Experiment: Casamino Acids (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1971 and BT1972 are separated by 230 nucleotides BT1972 and BT1973 are separated by 331 nucleotides
BT1971: BT1971 - catalase (NCBI ptt file), at 2,476,610 to 2,478,076
BT1971
BT1972: BT1972 - phosphoenolpyruvate synthase/pyruvate phosphate dikinase (NCBI ptt file), at 2,478,307 to 2,481,279
BT1972
BT1973: BT1973 - NAD-specific glutamate dehydrogenase (NCBI ptt file), at 2,481,611 to 2,482,948
BT1973
Position (kb)
2478
2479
2480
2481
2482 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 2477.452 kb on - strand, within BT1971 at 2477.565 kb on + strand, within BT1971 at 2477.566 kb on - strand, within BT1971 at 2477.699 kb on + strand, within BT1971 at 2477.835 kb on + strand, within BT1971 at 2477.837 kb on + strand, within BT1971 at 2477.837 kb on + strand, within BT1971 at 2477.838 kb on - strand, within BT1971 at 2477.843 kb on + strand, within BT1971 at 2477.858 kb on + strand, within BT1971 at 2477.870 kb on + strand, within BT1971 at 2477.870 kb on + strand, within BT1971 at 2478.110 kb on + strand at 2478.127 kb on - strand at 2478.151 kb on + strand at 2478.151 kb on + strand at 2478.164 kb on - strand at 2478.218 kb on + strand at 2478.219 kb on - strand at 2478.348 kb on + strand at 2478.349 kb on - strand at 2478.447 kb on - strand at 2478.447 kb on - strand at 2478.477 kb on + strand at 2478.482 kb on + strand at 2478.483 kb on - strand at 2478.483 kb on - strand at 2478.659 kb on + strand, within BT1972 at 2478.659 kb on + strand, within BT1972 at 2478.660 kb on - strand, within BT1972 at 2478.660 kb on - strand, within BT1972 at 2478.762 kb on + strand, within BT1972 at 2478.896 kb on + strand, within BT1972 at 2478.903 kb on + strand, within BT1972 at 2478.903 kb on + strand, within BT1972 at 2478.912 kb on + strand, within BT1972 at 2478.980 kb on + strand, within BT1972 at 2479.019 kb on - strand, within BT1972 at 2479.122 kb on - strand, within BT1972 at 2479.256 kb on - strand, within BT1972 at 2479.436 kb on + strand, within BT1972 at 2479.515 kb on + strand, within BT1972 at 2479.524 kb on + strand, within BT1972 at 2479.524 kb on + strand, within BT1972 at 2479.527 kb on - strand, within BT1972 at 2479.559 kb on + strand, within BT1972 at 2479.560 kb on - strand, within BT1972 at 2479.616 kb on + strand, within BT1972 at 2479.627 kb on - strand, within BT1972 at 2479.628 kb on + strand, within BT1972 at 2479.628 kb on + strand, within BT1972 at 2479.629 kb on - strand, within BT1972 at 2479.629 kb on - strand, within BT1972 at 2479.633 kb on - strand, within BT1972 at 2479.633 kb on - strand, within BT1972 at 2479.633 kb on - strand, within BT1972 at 2479.637 kb on + strand, within BT1972 at 2479.739 kb on - strand, within BT1972 at 2479.772 kb on + strand, within BT1972 at 2479.824 kb on + strand, within BT1972 at 2479.864 kb on + strand, within BT1972 at 2479.932 kb on + strand, within BT1972 at 2479.932 kb on + strand, within BT1972 at 2479.936 kb on - strand, within BT1972 at 2480.003 kb on + strand, within BT1972 at 2480.171 kb on + strand, within BT1972 at 2480.172 kb on - strand, within BT1972 at 2480.203 kb on - strand, within BT1972 at 2480.377 kb on - strand, within BT1972 at 2480.382 kb on + strand, within BT1972 at 2480.396 kb on + strand, within BT1972 at 2480.408 kb on + strand, within BT1972 at 2480.409 kb on - strand, within BT1972 at 2480.409 kb on - strand, within BT1972 at 2480.409 kb on - strand, within BT1972 at 2480.554 kb on + strand, within BT1972 at 2480.554 kb on + strand, within BT1972 at 2480.555 kb on - strand, within BT1972 at 2480.557 kb on + strand, within BT1972 at 2480.637 kb on + strand, within BT1972 at 2480.708 kb on + strand, within BT1972 at 2480.761 kb on + strand, within BT1972 at 2480.762 kb on - strand, within BT1972 at 2480.808 kb on + strand, within BT1972 at 2480.808 kb on + strand, within BT1972 at 2480.808 kb on + strand, within BT1972 at 2480.830 kb on - strand, within BT1972 at 2480.871 kb on - strand, within BT1972 at 2480.913 kb on + strand, within BT1972 at 2480.914 kb on - strand, within BT1972 at 2481.039 kb on + strand at 2481.039 kb on + strand at 2481.040 kb on - strand at 2481.117 kb on + strand at 2481.117 kb on + strand at 2481.117 kb on + strand at 2481.118 kb on - strand at 2481.126 kb on + strand at 2481.127 kb on - strand at 2481.127 kb on - strand at 2481.176 kb on + strand at 2481.280 kb on - strand at 2481.304 kb on + strand at 2481.329 kb on + strand at 2481.329 kb on + strand at 2481.362 kb on - strand at 2481.402 kb on - strand at 2481.402 kb on - strand at 2481.408 kb on + strand at 2481.408 kb on + strand at 2481.409 kb on - strand at 2481.416 kb on - strand at 2481.419 kb on + strand at 2481.426 kb on + strand at 2481.430 kb on - strand at 2481.454 kb on + strand at 2481.454 kb on + strand at 2481.471 kb on + strand at 2481.478 kb on + strand at 2481.516 kb on - strand at 2481.524 kb on - strand at 2481.524 kb on - strand at 2481.603 kb on - strand at 2481.607 kb on + strand at 2481.607 kb on + strand at 2481.663 kb on + strand at 2481.710 kb on - strand at 2481.711 kb on + strand at 2481.714 kb on - strand at 2481.750 kb on + strand, within BT1973 at 2481.751 kb on - strand, within BT1973 at 2481.775 kb on + strand, within BT1973 at 2481.776 kb on - strand, within BT1973 at 2481.776 kb on - strand, within BT1973 at 2481.836 kb on + strand, within BT1973 at 2481.843 kb on + strand, within BT1973 at 2481.843 kb on + strand, within BT1973 at 2482.089 kb on + strand, within BT1973 at 2482.089 kb on + strand, within BT1973 at 2482.090 kb on - strand, within BT1973 at 2482.132 kb on + strand, within BT1973 at 2482.132 kb on + strand, within BT1973 at 2482.132 kb on + strand, within BT1973 at 2482.132 kb on + strand, within BT1973 at 2482.132 kb on + strand, within BT1973 at 2482.133 kb on - strand, within BT1973 at 2482.133 kb on - strand, within BT1973 at 2482.133 kb on - strand, within BT1973 at 2482.133 kb on - strand, within BT1973 at 2482.145 kb on + strand, within BT1973 at 2482.146 kb on - strand, within BT1973 at 2482.146 kb on - strand, within BT1973 at 2482.146 kb on - strand, within BT1973 at 2482.247 kb on + strand, within BT1973
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino Acids (N) remove 2,477,452 - BT1971 0.57 -2.2 2,477,565 + BT1971 0.65 -1.6 2,477,566 - BT1971 0.65 -0.3 2,477,699 + BT1971 0.74 -1.9 2,477,835 + BT1971 0.84 +0.4 2,477,837 + BT1971 0.84 -0.7 2,477,837 + BT1971 0.84 -0.6 2,477,838 - BT1971 0.84 -2.4 2,477,843 + BT1971 0.84 -0.2 2,477,858 + BT1971 0.85 +0.3 2,477,870 + BT1971 0.86 -2.2 2,477,870 + BT1971 0.86 -0.2 2,478,110 + -0.8 2,478,127 - -1.7 2,478,151 + -0.4 2,478,151 + -0.2 2,478,164 - -0.9 2,478,218 + -1.7 2,478,219 - -0.9 2,478,348 + -1.2 2,478,349 - -0.2 2,478,447 - -3.4 2,478,447 - -2.4 2,478,477 + -0.6 2,478,482 + -4.3 2,478,483 - -4.3 2,478,483 - -2.4 2,478,659 + BT1972 0.12 -0.2 2,478,659 + BT1972 0.12 -5.4 2,478,660 - BT1972 0.12 -2.8 2,478,660 - BT1972 0.12 -3.4 2,478,762 + BT1972 0.15 -3.2 2,478,896 + BT1972 0.20 -2.2 2,478,903 + BT1972 0.20 -1.2 2,478,903 + BT1972 0.20 -2.2 2,478,912 + BT1972 0.20 -2.4 2,478,980 + BT1972 0.23 -4.6 2,479,019 - BT1972 0.24 -5.1 2,479,122 - BT1972 0.27 -2.6 2,479,256 - BT1972 0.32 -5.2 2,479,436 + BT1972 0.38 -0.9 2,479,515 + BT1972 0.41 -3.1 2,479,524 + BT1972 0.41 -0.6 2,479,524 + BT1972 0.41 -0.6 2,479,527 - BT1972 0.41 -4.5 2,479,559 + BT1972 0.42 -3.9 2,479,560 - BT1972 0.42 -1.6 2,479,616 + BT1972 0.44 -5.4 2,479,627 - BT1972 0.44 -1.2 2,479,628 + BT1972 0.44 -3.5 2,479,628 + BT1972 0.44 -4.7 2,479,629 - BT1972 0.44 -0.9 2,479,629 - BT1972 0.44 -0.9 2,479,633 - BT1972 0.45 -2.7 2,479,633 - BT1972 0.45 -2.2 2,479,633 - BT1972 0.45 -5.7 2,479,637 + BT1972 0.45 -3.8 2,479,739 - BT1972 0.48 -2.2 2,479,772 + BT1972 0.49 -2.2 2,479,824 + BT1972 0.51 -3.8 2,479,864 + BT1972 0.52 -0.9 2,479,932 + BT1972 0.55 -2.9 2,479,932 + BT1972 0.55 -1.2 2,479,936 - BT1972 0.55 -0.6 2,480,003 + BT1972 0.57 -4.8 2,480,171 + BT1972 0.63 -2.5 2,480,172 - BT1972 0.63 -1.2 2,480,203 - BT1972 0.64 -5.0 2,480,377 - BT1972 0.70 -3.2 2,480,382 + BT1972 0.70 -1.2 2,480,396 + BT1972 0.70 -3.9 2,480,408 + BT1972 0.71 -1.9 2,480,409 - BT1972 0.71 -0.2 2,480,409 - BT1972 0.71 -2.3 2,480,409 - BT1972 0.71 -5.2 2,480,554 + BT1972 0.76 -0.4 2,480,554 + BT1972 0.76 -4.0 2,480,555 - BT1972 0.76 -0.7 2,480,557 + BT1972 0.76 -1.2 2,480,637 + BT1972 0.78 -0.2 2,480,708 + BT1972 0.81 -2.3 2,480,761 + BT1972 0.83 -2.3 2,480,762 - BT1972 0.83 -0.4 2,480,808 + BT1972 0.84 -1.2 2,480,808 + BT1972 0.84 -1.6 2,480,808 + BT1972 0.84 -1.2 2,480,830 - BT1972 0.85 -0.5 2,480,871 - BT1972 0.86 -3.5 2,480,913 + BT1972 0.88 -4.4 2,480,914 - BT1972 0.88 -1.4 2,481,039 + -1.1 2,481,039 + -3.1 2,481,040 - +0.4 2,481,117 + -4.3 2,481,117 + -3.0 2,481,117 + -0.6 2,481,118 - -2.9 2,481,126 + -2.7 2,481,127 - -2.3 2,481,127 - -3.9 2,481,176 + -3.4 2,481,280 - +1.4 2,481,304 + -5.4 2,481,329 + -4.5 2,481,329 + -0.6 2,481,362 - -0.1 2,481,402 - -1.2 2,481,402 - +0.4 2,481,408 + -1.0 2,481,408 + +0.9 2,481,409 - -0.6 2,481,416 - -1.0 2,481,419 + -1.4 2,481,426 + -0.6 2,481,430 - -1.8 2,481,454 + +0.2 2,481,454 + +0.4 2,481,471 + -1.3 2,481,478 + -0.1 2,481,516 - -0.1 2,481,524 - -2.3 2,481,524 - -1.8 2,481,603 - +0.1 2,481,607 + +1.4 2,481,607 + -3.3 2,481,663 + -0.0 2,481,710 - +0.2 2,481,711 + -0.7 2,481,714 - +0.4 2,481,750 + BT1973 0.10 -1.3 2,481,751 - BT1973 0.10 +2.4 2,481,775 + BT1973 0.12 -0.9 2,481,776 - BT1973 0.12 -0.4 2,481,776 - BT1973 0.12 -1.6 2,481,836 + BT1973 0.17 -1.6 2,481,843 + BT1973 0.17 +0.4 2,481,843 + BT1973 0.17 +0.9 2,482,089 + BT1973 0.36 -0.7 2,482,089 + BT1973 0.36 -0.6 2,482,090 - BT1973 0.36 -1.4 2,482,132 + BT1973 0.39 -0.9 2,482,132 + BT1973 0.39 -0.3 2,482,132 + BT1973 0.39 +0.1 2,482,132 + BT1973 0.39 +0.3 2,482,132 + BT1973 0.39 -0.4 2,482,133 - BT1973 0.39 -1.2 2,482,133 - BT1973 0.39 -0.5 2,482,133 - BT1973 0.39 +0.3 2,482,133 - BT1973 0.39 -1.3 2,482,145 + BT1973 0.40 -1.4 2,482,146 - BT1973 0.40 -1.7 2,482,146 - BT1973 0.40 +0.4 2,482,146 - BT1973 0.40 -0.5 2,482,247 + BT1973 0.48 -2.7
Or see this region's nucleotide sequence