Experiment: LB 30C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_27 and Pf1N1B4_28 are separated by 235 nucleotides Pf1N1B4_28 and Pf1N1B4_29 overlap by 4 nucleotides Pf1N1B4_29 and Pf1N1B4_5164 are separated by 225 nucleotides Pf1N1B4_5164 and Pf1N1B4_30 are separated by 102 nucleotides
Pf1N1B4_27 - Biosynthetic Aromatic amino acid aminotransferase alpha (EC 2.6.1.57) @ Aromatic-amino-acid aminotransferase (EC 2.6.1.57), at 30,553 to 31,749
_27
Pf1N1B4_28 - FIG00958186: hypothetical protein, at 31,985 to 32,185
_28
Pf1N1B4_29 - FIG00967200: hypothetical protein, at 32,182 to 32,412
_29
Pf1N1B4_5164 - tRNA-Asn-GTT, at 32,638 to 32,710
_5164
Pf1N1B4_30 - Cation efflux system protein, at 32,813 to 35,899
_30
Position (kb)
31
32
33 Strain fitness (log2 ratio)
-2
-1
0
1 at 31.006 kb on + strand, within Pf1N1B4_27 at 31.007 kb on - strand at 31.405 kb on + strand, within Pf1N1B4_27 at 31.405 kb on + strand, within Pf1N1B4_27 at 31.405 kb on + strand, within Pf1N1B4_27 at 31.405 kb on + strand, within Pf1N1B4_27 at 31.406 kb on - strand, within Pf1N1B4_27 at 31.606 kb on + strand, within Pf1N1B4_27 at 31.631 kb on + strand at 31.631 kb on + strand at 31.660 kb on + strand at 31.660 kb on + strand at 31.660 kb on + strand at 31.660 kb on + strand at 31.660 kb on + strand at 31.661 kb on - strand at 31.661 kb on - strand at 31.661 kb on - strand at 31.661 kb on - strand at 31.762 kb on + strand at 31.781 kb on - strand at 31.797 kb on + strand at 31.798 kb on - strand at 31.810 kb on + strand at 31.892 kb on - strand at 31.952 kb on + strand at 31.952 kb on + strand at 31.958 kb on + strand at 31.958 kb on + strand at 31.959 kb on - strand at 31.959 kb on - strand at 31.959 kb on - strand at 31.959 kb on - strand at 31.959 kb on - strand at 32.001 kb on + strand at 32.002 kb on - strand at 32.144 kb on + strand, within Pf1N1B4_28 at 32.170 kb on + strand at 32.171 kb on - strand at 32.171 kb on - strand at 32.223 kb on - strand, within Pf1N1B4_29 at 32.223 kb on - strand, within Pf1N1B4_29 at 32.232 kb on - strand, within Pf1N1B4_29 at 32.232 kb on - strand, within Pf1N1B4_29 at 32.232 kb on - strand, within Pf1N1B4_29 at 32.463 kb on + strand at 32.517 kb on + strand at 32.526 kb on - strand at 32.811 kb on + strand at 32.844 kb on + strand at 32.845 kb on - strand at 32.880 kb on + strand at 32.880 kb on + strand at 32.880 kb on + strand at 32.881 kb on - strand at 32.881 kb on - strand at 32.881 kb on - strand at 32.943 kb on + strand at 32.944 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 30C remove 31,006 + Pf1N1B4_27 0.38 +0.1 31,007 - +0.2 31,405 + Pf1N1B4_27 0.71 -0.1 31,405 + Pf1N1B4_27 0.71 +0.4 31,405 + Pf1N1B4_27 0.71 +0.5 31,405 + Pf1N1B4_27 0.71 +0.2 31,406 - Pf1N1B4_27 0.71 -0.1 31,606 + Pf1N1B4_27 0.88 -0.0 31,631 + -0.2 31,631 + +1.6 31,660 + -1.2 31,660 + -1.5 31,660 + +0.4 31,660 + +0.4 31,660 + -0.2 31,661 - -0.9 31,661 - +1.0 31,661 - -0.1 31,661 - -1.1 31,762 + +0.6 31,781 - +0.2 31,797 + -0.5 31,798 - +0.1 31,810 + +1.0 31,892 - -1.0 31,952 + +0.1 31,952 + +0.3 31,958 + +0.8 31,958 + +0.7 31,959 - +0.8 31,959 - +0.7 31,959 - -0.2 31,959 - -0.2 31,959 - +0.3 32,001 + +0.1 32,002 - -0.7 32,144 + Pf1N1B4_28 0.79 -0.1 32,170 + -0.0 32,171 - -1.0 32,171 - +0.9 32,223 - Pf1N1B4_29 0.18 -0.2 32,223 - Pf1N1B4_29 0.18 -0.7 32,232 - Pf1N1B4_29 0.22 -0.1 32,232 - Pf1N1B4_29 0.22 -1.5 32,232 - Pf1N1B4_29 0.22 -1.0 32,463 + +0.4 32,517 + -0.9 32,526 - +0.8 32,811 + -0.7 32,844 + -1.0 32,845 - -0.1 32,880 + +0.3 32,880 + -1.5 32,880 + +0.3 32,881 - -1.9 32,881 - +0.1 32,881 - +0.1 32,943 + -0.9 32,944 - -0.7
Or see this region's nucleotide sequence