Experiment: LB 30C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2232 and Pf1N1B4_2233 are separated by 210 nucleotides Pf1N1B4_2233 and Pf1N1B4_2234 are separated by 404 nucleotides
Pf1N1B4_2232 - Ferric iron ABC transporter, iron-binding protein, at 2,544,096 to 2,545,100
_2232
Pf1N1B4_2233 - Ferric iron ABC transporter, permease protein, at 2,545,311 to 2,546,894
_2233
Pf1N1B4_2234 - Aminomethyltransferase (glycine cleavage system T protein) (EC 2.1.2.10), at 2,547,299 to 2,548,132
_2234
Position (kb)
2545
2546
2547 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2544.429 kb on + strand, within Pf1N1B4_2232 at 2544.486 kb on + strand, within Pf1N1B4_2232 at 2544.487 kb on - strand, within Pf1N1B4_2232 at 2544.525 kb on + strand, within Pf1N1B4_2232 at 2544.525 kb on + strand, within Pf1N1B4_2232 at 2544.526 kb on - strand, within Pf1N1B4_2232 at 2544.526 kb on - strand, within Pf1N1B4_2232 at 2544.588 kb on + strand, within Pf1N1B4_2232 at 2544.756 kb on + strand, within Pf1N1B4_2232 at 2544.756 kb on + strand, within Pf1N1B4_2232 at 2544.756 kb on + strand, within Pf1N1B4_2232 at 2544.756 kb on + strand, within Pf1N1B4_2232 at 2544.756 kb on + strand, within Pf1N1B4_2232 at 2544.757 kb on - strand, within Pf1N1B4_2232 at 2544.757 kb on - strand, within Pf1N1B4_2232 at 2544.757 kb on - strand, within Pf1N1B4_2232 at 2544.757 kb on - strand, within Pf1N1B4_2232 at 2544.757 kb on - strand, within Pf1N1B4_2232 at 2544.759 kb on + strand at 2544.997 kb on + strand, within Pf1N1B4_2232 at 2545.031 kb on - strand at 2545.112 kb on + strand at 2545.112 kb on + strand at 2545.112 kb on + strand at 2545.112 kb on + strand at 2545.112 kb on + strand at 2545.112 kb on + strand at 2545.112 kb on + strand at 2545.112 kb on + strand at 2545.112 kb on + strand at 2545.113 kb on - strand at 2545.113 kb on - strand at 2545.113 kb on - strand at 2545.113 kb on - strand at 2545.113 kb on - strand at 2545.113 kb on - strand at 2545.113 kb on - strand at 2545.262 kb on + strand at 2545.575 kb on + strand, within Pf1N1B4_2233 at 2545.575 kb on + strand, within Pf1N1B4_2233 at 2545.575 kb on + strand, within Pf1N1B4_2233 at 2545.575 kb on + strand at 2545.575 kb on + strand at 2545.576 kb on - strand, within Pf1N1B4_2233 at 2545.576 kb on - strand, within Pf1N1B4_2233 at 2545.576 kb on - strand, within Pf1N1B4_2233 at 2545.576 kb on - strand, within Pf1N1B4_2233 at 2545.576 kb on - strand, within Pf1N1B4_2233 at 2545.576 kb on - strand, within Pf1N1B4_2233 at 2545.576 kb on - strand, within Pf1N1B4_2233 at 2545.576 kb on - strand, within Pf1N1B4_2233 at 2545.576 kb on - strand, within Pf1N1B4_2233 at 2545.731 kb on + strand, within Pf1N1B4_2233 at 2545.732 kb on - strand, within Pf1N1B4_2233 at 2545.732 kb on - strand, within Pf1N1B4_2233 at 2545.732 kb on - strand, within Pf1N1B4_2233 at 2545.928 kb on + strand, within Pf1N1B4_2233 at 2545.947 kb on + strand at 2545.948 kb on - strand, within Pf1N1B4_2233 at 2546.032 kb on - strand, within Pf1N1B4_2233 at 2546.032 kb on - strand, within Pf1N1B4_2233 at 2546.097 kb on + strand, within Pf1N1B4_2233 at 2546.097 kb on + strand, within Pf1N1B4_2233 at 2546.149 kb on + strand, within Pf1N1B4_2233 at 2546.149 kb on + strand, within Pf1N1B4_2233 at 2546.150 kb on - strand, within Pf1N1B4_2233 at 2546.150 kb on - strand, within Pf1N1B4_2233 at 2546.232 kb on + strand, within Pf1N1B4_2233 at 2546.232 kb on + strand, within Pf1N1B4_2233 at 2546.259 kb on + strand, within Pf1N1B4_2233 at 2546.260 kb on - strand, within Pf1N1B4_2233 at 2546.427 kb on + strand, within Pf1N1B4_2233 at 2546.428 kb on - strand at 2546.523 kb on + strand, within Pf1N1B4_2233 at 2546.524 kb on - strand, within Pf1N1B4_2233 at 2546.609 kb on + strand, within Pf1N1B4_2233 at 2546.610 kb on - strand, within Pf1N1B4_2233 at 2546.649 kb on + strand, within Pf1N1B4_2233 at 2546.649 kb on + strand, within Pf1N1B4_2233 at 2546.649 kb on + strand, within Pf1N1B4_2233 at 2546.650 kb on - strand, within Pf1N1B4_2233 at 2546.650 kb on - strand, within Pf1N1B4_2233 at 2546.892 kb on + strand at 2546.892 kb on + strand at 2546.905 kb on + strand at 2546.906 kb on - strand at 2546.906 kb on - strand at 2546.918 kb on + strand at 2546.918 kb on + strand at 2546.918 kb on + strand at 2546.919 kb on - strand at 2547.063 kb on - strand at 2547.073 kb on - strand at 2547.073 kb on - strand at 2547.196 kb on + strand at 2547.252 kb on - strand at 2547.255 kb on + strand at 2547.256 kb on - strand at 2547.303 kb on - strand at 2547.303 kb on - strand at 2547.354 kb on - strand at 2547.563 kb on + strand, within Pf1N1B4_2234 at 2547.564 kb on - strand, within Pf1N1B4_2234 at 2547.691 kb on + strand, within Pf1N1B4_2234 at 2547.692 kb on - strand, within Pf1N1B4_2234 at 2547.879 kb on + strand, within Pf1N1B4_2234 at 2547.879 kb on + strand, within Pf1N1B4_2234
Per-strain Table
Position Strand Gene LocusTag Fraction LB 30C remove 2,544,429 + Pf1N1B4_2232 0.33 +1.0 2,544,486 + Pf1N1B4_2232 0.39 -1.4 2,544,487 - Pf1N1B4_2232 0.39 -1.1 2,544,525 + Pf1N1B4_2232 0.43 -0.0 2,544,525 + Pf1N1B4_2232 0.43 -1.8 2,544,526 - Pf1N1B4_2232 0.43 +0.7 2,544,526 - Pf1N1B4_2232 0.43 -0.1 2,544,588 + Pf1N1B4_2232 0.49 +0.9 2,544,756 + Pf1N1B4_2232 0.66 -1.2 2,544,756 + Pf1N1B4_2232 0.66 -0.5 2,544,756 + Pf1N1B4_2232 0.66 +0.7 2,544,756 + Pf1N1B4_2232 0.66 +0.0 2,544,756 + Pf1N1B4_2232 0.66 -0.1 2,544,757 - Pf1N1B4_2232 0.66 -0.7 2,544,757 - Pf1N1B4_2232 0.66 +0.6 2,544,757 - Pf1N1B4_2232 0.66 -1.1 2,544,757 - Pf1N1B4_2232 0.66 +0.1 2,544,757 - Pf1N1B4_2232 0.66 -0.1 2,544,759 + +0.1 2,544,997 + Pf1N1B4_2232 0.90 -1.4 2,545,031 - +0.0 2,545,112 + +0.2 2,545,112 + +0.7 2,545,112 + +0.0 2,545,112 + -0.6 2,545,112 + -0.3 2,545,112 + +0.1 2,545,112 + +0.9 2,545,112 + -1.7 2,545,112 + -0.4 2,545,113 - +0.3 2,545,113 - +0.1 2,545,113 - +0.9 2,545,113 - +0.4 2,545,113 - -1.1 2,545,113 - -1.8 2,545,113 - -1.0 2,545,262 + +0.9 2,545,575 + Pf1N1B4_2233 0.17 -0.9 2,545,575 + Pf1N1B4_2233 0.17 +0.1 2,545,575 + Pf1N1B4_2233 0.17 +0.7 2,545,575 + -0.6 2,545,575 + +2.4 2,545,576 - Pf1N1B4_2233 0.17 -1.4 2,545,576 - Pf1N1B4_2233 0.17 +0.3 2,545,576 - Pf1N1B4_2233 0.17 -0.8 2,545,576 - Pf1N1B4_2233 0.17 -0.7 2,545,576 - Pf1N1B4_2233 0.17 -1.0 2,545,576 - Pf1N1B4_2233 0.17 +0.1 2,545,576 - Pf1N1B4_2233 0.17 +0.5 2,545,576 - Pf1N1B4_2233 0.17 -1.1 2,545,576 - Pf1N1B4_2233 0.17 -0.3 2,545,731 + Pf1N1B4_2233 0.27 -0.9 2,545,732 - Pf1N1B4_2233 0.27 -1.4 2,545,732 - Pf1N1B4_2233 0.27 -0.7 2,545,732 - Pf1N1B4_2233 0.27 -0.9 2,545,928 + Pf1N1B4_2233 0.39 -1.1 2,545,947 + +0.9 2,545,948 - Pf1N1B4_2233 0.40 -1.4 2,546,032 - Pf1N1B4_2233 0.46 -0.9 2,546,032 - Pf1N1B4_2233 0.46 -1.5 2,546,097 + Pf1N1B4_2233 0.50 -1.2 2,546,097 + Pf1N1B4_2233 0.50 -1.2 2,546,149 + Pf1N1B4_2233 0.53 +0.1 2,546,149 + Pf1N1B4_2233 0.53 -0.7 2,546,150 - Pf1N1B4_2233 0.53 -1.0 2,546,150 - Pf1N1B4_2233 0.53 -0.0 2,546,232 + Pf1N1B4_2233 0.58 -0.1 2,546,232 + Pf1N1B4_2233 0.58 -0.5 2,546,259 + Pf1N1B4_2233 0.60 +0.3 2,546,260 - Pf1N1B4_2233 0.60 -0.3 2,546,427 + Pf1N1B4_2233 0.70 -1.0 2,546,428 - -0.6 2,546,523 + Pf1N1B4_2233 0.77 -0.5 2,546,524 - Pf1N1B4_2233 0.77 -1.9 2,546,609 + Pf1N1B4_2233 0.82 +0.1 2,546,610 - Pf1N1B4_2233 0.82 -0.2 2,546,649 + Pf1N1B4_2233 0.84 -0.0 2,546,649 + Pf1N1B4_2233 0.84 -1.4 2,546,649 + Pf1N1B4_2233 0.84 -0.8 2,546,650 - Pf1N1B4_2233 0.85 -0.3 2,546,650 - Pf1N1B4_2233 0.85 -0.1 2,546,892 + +0.1 2,546,892 + -0.2 2,546,905 + +1.3 2,546,906 - +0.6 2,546,906 - -1.7 2,546,918 + -0.4 2,546,918 + -0.3 2,546,918 + +0.3 2,546,919 - +0.3 2,547,063 - +0.1 2,547,073 - +0.4 2,547,073 - +0.3 2,547,196 + +0.2 2,547,252 - +0.4 2,547,255 + -0.8 2,547,256 - +0.7 2,547,303 - -0.0 2,547,303 - +0.1 2,547,354 - -1.6 2,547,563 + Pf1N1B4_2234 0.32 -1.0 2,547,564 - Pf1N1B4_2234 0.32 -1.1 2,547,691 + Pf1N1B4_2234 0.47 +0.2 2,547,692 - Pf1N1B4_2234 0.47 -2.1 2,547,879 + Pf1N1B4_2234 0.70 -0.3 2,547,879 + Pf1N1B4_2234 0.70 +0.4
Or see this region's nucleotide sequence