Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS03075

Experiment: D-Alanine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS03070 and BWI76_RS03075 are separated by 18 nucleotidesBWI76_RS03075 and BWI76_RS03080 are separated by 196 nucleotides BWI76_RS03070: BWI76_RS03070 - Methylmalonate-semialdehyde dehydrogenase [inositol] (EC 1.2.1.27) (from data), at 629,200 to 630,705 _RS03070 BWI76_RS03075: BWI76_RS03075 - 5-deoxy-D-glucuronate isomerase (EC 5.3.1.30) (from data), at 630,724 to 631,533 _RS03075 BWI76_RS03080: BWI76_RS03080 - (Fe-S)-cluster assembly protein, at 631,730 to 632,587 _RS03080 Position (kb) 630 631 632Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 629.832 kb on + strand, within BWI76_RS03070at 629.837 kb on + strand, within BWI76_RS03070at 629.840 kb on - strand, within BWI76_RS03070at 629.845 kb on - strand, within BWI76_RS03070at 629.947 kb on + strand, within BWI76_RS03070at 630.110 kb on - strand, within BWI76_RS03070at 630.152 kb on + strand, within BWI76_RS03070at 630.239 kb on + strand, within BWI76_RS03070at 630.394 kb on + strand, within BWI76_RS03070at 630.516 kb on + strand, within BWI76_RS03070at 630.517 kb on - strand, within BWI76_RS03070at 630.819 kb on - strand, within BWI76_RS03075at 630.869 kb on + strand, within BWI76_RS03075at 630.899 kb on - strand, within BWI76_RS03075at 630.926 kb on + strand, within BWI76_RS03075at 630.990 kb on + strand, within BWI76_RS03075at 630.990 kb on + strand, within BWI76_RS03075at 630.998 kb on - strand, within BWI76_RS03075at 631.193 kb on - strand, within BWI76_RS03075at 631.350 kb on + strand, within BWI76_RS03075at 631.367 kb on + strand, within BWI76_RS03075at 631.471 kb on + strandat 631.508 kb on + strandat 631.639 kb on - strandat 631.711 kb on + strandat 631.852 kb on + strand, within BWI76_RS03080at 631.860 kb on - strand, within BWI76_RS03080at 631.932 kb on + strand, within BWI76_RS03080at 632.081 kb on - strand, within BWI76_RS03080at 632.086 kb on - strand, within BWI76_RS03080at 632.231 kb on - strand, within BWI76_RS03080

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Alanine (C)
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629,832 + BWI76_RS03070 0.42 +1.7
629,837 + BWI76_RS03070 0.42 -0.7
629,840 - BWI76_RS03070 0.42 -0.4
629,845 - BWI76_RS03070 0.43 +1.1
629,947 + BWI76_RS03070 0.50 +0.1
630,110 - BWI76_RS03070 0.60 +2.0
630,152 + BWI76_RS03070 0.63 -1.9
630,239 + BWI76_RS03070 0.69 +0.6
630,394 + BWI76_RS03070 0.79 +0.3
630,516 + BWI76_RS03070 0.87 -0.5
630,517 - BWI76_RS03070 0.87 -0.0
630,819 - BWI76_RS03075 0.12 -0.1
630,869 + BWI76_RS03075 0.18 +0.9
630,899 - BWI76_RS03075 0.22 -0.9
630,926 + BWI76_RS03075 0.25 +2.3
630,990 + BWI76_RS03075 0.33 +0.6
630,990 + BWI76_RS03075 0.33 +0.0
630,998 - BWI76_RS03075 0.34 -0.2
631,193 - BWI76_RS03075 0.58 +0.7
631,350 + BWI76_RS03075 0.77 +2.9
631,367 + BWI76_RS03075 0.79 -0.7
631,471 + +0.6
631,508 + +0.3
631,639 - -0.3
631,711 + +0.3
631,852 + BWI76_RS03080 0.14 -1.7
631,860 - BWI76_RS03080 0.15 -2.1
631,932 + BWI76_RS03080 0.24 -2.5
632,081 - BWI76_RS03080 0.41 -4.5
632,086 - BWI76_RS03080 0.41 -0.9
632,231 - BWI76_RS03080 0.58 -1.6

Or see this region's nucleotide sequence