on - strand, within Pf1N1B4_3794 at 4202.290 kb on + strand, within Pf1N1B4_3794 at 4202.300 kb on + strand, within Pf1N1B4_3794 at 4202.300 kb on + strand, within Pf1N1B4_3794 at 4202.301 kb on - strand at 4202.301 kb on - strand, within Pf1N1B4_3794 at 4202.301 kb on - strand, within Pf1N1B4_3794 at 4202.416 kb on - strand at 4202.457 kb on - strand at 4202.464 kb on + strand at 4202.465 kb on - strand at 4202.465 kb on - strand at 4202.465 kb on - strand at 4202.492 kb on + strand at 4202.492 kb on + strand at 4202.514 kb on - strand at 4202.547 kb on - strand at 4202.576 kb on - strand at 4202.687 kb on + strand at 4202.687 kb on + strand at 4202.687 kb on + strand at 4202.687 kb on + strand at 4202.700 kb on + strand at 4202.700 kb on + strand at 4202.701 kb on - strand at 4202.812 kb on + strand at 4203.513 kb on + strand, within Pf1N1B4_3795 at 4203.545 kb on + strand at 4203.545 kb on + strand, within Pf1N1B4_3795 at 4203.546 kb on - strand, within Pf1N1B4_3795 at 4204.123 kb on - strand at 4204.123 kb on - strand at 4204.276 kb on - strand, within Pf1N1B4_3796 at 4204.525 kb on - strand, within Pf1N1B4_3796 at 4204.731 kb on - strand, within Pf1N1B4_3796 at 4204.965 kb on + strand, within Pf1N1B4_3796
Per-strain Table
Position Strand Gene LocusTag Fraction LB 30C remove 4,201,843 + Pf1N1B4_3793 0.63 +0.9 4,201,956 + Pf1N1B4_3793 0.76 -0.6 4,201,957 - Pf1N1B4_3793 0.77 +0.9 4,202,216 - Pf1N1B4_3794 0.40 -0.5 4,202,216 - Pf1N1B4_3794 0.40 -0.1 4,202,290 + Pf1N1B4_3794 0.83 +1.2 4,202,300 + Pf1N1B4_3794 0.89 +0.5 4,202,300 + Pf1N1B4_3794 0.89 -0.2 4,202,301 - +1.6 4,202,301 - Pf1N1B4_3794 0.89 +0.5 4,202,301 - Pf1N1B4_3794 0.89 +1.3 4,202,416 - +0.3 4,202,457 - +0.0 4,202,464 + +0.9 4,202,465 - +0.8 4,202,465 - -0.2 4,202,465 - +0.7 4,202,492 + +0.5 4,202,492 + -0.6 4,202,514 - -0.8 4,202,547 - -1.3 4,202,576 - -1.5 4,202,687 + -1.4 4,202,687 + -0.4 4,202,687 + +0.4 4,202,687 + +0.5 4,202,700 + -0.8 4,202,700 + +0.1 4,202,701 - -0.1 4,202,812 + -0.2 4,203,513 + Pf1N1B4_3795 0.59 -1.3 4,203,545 + +0.1 4,203,545 + Pf1N1B4_3795 0.62 -2.1 4,203,546 - Pf1N1B4_3795 0.62 -0.2 4,204,123 - -1.6 4,204,123 - +0.7 4,204,276 - Pf1N1B4_3796 0.12 +0.6 4,204,525 - Pf1N1B4_3796 0.23 +0.0 4,204,731 - Pf1N1B4_3796 0.33 +0.3 4,204,965 + Pf1N1B4_3796 0.44 -1.0
Or see this region's nucleotide sequence