Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2233

Experiment: LB 30C

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2232 and Pf1N1B4_2233 are separated by 210 nucleotidesPf1N1B4_2233 and Pf1N1B4_2234 are separated by 404 nucleotides Pf1N1B4_2232 - Ferric iron ABC transporter, iron-binding protein, at 2,544,096 to 2,545,100 _2232 Pf1N1B4_2233 - Ferric iron ABC transporter, permease protein, at 2,545,311 to 2,546,894 _2233 Pf1N1B4_2234 - Aminomethyltransferase (glycine cleavage system T protein) (EC 2.1.2.10), at 2,547,299 to 2,548,132 _2234 Position (kb) 2545 2546 2547Strain fitness (log2 ratio) -2 -1 0 1 2at 2544.429 kb on + strand, within Pf1N1B4_2232at 2544.486 kb on + strand, within Pf1N1B4_2232at 2544.487 kb on - strand, within Pf1N1B4_2232at 2544.525 kb on + strand, within Pf1N1B4_2232at 2544.525 kb on + strand, within Pf1N1B4_2232at 2544.526 kb on - strand, within Pf1N1B4_2232at 2544.526 kb on - strand, within Pf1N1B4_2232at 2544.588 kb on + strand, within Pf1N1B4_2232at 2544.756 kb on + strand, within Pf1N1B4_2232at 2544.756 kb on + strand, within Pf1N1B4_2232at 2544.756 kb on + strand, within Pf1N1B4_2232at 2544.756 kb on + strand, within Pf1N1B4_2232at 2544.756 kb on + strand, within Pf1N1B4_2232at 2544.757 kb on - strand, within Pf1N1B4_2232at 2544.757 kb on - strand, within Pf1N1B4_2232at 2544.757 kb on - strand, within Pf1N1B4_2232at 2544.757 kb on - strand, within Pf1N1B4_2232at 2544.757 kb on - strand, within Pf1N1B4_2232at 2544.759 kb on + strandat 2544.997 kb on + strand, within Pf1N1B4_2232at 2545.031 kb on - strandat 2545.112 kb on + strandat 2545.112 kb on + strandat 2545.112 kb on + strandat 2545.112 kb on + strandat 2545.112 kb on + strandat 2545.112 kb on + strandat 2545.112 kb on + strandat 2545.112 kb on + strandat 2545.112 kb on + strandat 2545.113 kb on - strandat 2545.113 kb on - strandat 2545.113 kb on - strandat 2545.113 kb on - strandat 2545.113 kb on - strandat 2545.113 kb on - strandat 2545.113 kb on - strandat 2545.262 kb on + strandat 2545.575 kb on + strand, within Pf1N1B4_2233at 2545.575 kb on + strand, within Pf1N1B4_2233at 2545.575 kb on + strand, within Pf1N1B4_2233at 2545.575 kb on + strandat 2545.575 kb on + strandat 2545.576 kb on - strand, within Pf1N1B4_2233at 2545.576 kb on - strand, within Pf1N1B4_2233at 2545.576 kb on - strand, within Pf1N1B4_2233at 2545.576 kb on - strand, within Pf1N1B4_2233at 2545.576 kb on - strand, within Pf1N1B4_2233at 2545.576 kb on - strand, within Pf1N1B4_2233at 2545.576 kb on - strand, within Pf1N1B4_2233at 2545.576 kb on - strand, within Pf1N1B4_2233at 2545.576 kb on - strand, within Pf1N1B4_2233at 2545.731 kb on + strand, within Pf1N1B4_2233at 2545.732 kb on - strand, within Pf1N1B4_2233at 2545.732 kb on - strand, within Pf1N1B4_2233at 2545.732 kb on - strand, within Pf1N1B4_2233at 2545.928 kb on + strand, within Pf1N1B4_2233at 2545.947 kb on + strandat 2545.948 kb on - strand, within Pf1N1B4_2233at 2546.032 kb on - strand, within Pf1N1B4_2233at 2546.032 kb on - strand, within Pf1N1B4_2233at 2546.097 kb on + strand, within Pf1N1B4_2233at 2546.097 kb on + strand, within Pf1N1B4_2233at 2546.149 kb on + strand, within Pf1N1B4_2233at 2546.149 kb on + strand, within Pf1N1B4_2233at 2546.150 kb on - strand, within Pf1N1B4_2233at 2546.150 kb on - strand, within Pf1N1B4_2233at 2546.232 kb on + strand, within Pf1N1B4_2233at 2546.232 kb on + strand, within Pf1N1B4_2233at 2546.259 kb on + strand, within Pf1N1B4_2233at 2546.260 kb on - strand, within Pf1N1B4_2233at 2546.427 kb on + strand, within Pf1N1B4_2233at 2546.428 kb on - strandat 2546.523 kb on + strand, within Pf1N1B4_2233at 2546.524 kb on - strand, within Pf1N1B4_2233at 2546.609 kb on + strand, within Pf1N1B4_2233at 2546.610 kb on - strand, within Pf1N1B4_2233at 2546.649 kb on + strand, within Pf1N1B4_2233at 2546.649 kb on + strand, within Pf1N1B4_2233at 2546.649 kb on + strand, within Pf1N1B4_2233at 2546.650 kb on - strand, within Pf1N1B4_2233at 2546.650 kb on - strand, within Pf1N1B4_2233at 2546.892 kb on + strandat 2546.892 kb on + strandat 2546.905 kb on + strandat 2546.906 kb on - strandat 2546.906 kb on - strandat 2546.918 kb on + strandat 2546.918 kb on + strandat 2546.918 kb on + strandat 2546.919 kb on - strandat 2547.063 kb on - strandat 2547.073 kb on - strandat 2547.073 kb on - strandat 2547.196 kb on + strandat 2547.252 kb on - strandat 2547.255 kb on + strandat 2547.256 kb on - strandat 2547.303 kb on - strandat 2547.303 kb on - strandat 2547.354 kb on - strandat 2547.563 kb on + strand, within Pf1N1B4_2234at 2547.564 kb on - strand, within Pf1N1B4_2234at 2547.691 kb on + strand, within Pf1N1B4_2234at 2547.692 kb on - strand, within Pf1N1B4_2234at 2547.879 kb on + strand, within Pf1N1B4_2234at 2547.879 kb on + strand, within Pf1N1B4_2234

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Per-strain Table

Position Strand Gene LocusTag Fraction LB 30C
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2,544,429 + Pf1N1B4_2232 0.33 +1.0
2,544,486 + Pf1N1B4_2232 0.39 -1.4
2,544,487 - Pf1N1B4_2232 0.39 -1.1
2,544,525 + Pf1N1B4_2232 0.43 -0.0
2,544,525 + Pf1N1B4_2232 0.43 -1.8
2,544,526 - Pf1N1B4_2232 0.43 +0.7
2,544,526 - Pf1N1B4_2232 0.43 -0.1
2,544,588 + Pf1N1B4_2232 0.49 +0.9
2,544,756 + Pf1N1B4_2232 0.66 -1.2
2,544,756 + Pf1N1B4_2232 0.66 -0.5
2,544,756 + Pf1N1B4_2232 0.66 +0.7
2,544,756 + Pf1N1B4_2232 0.66 +0.0
2,544,756 + Pf1N1B4_2232 0.66 -0.1
2,544,757 - Pf1N1B4_2232 0.66 -0.7
2,544,757 - Pf1N1B4_2232 0.66 +0.6
2,544,757 - Pf1N1B4_2232 0.66 -1.1
2,544,757 - Pf1N1B4_2232 0.66 +0.1
2,544,757 - Pf1N1B4_2232 0.66 -0.1
2,544,759 + +0.1
2,544,997 + Pf1N1B4_2232 0.90 -1.4
2,545,031 - +0.0
2,545,112 + +0.2
2,545,112 + +0.7
2,545,112 + +0.0
2,545,112 + -0.6
2,545,112 + -0.3
2,545,112 + +0.1
2,545,112 + +0.9
2,545,112 + -1.7
2,545,112 + -0.4
2,545,113 - +0.3
2,545,113 - +0.1
2,545,113 - +0.9
2,545,113 - +0.4
2,545,113 - -1.1
2,545,113 - -1.8
2,545,113 - -1.0
2,545,262 + +0.9
2,545,575 + Pf1N1B4_2233 0.17 -0.9
2,545,575 + Pf1N1B4_2233 0.17 +0.1
2,545,575 + Pf1N1B4_2233 0.17 +0.7
2,545,575 + -0.6
2,545,575 + +2.4
2,545,576 - Pf1N1B4_2233 0.17 -1.4
2,545,576 - Pf1N1B4_2233 0.17 +0.3
2,545,576 - Pf1N1B4_2233 0.17 -0.8
2,545,576 - Pf1N1B4_2233 0.17 -0.7
2,545,576 - Pf1N1B4_2233 0.17 -1.0
2,545,576 - Pf1N1B4_2233 0.17 +0.1
2,545,576 - Pf1N1B4_2233 0.17 +0.5
2,545,576 - Pf1N1B4_2233 0.17 -1.1
2,545,576 - Pf1N1B4_2233 0.17 -0.3
2,545,731 + Pf1N1B4_2233 0.27 -0.9
2,545,732 - Pf1N1B4_2233 0.27 -1.4
2,545,732 - Pf1N1B4_2233 0.27 -0.7
2,545,732 - Pf1N1B4_2233 0.27 -0.9
2,545,928 + Pf1N1B4_2233 0.39 -1.1
2,545,947 + +0.9
2,545,948 - Pf1N1B4_2233 0.40 -1.4
2,546,032 - Pf1N1B4_2233 0.46 -0.9
2,546,032 - Pf1N1B4_2233 0.46 -1.5
2,546,097 + Pf1N1B4_2233 0.50 -1.2
2,546,097 + Pf1N1B4_2233 0.50 -1.2
2,546,149 + Pf1N1B4_2233 0.53 +0.1
2,546,149 + Pf1N1B4_2233 0.53 -0.7
2,546,150 - Pf1N1B4_2233 0.53 -1.0
2,546,150 - Pf1N1B4_2233 0.53 -0.0
2,546,232 + Pf1N1B4_2233 0.58 -0.1
2,546,232 + Pf1N1B4_2233 0.58 -0.5
2,546,259 + Pf1N1B4_2233 0.60 +0.3
2,546,260 - Pf1N1B4_2233 0.60 -0.3
2,546,427 + Pf1N1B4_2233 0.70 -1.0
2,546,428 - -0.6
2,546,523 + Pf1N1B4_2233 0.77 -0.5
2,546,524 - Pf1N1B4_2233 0.77 -1.9
2,546,609 + Pf1N1B4_2233 0.82 +0.1
2,546,610 - Pf1N1B4_2233 0.82 -0.2
2,546,649 + Pf1N1B4_2233 0.84 -0.0
2,546,649 + Pf1N1B4_2233 0.84 -1.4
2,546,649 + Pf1N1B4_2233 0.84 -0.8
2,546,650 - Pf1N1B4_2233 0.85 -0.3
2,546,650 - Pf1N1B4_2233 0.85 -0.1
2,546,892 + +0.1
2,546,892 + -0.2
2,546,905 + +1.3
2,546,906 - +0.6
2,546,906 - -1.7
2,546,918 + -0.4
2,546,918 + -0.3
2,546,918 + +0.3
2,546,919 - +0.3
2,547,063 - +0.1
2,547,073 - +0.4
2,547,073 - +0.3
2,547,196 + +0.2
2,547,252 - +0.4
2,547,255 + -0.8
2,547,256 - +0.7
2,547,303 - -0.0
2,547,303 - +0.1
2,547,354 - -1.6
2,547,563 + Pf1N1B4_2234 0.32 -1.0
2,547,564 - Pf1N1B4_2234 0.32 -1.1
2,547,691 + Pf1N1B4_2234 0.47 +0.2
2,547,692 - Pf1N1B4_2234 0.47 -2.1
2,547,879 + Pf1N1B4_2234 0.70 -0.3
2,547,879 + Pf1N1B4_2234 0.70 +0.4

Or see this region's nucleotide sequence