Experiment: ALP
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0500 and Dsui_0501 are separated by 5 nucleotides Dsui_0501 and Dsui_0502 are separated by 34 nucleotides Dsui_0502 and Dsui_0503 are separated by 46 nucleotides
Dsui_0500: Dsui_0500 - (p)ppGpp synthetase, RelA/SpoT family, at 501,253 to 503,484
_0500
Dsui_0501: Dsui_0501 - glycine/D-amino acid oxidase, deaminating, at 503,490 to 504,740
_0501
Dsui_0502: Dsui_0502 - enoyl-CoA hydratase/carnithine racemase, at 504,775 to 505,566
_0502
Dsui_0503: Dsui_0503 - Mg-dependent DNase, at 505,613 to 506,473
_0503
Position (kb) 503
504
505 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 502.502 kb on - strand, within Dsui_0500 at 502.502 kb on - strand, within Dsui_0500 at 503.002 kb on + strand, within Dsui_0500 at 503.066 kb on + strand, within Dsui_0500 at 504.780 kb on - strand at 504.780 kb on - strand at 504.808 kb on + strand at 504.828 kb on - strand at 504.830 kb on + strand at 504.945 kb on + strand, within Dsui_0502 at 505.044 kb on + strand, within Dsui_0502 at 505.052 kb on - strand, within Dsui_0502 at 505.060 kb on - strand, within Dsui_0502 at 505.244 kb on + strand, within Dsui_0502 at 505.325 kb on - strand, within Dsui_0502 at 505.340 kb on - strand at 505.407 kb on - strand, within Dsui_0502 at 505.410 kb on + strand, within Dsui_0502 at 505.410 kb on + strand, within Dsui_0502 at 505.618 kb on + strand at 505.713 kb on - strand at 505.713 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP remove 502,502 - Dsui_0500 0.56 -2.0 502,502 - Dsui_0500 0.56 +0.6 503,002 + Dsui_0500 0.78 -3.9 503,066 + Dsui_0500 0.81 -2.0 504,780 - -1.3 504,780 - +0.3 504,808 + -0.4 504,828 - -0.3 504,830 + -0.2 504,945 + Dsui_0502 0.21 -0.3 505,044 + Dsui_0502 0.34 -0.0 505,052 - Dsui_0502 0.35 +0.3 505,060 - Dsui_0502 0.36 -1.3 505,244 + Dsui_0502 0.59 -0.3 505,325 - Dsui_0502 0.69 -3.3 505,340 - +0.3 505,407 - Dsui_0502 0.80 -1.3 505,410 + Dsui_0502 0.80 -0.1 505,410 + Dsui_0502 0.80 +0.1 505,618 + -0.1 505,713 - -0.7 505,713 - +0.3
Or see this region's nucleotide sequence