Experiment: Formate with Bacitracin 15.6 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02305 and MMP_RS02310 are separated by 266 nucleotides MMP_RS02310 and MMP_RS02315 are separated by 0 nucleotides MMP_RS02315 and MMP_RS02320 overlap by 14 nucleotides
MMP_RS02305: MMP_RS02305 - DUF378 domain-containing protein, at 434,515 to 434,763
_RS02305
MMP_RS02310: MMP_RS02310 - RimK-like ATPgrasp N-terminal domain-containing protein, at 435,030 to 435,635
_RS02310
MMP_RS02315: MMP_RS02315 - RimK family alpha-L-glutamate ligase, at 435,636 to 436,586
_RS02315
MMP_RS02320: MMP_RS02320 - glutamate-cysteine ligase family protein, at 436,573 to 437,676
_RS02320
Position (kb) 435
436
437 Strain fitness (log2 ratio) -2
-1
0
1
2 at 434.653 kb on + strand, within MMP_RS02305 at 434.665 kb on - strand, within MMP_RS02305 at 434.679 kb on - strand, within MMP_RS02305 at 434.680 kb on + strand, within MMP_RS02305 at 434.680 kb on + strand, within MMP_RS02305 at 434.728 kb on + strand, within MMP_RS02305 at 434.803 kb on - strand at 434.803 kb on - strand at 435.312 kb on - strand, within MMP_RS02310 at 435.922 kb on + strand, within MMP_RS02315 at 436.008 kb on + strand, within MMP_RS02315 at 436.109 kb on + strand, within MMP_RS02315 at 436.278 kb on - strand, within MMP_RS02315 at 436.326 kb on + strand, within MMP_RS02315 at 436.408 kb on + strand, within MMP_RS02315 at 436.473 kb on - strand, within MMP_RS02315 at 436.491 kb on - strand, within MMP_RS02315 at 436.492 kb on + strand at 436.494 kb on + strand at 436.518 kb on - strand at 436.518 kb on - strand at 436.594 kb on - strand at 436.595 kb on + strand at 436.641 kb on + strand at 436.818 kb on - strand, within MMP_RS02320 at 436.818 kb on - strand, within MMP_RS02320 at 436.822 kb on + strand, within MMP_RS02320 at 436.822 kb on + strand, within MMP_RS02320 at 436.832 kb on - strand, within MMP_RS02320 at 436.925 kb on - strand, within MMP_RS02320 at 436.985 kb on + strand, within MMP_RS02320 at 437.007 kb on - strand, within MMP_RS02320 at 437.042 kb on + strand, within MMP_RS02320 at 437.097 kb on - strand, within MMP_RS02320 at 437.098 kb on + strand, within MMP_RS02320 at 437.483 kb on - strand, within MMP_RS02320 at 437.541 kb on + strand, within MMP_RS02320 at 437.586 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate with Bacitracin 15.6 uM remove 434,653 + MMP_RS02305 0.55 +0.7 434,665 - MMP_RS02305 0.60 -0.7 434,679 - MMP_RS02305 0.66 +0.2 434,680 + MMP_RS02305 0.66 +0.3 434,680 + MMP_RS02305 0.66 -0.6 434,728 + MMP_RS02305 0.86 -0.8 434,803 - +0.1 434,803 - +0.0 435,312 - MMP_RS02310 0.47 +0.9 435,922 + MMP_RS02315 0.30 +0.2 436,008 + MMP_RS02315 0.39 -1.2 436,109 + MMP_RS02315 0.50 -1.7 436,278 - MMP_RS02315 0.68 -1.2 436,326 + MMP_RS02315 0.73 -0.9 436,408 + MMP_RS02315 0.81 -0.5 436,473 - MMP_RS02315 0.88 -0.1 436,491 - MMP_RS02315 0.90 -0.8 436,492 + +0.1 436,494 + +0.3 436,518 - -0.0 436,518 - +0.5 436,594 - +1.8 436,595 + -0.9 436,641 + -0.6 436,818 - MMP_RS02320 0.22 +1.0 436,818 - MMP_RS02320 0.22 -1.1 436,822 + MMP_RS02320 0.23 -0.2 436,822 + MMP_RS02320 0.23 -1.4 436,832 - MMP_RS02320 0.23 -0.2 436,925 - MMP_RS02320 0.32 +0.5 436,985 + MMP_RS02320 0.37 -0.0 437,007 - MMP_RS02320 0.39 -0.9 437,042 + MMP_RS02320 0.42 -2.0 437,097 - MMP_RS02320 0.47 -0.5 437,098 + MMP_RS02320 0.48 -1.3 437,483 - MMP_RS02320 0.82 +0.0 437,541 + MMP_RS02320 0.88 -1.1 437,586 - +0.1
Or see this region's nucleotide sequence