Experiment: Formate with Bacitracin 15.6 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08470 and MMP_RS08475 overlap by 4 nucleotides MMP_RS08475 and MMP_RS08480 overlap by 10 nucleotides MMP_RS08480 and MMP_RS08485 are separated by 82 nucleotides
MMP_RS08470: MMP_RS08470 - ATP-binding cassette domain-containing protein, at 1,589,977 to 1,591,038
_RS08470
MMP_RS08475: MMP_RS08475 - ABC transporter permease, at 1,591,035 to 1,591,817
_RS08475
MMP_RS08480: MMP_RS08480 - molybdate ABC transporter substrate-binding protein, at 1,591,808 to 1,592,611
_RS08480
MMP_RS08485: MMP_RS08485 - tungstate ABC transporter substrate-binding protein WtpA, at 1,592,694 to 1,593,692
_RS08485
Position (kb)
1591
1592
1593 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1590.812 kb on + strand, within MMP_RS08470 at 1590.841 kb on - strand, within MMP_RS08470 at 1590.842 kb on + strand, within MMP_RS08470 at 1590.842 kb on + strand, within MMP_RS08470 at 1590.862 kb on - strand, within MMP_RS08470 at 1590.947 kb on + strand at 1590.950 kb on - strand at 1590.951 kb on + strand at 1590.970 kb on - strand at 1591.054 kb on - strand at 1591.093 kb on + strand at 1591.150 kb on - strand, within MMP_RS08475 at 1591.177 kb on - strand, within MMP_RS08475 at 1591.178 kb on + strand, within MMP_RS08475 at 1591.183 kb on - strand, within MMP_RS08475 at 1591.286 kb on - strand, within MMP_RS08475 at 1591.374 kb on - strand, within MMP_RS08475 at 1591.374 kb on - strand, within MMP_RS08475 at 1591.374 kb on - strand, within MMP_RS08475 at 1591.374 kb on - strand, within MMP_RS08475 at 1591.374 kb on - strand, within MMP_RS08475 at 1591.375 kb on + strand, within MMP_RS08475 at 1591.515 kb on - strand, within MMP_RS08475 at 1591.728 kb on - strand, within MMP_RS08475 at 1591.729 kb on + strand, within MMP_RS08475 at 1591.729 kb on + strand, within MMP_RS08475 at 1591.733 kb on - strand, within MMP_RS08475 at 1591.755 kb on - strand at 1591.865 kb on - strand at 1591.971 kb on + strand, within MMP_RS08480 at 1592.000 kb on + strand, within MMP_RS08480 at 1592.050 kb on + strand, within MMP_RS08480 at 1592.050 kb on + strand, within MMP_RS08480 at 1592.130 kb on + strand, within MMP_RS08480 at 1592.131 kb on - strand, within MMP_RS08480 at 1592.271 kb on + strand, within MMP_RS08480 at 1592.271 kb on + strand, within MMP_RS08480 at 1592.291 kb on - strand, within MMP_RS08480 at 1592.299 kb on + strand, within MMP_RS08480 at 1592.349 kb on + strand, within MMP_RS08480 at 1592.403 kb on - strand, within MMP_RS08480 at 1592.451 kb on + strand, within MMP_RS08480 at 1592.451 kb on + strand, within MMP_RS08480 at 1592.461 kb on - strand, within MMP_RS08480 at 1592.592 kb on + strand at 1592.636 kb on + strand at 1592.800 kb on + strand, within MMP_RS08485 at 1592.908 kb on + strand, within MMP_RS08485 at 1592.984 kb on + strand, within MMP_RS08485 at 1593.016 kb on - strand, within MMP_RS08485 at 1593.021 kb on + strand, within MMP_RS08485 at 1593.028 kb on - strand, within MMP_RS08485 at 1593.035 kb on + strand, within MMP_RS08485 at 1593.087 kb on - strand, within MMP_RS08485 at 1593.123 kb on - strand, within MMP_RS08485 at 1593.133 kb on - strand, within MMP_RS08485 at 1593.133 kb on - strand, within MMP_RS08485 at 1593.171 kb on + strand, within MMP_RS08485 at 1593.194 kb on + strand, within MMP_RS08485 at 1593.287 kb on + strand, within MMP_RS08485 at 1593.436 kb on + strand, within MMP_RS08485 at 1593.436 kb on + strand, within MMP_RS08485 at 1593.548 kb on + strand, within MMP_RS08485 at 1593.560 kb on + strand, within MMP_RS08485 at 1593.610 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate with Bacitracin 15.6 uM remove 1,590,812 + MMP_RS08470 0.79 -1.3 1,590,841 - MMP_RS08470 0.81 -1.4 1,590,842 + MMP_RS08470 0.81 +0.9 1,590,842 + MMP_RS08470 0.81 -1.4 1,590,862 - MMP_RS08470 0.83 +1.5 1,590,947 + +0.3 1,590,950 - +1.2 1,590,951 + +0.7 1,590,970 - -0.1 1,591,054 - -0.7 1,591,093 + +1.3 1,591,150 - MMP_RS08475 0.15 +1.5 1,591,177 - MMP_RS08475 0.18 +0.7 1,591,178 + MMP_RS08475 0.18 +0.2 1,591,183 - MMP_RS08475 0.19 -0.5 1,591,286 - MMP_RS08475 0.32 -0.8 1,591,374 - MMP_RS08475 0.43 -1.5 1,591,374 - MMP_RS08475 0.43 +1.0 1,591,374 - MMP_RS08475 0.43 -0.3 1,591,374 - MMP_RS08475 0.43 -1.5 1,591,374 - MMP_RS08475 0.43 +0.2 1,591,375 + MMP_RS08475 0.43 -1.4 1,591,515 - MMP_RS08475 0.61 -1.3 1,591,728 - MMP_RS08475 0.89 -0.3 1,591,729 + MMP_RS08475 0.89 +0.2 1,591,729 + MMP_RS08475 0.89 +1.7 1,591,733 - MMP_RS08475 0.89 -2.2 1,591,755 - +0.3 1,591,865 - +1.1 1,591,971 + MMP_RS08480 0.20 -0.4 1,592,000 + MMP_RS08480 0.24 +0.0 1,592,050 + MMP_RS08480 0.30 -0.7 1,592,050 + MMP_RS08480 0.30 -0.8 1,592,130 + MMP_RS08480 0.40 -2.5 1,592,131 - MMP_RS08480 0.40 -0.7 1,592,271 + MMP_RS08480 0.58 -0.0 1,592,271 + MMP_RS08480 0.58 -1.0 1,592,291 - MMP_RS08480 0.60 +0.3 1,592,299 + MMP_RS08480 0.61 +1.3 1,592,349 + MMP_RS08480 0.67 -1.1 1,592,403 - MMP_RS08480 0.74 -0.0 1,592,451 + MMP_RS08480 0.80 -2.3 1,592,451 + MMP_RS08480 0.80 -1.5 1,592,461 - MMP_RS08480 0.81 -0.7 1,592,592 + +0.7 1,592,636 + -2.4 1,592,800 + MMP_RS08485 0.11 +1.6 1,592,908 + MMP_RS08485 0.21 +0.9 1,592,984 + MMP_RS08485 0.29 +2.2 1,593,016 - MMP_RS08485 0.32 +0.7 1,593,021 + MMP_RS08485 0.33 -0.6 1,593,028 - MMP_RS08485 0.33 -2.0 1,593,035 + MMP_RS08485 0.34 -0.9 1,593,087 - MMP_RS08485 0.39 -1.9 1,593,123 - MMP_RS08485 0.43 +0.5 1,593,133 - MMP_RS08485 0.44 +1.2 1,593,133 - MMP_RS08485 0.44 +0.2 1,593,171 + MMP_RS08485 0.48 +0.7 1,593,194 + MMP_RS08485 0.50 +1.0 1,593,287 + MMP_RS08485 0.59 -0.3 1,593,436 + MMP_RS08485 0.74 +0.9 1,593,436 + MMP_RS08485 0.74 +0.4 1,593,548 + MMP_RS08485 0.85 -0.4 1,593,560 + MMP_RS08485 0.87 +1.3 1,593,610 + +0.5
Or see this region's nucleotide sequence