Experiment: Formate with Bacitracin 15.6 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS07115 and MMP_RS07120 are separated by 216 nucleotides MMP_RS07120 and MMP_RS07125 are separated by 102 nucleotides MMP_RS07125 and MMP_RS07130 are separated by 14 nucleotides
MMP_RS07115: MMP_RS07115 - MBL fold metallo-hydrolase, at 1,360,985 to 1,362,253
_RS07115
MMP_RS07120: MMP_RS07120 - coenzyme F420 hydrogenase subunit alpha, at 1,362,470 to 1,363,714
_RS07120
MMP_RS07125: MMP_RS07125 - coenzyme F420-reducing hydrogenase FrhD protein, at 1,363,817 to 1,364,341
_RS07125
MMP_RS07130: MMP_RS07130 - coenzyme F420 hydrogenase subunit gamma, at 1,364,356 to 1,365,042
_RS07130
Position (kb)
1362
1363
1364 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1361.508 kb on - strand, within MMP_RS07115 at 1361.538 kb on - strand, within MMP_RS07115 at 1361.542 kb on - strand, within MMP_RS07115 at 1361.544 kb on - strand, within MMP_RS07115 at 1361.545 kb on + strand, within MMP_RS07115 at 1361.679 kb on + strand, within MMP_RS07115 at 1361.867 kb on + strand, within MMP_RS07115 at 1361.950 kb on - strand, within MMP_RS07115 at 1361.953 kb on + strand, within MMP_RS07115 at 1362.131 kb on + strand at 1362.224 kb on + strand at 1362.264 kb on - strand at 1362.447 kb on - strand at 1362.488 kb on + strand at 1362.570 kb on + strand at 1362.589 kb on + strand at 1362.661 kb on - strand, within MMP_RS07120 at 1362.663 kb on - strand, within MMP_RS07120 at 1362.664 kb on + strand, within MMP_RS07120 at 1362.664 kb on + strand, within MMP_RS07120 at 1362.760 kb on + strand, within MMP_RS07120 at 1362.765 kb on - strand, within MMP_RS07120 at 1362.793 kb on + strand, within MMP_RS07120 at 1362.810 kb on - strand, within MMP_RS07120 at 1362.853 kb on + strand, within MMP_RS07120 at 1362.904 kb on + strand, within MMP_RS07120 at 1363.039 kb on + strand, within MMP_RS07120 at 1363.111 kb on + strand, within MMP_RS07120 at 1363.111 kb on + strand, within MMP_RS07120 at 1363.115 kb on - strand, within MMP_RS07120 at 1363.116 kb on + strand, within MMP_RS07120 at 1363.193 kb on - strand, within MMP_RS07120 at 1363.247 kb on - strand, within MMP_RS07120 at 1363.248 kb on + strand, within MMP_RS07120 at 1363.399 kb on + strand, within MMP_RS07120 at 1363.535 kb on - strand, within MMP_RS07120 at 1363.617 kb on - strand at 1363.638 kb on - strand at 1363.638 kb on - strand at 1363.824 kb on + strand at 1363.899 kb on - strand, within MMP_RS07125 at 1363.971 kb on - strand, within MMP_RS07125 at 1364.098 kb on - strand, within MMP_RS07125 at 1364.176 kb on + strand, within MMP_RS07125 at 1364.308 kb on + strand at 1364.381 kb on - strand at 1364.508 kb on + strand, within MMP_RS07130 at 1364.513 kb on - strand, within MMP_RS07130 at 1364.594 kb on - strand, within MMP_RS07130 at 1364.598 kb on + strand, within MMP_RS07130 at 1364.631 kb on - strand, within MMP_RS07130
Per-strain Table
Position Strand Gene LocusTag Fraction Formate with Bacitracin 15.6 uM remove 1,361,508 - MMP_RS07115 0.41 -2.6 1,361,538 - MMP_RS07115 0.44 -0.6 1,361,542 - MMP_RS07115 0.44 -1.3 1,361,544 - MMP_RS07115 0.44 +0.4 1,361,545 + MMP_RS07115 0.44 -0.5 1,361,679 + MMP_RS07115 0.55 -3.8 1,361,867 + MMP_RS07115 0.70 -2.5 1,361,950 - MMP_RS07115 0.76 +1.7 1,361,953 + MMP_RS07115 0.76 -2.0 1,362,131 + -1.5 1,362,224 + -3.6 1,362,264 - -1.4 1,362,447 - -2.0 1,362,488 + -1.9 1,362,570 + -0.8 1,362,589 + -1.0 1,362,661 - MMP_RS07120 0.15 -1.6 1,362,663 - MMP_RS07120 0.16 -1.7 1,362,664 + MMP_RS07120 0.16 -3.2 1,362,664 + MMP_RS07120 0.16 +0.9 1,362,760 + MMP_RS07120 0.23 -2.3 1,362,765 - MMP_RS07120 0.24 -2.0 1,362,793 + MMP_RS07120 0.26 -1.6 1,362,810 - MMP_RS07120 0.27 -4.3 1,362,853 + MMP_RS07120 0.31 -0.9 1,362,904 + MMP_RS07120 0.35 -0.3 1,363,039 + MMP_RS07120 0.46 +0.5 1,363,111 + MMP_RS07120 0.51 -0.5 1,363,111 + MMP_RS07120 0.51 +0.2 1,363,115 - MMP_RS07120 0.52 -2.4 1,363,116 + MMP_RS07120 0.52 -0.5 1,363,193 - MMP_RS07120 0.58 -1.0 1,363,247 - MMP_RS07120 0.62 -1.8 1,363,248 + MMP_RS07120 0.62 -3.7 1,363,399 + MMP_RS07120 0.75 -0.3 1,363,535 - MMP_RS07120 0.86 -1.4 1,363,617 - -1.5 1,363,638 - -0.3 1,363,638 - -0.4 1,363,824 + +0.6 1,363,899 - MMP_RS07125 0.16 -0.6 1,363,971 - MMP_RS07125 0.29 -1.9 1,364,098 - MMP_RS07125 0.54 -3.5 1,364,176 + MMP_RS07125 0.68 +0.0 1,364,308 + -0.2 1,364,381 - -1.2 1,364,508 + MMP_RS07130 0.22 -1.0 1,364,513 - MMP_RS07130 0.23 -1.5 1,364,594 - MMP_RS07130 0.35 +0.8 1,364,598 + MMP_RS07130 0.35 -0.7 1,364,631 - MMP_RS07130 0.40 -2.1
Or see this region's nucleotide sequence