Experiment: D-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25975 and BWI76_RS25980 are separated by 46 nucleotides BWI76_RS25980 and BWI76_RS25985 overlap by 41 nucleotides BWI76_RS25985 and BWI76_RS25990 are separated by 157 nucleotides BWI76_RS25990 and BWI76_RS25995 are separated by 51 nucleotides
BWI76_RS25975: BWI76_RS25975 - phosphoribulokinase, at 5,369,470 to 5,370,339
_RS25975
BWI76_RS25980: BWI76_RS25980 - hypothetical protein, at 5,370,386 to 5,370,790
_RS25980
BWI76_RS25985: BWI76_RS25985 - hypothetical protein, at 5,370,750 to 5,370,937
_RS25985
BWI76_RS25990: BWI76_RS25990 - cAMP-activated global transcriptional regulator CRP, at 5,371,095 to 5,371,727
_RS25990
BWI76_RS25995: BWI76_RS25995 - membrane protein, at 5,371,779 to 5,373,857
_RS25995
Position (kb)
5371
5372 Strain fitness (log2 ratio)
-2
-1
0
1 at 5370.107 kb on + strand, within BWI76_RS25975 at 5370.115 kb on - strand, within BWI76_RS25975 at 5370.493 kb on - strand, within BWI76_RS25980 at 5370.493 kb on - strand, within BWI76_RS25980 at 5370.569 kb on + strand, within BWI76_RS25980 at 5370.968 kb on + strand at 5370.971 kb on - strand at 5370.992 kb on + strand at 5371.814 kb on - strand at 5371.890 kb on + strand at 5372.219 kb on + strand, within BWI76_RS25995 at 5372.276 kb on - strand, within BWI76_RS25995 at 5372.310 kb on + strand, within BWI76_RS25995 at 5372.400 kb on + strand, within BWI76_RS25995 at 5372.530 kb on - strand, within BWI76_RS25995 at 5372.598 kb on - strand, within BWI76_RS25995
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (C) remove 5,370,107 + BWI76_RS25975 0.73 -1.1 5,370,115 - BWI76_RS25975 0.74 -0.4 5,370,493 - BWI76_RS25980 0.26 -0.9 5,370,493 - BWI76_RS25980 0.26 -0.2 5,370,569 + BWI76_RS25980 0.45 -0.1 5,370,968 + -2.0 5,370,971 - -2.5 5,370,992 + -1.7 5,371,814 - -0.1 5,371,890 + -0.1 5,372,219 + BWI76_RS25995 0.21 -0.2 5,372,276 - BWI76_RS25995 0.24 +0.5 5,372,310 + BWI76_RS25995 0.26 -0.2 5,372,400 + BWI76_RS25995 0.30 +0.6 5,372,530 - BWI76_RS25995 0.36 +1.6 5,372,598 - BWI76_RS25995 0.39 +0.4
Or see this region's nucleotide sequence