Experiment: LB 20C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5975 and Pf1N1B4_5976 are separated by 115 nucleotides Pf1N1B4_5976 and Pf1N1B4_5977 are separated by 71 nucleotides
Pf1N1B4_5975 - 2-methylaconitate racemase, at 422,673 to 423,701
_5975
Pf1N1B4_5976 - 1-pyrroline-4-hydroxy-2-carboxylate deaminase (EC 3.5.4.22), at 423,817 to 424,734
_5976
Pf1N1B4_5977 - Transporter, MFS superfamily, at 424,806 to 426,203
_5977
Position (kb) 423
424
425 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 423.335 kb on + strand at 423.335 kb on + strand, within Pf1N1B4_5975 at 423.336 kb on - strand, within Pf1N1B4_5975 at 423.373 kb on + strand, within Pf1N1B4_5975 at 423.374 kb on - strand, within Pf1N1B4_5975 at 423.589 kb on - strand, within Pf1N1B4_5975 at 423.589 kb on - strand, within Pf1N1B4_5975 at 423.739 kb on - strand at 423.739 kb on - strand at 423.831 kb on + strand at 423.832 kb on - strand at 424.192 kb on + strand, within Pf1N1B4_5976 at 424.228 kb on + strand at 424.228 kb on + strand, within Pf1N1B4_5976 at 424.246 kb on + strand, within Pf1N1B4_5976 at 424.528 kb on + strand, within Pf1N1B4_5976 at 424.529 kb on - strand, within Pf1N1B4_5976 at 424.820 kb on - strand at 424.862 kb on - strand at 425.598 kb on + strand, within Pf1N1B4_5977 at 425.599 kb on - strand at 425.599 kb on - strand, within Pf1N1B4_5977 at 425.625 kb on + strand, within Pf1N1B4_5977 at 425.721 kb on + strand, within Pf1N1B4_5977
Per-strain Table
Position Strand Gene LocusTag Fraction LB 20C remove 423,335 + -0.0 423,335 + Pf1N1B4_5975 0.64 +0.6 423,336 - Pf1N1B4_5975 0.64 -0.4 423,373 + Pf1N1B4_5975 0.68 -0.5 423,374 - Pf1N1B4_5975 0.68 -0.7 423,589 - Pf1N1B4_5975 0.89 +0.2 423,589 - Pf1N1B4_5975 0.89 -1.0 423,739 - -1.5 423,739 - -1.6 423,831 + -0.9 423,832 - -0.1 424,192 + Pf1N1B4_5976 0.41 -0.9 424,228 + +0.3 424,228 + Pf1N1B4_5976 0.45 -0.8 424,246 + Pf1N1B4_5976 0.47 +0.1 424,528 + Pf1N1B4_5976 0.77 -0.5 424,529 - Pf1N1B4_5976 0.78 -0.4 424,820 - -1.7 424,862 - -0.0 425,598 + Pf1N1B4_5977 0.57 -2.9 425,599 - +1.0 425,599 - Pf1N1B4_5977 0.57 -0.5 425,625 + Pf1N1B4_5977 0.59 -0.6 425,721 + Pf1N1B4_5977 0.65 -0.4
Or see this region's nucleotide sequence