Experiment: Formate with Bacitracin 15.6 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00100 and MMP_RS00105 are separated by 12 nucleotides MMP_RS00105 and MMP_RS00110 are separated by 14 nucleotides MMP_RS00110 and MMP_RS00115 are separated by 120 nucleotides
MMP_RS00100: MMP_RS00100 - tRNA pseudouridine(13) synthase TruD, at 24,061 to 25,230
_RS00100
MMP_RS00105: MMP_RS00105 - hypothetical protein, at 25,243 to 26,046
_RS00105
MMP_RS00110: MMP_RS00110 - hypothetical protein, at 26,061 to 26,552
_RS00110
MMP_RS00115: MMP_RS00115 - radical SAM protein, at 26,673 to 27,725
_RS00115
Position (kb) 25
26
27 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 24.425 kb on - strand, within MMP_RS00100 at 24.425 kb on - strand, within MMP_RS00100 at 24.425 kb on - strand, within MMP_RS00100 at 24.425 kb on - strand, within MMP_RS00100 at 24.426 kb on + strand, within MMP_RS00100 at 24.426 kb on + strand, within MMP_RS00100 at 24.426 kb on + strand, within MMP_RS00100 at 24.426 kb on + strand, within MMP_RS00100 at 24.426 kb on + strand, within MMP_RS00100 at 24.426 kb on + strand, within MMP_RS00100 at 24.427 kb on - strand, within MMP_RS00100 at 24.428 kb on + strand, within MMP_RS00100 at 24.458 kb on + strand, within MMP_RS00100 at 24.488 kb on - strand, within MMP_RS00100 at 24.489 kb on + strand, within MMP_RS00100 at 24.529 kb on + strand, within MMP_RS00100 at 24.532 kb on - strand, within MMP_RS00100 at 24.533 kb on + strand, within MMP_RS00100 at 24.580 kb on - strand, within MMP_RS00100 at 24.581 kb on + strand, within MMP_RS00100 at 24.600 kb on - strand, within MMP_RS00100 at 24.608 kb on + strand, within MMP_RS00100 at 24.626 kb on - strand, within MMP_RS00100 at 24.973 kb on + strand, within MMP_RS00100 at 25.183 kb on - strand at 25.196 kb on + strand at 25.239 kb on - strand at 25.240 kb on + strand at 25.265 kb on + strand at 25.274 kb on + strand at 25.278 kb on - strand at 25.279 kb on + strand at 25.408 kb on + strand, within MMP_RS00105 at 25.519 kb on + strand, within MMP_RS00105 at 25.557 kb on + strand, within MMP_RS00105 at 25.741 kb on + strand, within MMP_RS00105 at 25.784 kb on + strand, within MMP_RS00105 at 25.789 kb on - strand, within MMP_RS00105 at 25.789 kb on - strand, within MMP_RS00105 at 25.789 kb on - strand, within MMP_RS00105 at 25.793 kb on + strand, within MMP_RS00105 at 25.867 kb on + strand, within MMP_RS00105 at 25.899 kb on - strand, within MMP_RS00105 at 25.904 kb on - strand, within MMP_RS00105 at 25.905 kb on + strand, within MMP_RS00105 at 25.905 kb on + strand, within MMP_RS00105 at 26.026 kb on - strand at 26.028 kb on - strand at 26.049 kb on - strand at 26.099 kb on - strand at 26.109 kb on - strand at 26.110 kb on + strand at 26.122 kb on - strand, within MMP_RS00110 at 26.123 kb on + strand, within MMP_RS00110 at 26.355 kb on - strand, within MMP_RS00110 at 26.368 kb on - strand, within MMP_RS00110 at 26.412 kb on - strand, within MMP_RS00110 at 26.413 kb on + strand, within MMP_RS00110 at 26.564 kb on - strand at 26.587 kb on - strand at 26.587 kb on - strand at 26.587 kb on - strand at 26.600 kb on - strand at 26.600 kb on - strand at 26.600 kb on - strand at 26.600 kb on - strand at 26.600 kb on - strand at 26.600 kb on - strand at 26.601 kb on + strand at 26.601 kb on + strand at 26.601 kb on + strand at 26.601 kb on + strand at 26.601 kb on + strand at 26.601 kb on + strand at 26.602 kb on - strand at 26.602 kb on - strand at 26.602 kb on - strand at 26.602 kb on - strand at 26.602 kb on - strand at 26.602 kb on - strand at 26.603 kb on + strand at 26.603 kb on + strand at 26.603 kb on + strand at 26.603 kb on + strand at 26.603 kb on + strand at 26.604 kb on - strand at 26.604 kb on - strand at 26.604 kb on - strand at 26.604 kb on - strand at 26.604 kb on - strand at 26.604 kb on - strand at 26.605 kb on + strand at 26.605 kb on + strand at 26.605 kb on + strand at 26.605 kb on + strand at 26.605 kb on + strand at 26.605 kb on + strand at 26.605 kb on + strand at 26.607 kb on + strand at 26.635 kb on - strand at 26.654 kb on + strand at 26.654 kb on + strand at 26.660 kb on - strand at 26.660 kb on - strand at 26.660 kb on - strand at 26.661 kb on + strand at 26.665 kb on + strand at 26.665 kb on + strand at 26.665 kb on + strand at 26.666 kb on - strand at 26.667 kb on + strand at 26.670 kb on + strand at 26.670 kb on + strand at 26.670 kb on + strand at 26.671 kb on - strand at 26.672 kb on + strand at 26.672 kb on + strand at 26.702 kb on - strand at 26.750 kb on - strand at 26.751 kb on + strand at 26.751 kb on + strand at 26.774 kb on - strand at 26.774 kb on - strand at 26.794 kb on - strand, within MMP_RS00115 at 26.794 kb on - strand, within MMP_RS00115 at 26.829 kb on - strand, within MMP_RS00115 at 26.852 kb on - strand, within MMP_RS00115 at 26.855 kb on + strand, within MMP_RS00115 at 26.856 kb on - strand, within MMP_RS00115 at 26.856 kb on - strand, within MMP_RS00115 at 26.856 kb on - strand, within MMP_RS00115 at 26.856 kb on - strand, within MMP_RS00115 at 26.856 kb on - strand, within MMP_RS00115 at 26.857 kb on + strand, within MMP_RS00115 at 26.857 kb on + strand, within MMP_RS00115 at 26.857 kb on + strand, within MMP_RS00115 at 26.857 kb on + strand, within MMP_RS00115 at 26.915 kb on + strand, within MMP_RS00115 at 26.939 kb on - strand, within MMP_RS00115 at 26.940 kb on + strand, within MMP_RS00115 at 26.944 kb on + strand, within MMP_RS00115 at 26.981 kb on - strand, within MMP_RS00115 at 26.987 kb on - strand, within MMP_RS00115
Per-strain Table
Position Strand Gene LocusTag Fraction Formate with Bacitracin 15.6 uM remove 24,425 - MMP_RS00100 0.31 -0.7 24,425 - MMP_RS00100 0.31 +0.7 24,425 - MMP_RS00100 0.31 -0.5 24,425 - MMP_RS00100 0.31 -1.5 24,426 + MMP_RS00100 0.31 -0.6 24,426 + MMP_RS00100 0.31 -1.3 24,426 + MMP_RS00100 0.31 -0.1 24,426 + MMP_RS00100 0.31 +1.0 24,426 + MMP_RS00100 0.31 +0.9 24,426 + MMP_RS00100 0.31 -0.1 24,427 - MMP_RS00100 0.31 -1.4 24,428 + MMP_RS00100 0.31 +0.2 24,458 + MMP_RS00100 0.34 -1.9 24,488 - MMP_RS00100 0.36 +0.7 24,489 + MMP_RS00100 0.37 -2.6 24,529 + MMP_RS00100 0.40 -0.7 24,532 - MMP_RS00100 0.40 -0.3 24,533 + MMP_RS00100 0.40 +0.3 24,580 - MMP_RS00100 0.44 -1.5 24,581 + MMP_RS00100 0.44 -1.8 24,600 - MMP_RS00100 0.46 +1.2 24,608 + MMP_RS00100 0.47 -0.4 24,626 - MMP_RS00100 0.48 +1.5 24,973 + MMP_RS00100 0.78 +1.1 25,183 - -0.5 25,196 + +0.5 25,239 - -0.2 25,240 + +0.5 25,265 + -0.4 25,274 + -0.6 25,278 - -0.9 25,279 + +0.1 25,408 + MMP_RS00105 0.21 -2.2 25,519 + MMP_RS00105 0.34 -0.8 25,557 + MMP_RS00105 0.39 +0.1 25,741 + MMP_RS00105 0.62 -1.0 25,784 + MMP_RS00105 0.67 -1.6 25,789 - MMP_RS00105 0.68 -0.5 25,789 - MMP_RS00105 0.68 -3.0 25,789 - MMP_RS00105 0.68 +0.3 25,793 + MMP_RS00105 0.68 +0.2 25,867 + MMP_RS00105 0.78 -2.3 25,899 - MMP_RS00105 0.82 -0.7 25,904 - MMP_RS00105 0.82 -0.3 25,905 + MMP_RS00105 0.82 -1.3 25,905 + MMP_RS00105 0.82 +0.2 26,026 - -0.1 26,028 - -0.6 26,049 - +0.7 26,099 - +1.8 26,109 - +1.8 26,110 + -0.2 26,122 - MMP_RS00110 0.12 +1.0 26,123 + MMP_RS00110 0.13 +0.9 26,355 - MMP_RS00110 0.60 +2.4 26,368 - MMP_RS00110 0.62 +0.5 26,412 - MMP_RS00110 0.71 +0.5 26,413 + MMP_RS00110 0.72 -0.7 26,564 - -1.5 26,587 - -2.9 26,587 - +0.9 26,587 - +1.1 26,600 - -0.4 26,600 - +1.0 26,600 - -1.9 26,600 - -1.0 26,600 - +1.3 26,600 - -0.9 26,601 + -1.3 26,601 + -1.3 26,601 + -1.6 26,601 + -2.4 26,601 + -2.1 26,601 + -1.8 26,602 - +0.1 26,602 - +0.4 26,602 - -0.6 26,602 - -0.5 26,602 - -1.0 26,602 - +0.1 26,603 + +0.7 26,603 + +1.2 26,603 + +0.7 26,603 + -0.2 26,603 + -3.1 26,604 - -1.3 26,604 - +0.9 26,604 - -1.3 26,604 - -1.1 26,604 - -0.5 26,604 - +0.2 26,605 + +0.4 26,605 + +0.3 26,605 + -2.7 26,605 + +0.9 26,605 + -0.5 26,605 + -0.3 26,605 + +0.1 26,607 + -0.5 26,635 - -0.3 26,654 + +1.6 26,654 + -3.3 26,660 - +1.8 26,660 - +1.8 26,660 - +0.7 26,661 + +0.2 26,665 + -2.2 26,665 + +0.2 26,665 + +0.2 26,666 - +1.1 26,667 + -0.7 26,670 + -2.0 26,670 + -2.3 26,670 + +0.0 26,671 - +0.8 26,672 + -1.9 26,672 + -0.7 26,702 - -0.2 26,750 - -0.0 26,751 + -3.7 26,751 + +0.6 26,774 - +0.9 26,774 - -0.3 26,794 - MMP_RS00115 0.11 -0.1 26,794 - MMP_RS00115 0.11 +0.3 26,829 - MMP_RS00115 0.15 -1.1 26,852 - MMP_RS00115 0.17 +0.7 26,855 + MMP_RS00115 0.17 -1.2 26,856 - MMP_RS00115 0.17 -1.6 26,856 - MMP_RS00115 0.17 +0.1 26,856 - MMP_RS00115 0.17 -3.8 26,856 - MMP_RS00115 0.17 +2.7 26,856 - MMP_RS00115 0.17 -1.9 26,857 + MMP_RS00115 0.17 -2.1 26,857 + MMP_RS00115 0.17 -0.5 26,857 + MMP_RS00115 0.17 +0.5 26,857 + MMP_RS00115 0.17 -1.2 26,915 + MMP_RS00115 0.23 -2.3 26,939 - MMP_RS00115 0.25 -0.0 26,940 + MMP_RS00115 0.25 -0.1 26,944 + MMP_RS00115 0.26 +1.7 26,981 - MMP_RS00115 0.29 +0.3 26,987 - MMP_RS00115 0.30 -0.9
Or see this region's nucleotide sequence