Experiment: LB 20C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5813 and Pf1N1B4_5814 are separated by 60 nucleotides Pf1N1B4_5814 and Pf1N1B4_5815 are separated by 15 nucleotides
Pf1N1B4_5813 - 4-hydroxy-tetrahydrodipicolinate synthase (EC 4.3.3.7), at 245,881 to 246,756
_5813
Pf1N1B4_5814 - putative membrane protein, at 246,817 to 247,203
_5814
Pf1N1B4_5815 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 247,219 to 248,796
_5815
Position (kb) 246
247
248 Strain fitness (log2 ratio) -2
-1
0
1
2 at 245.834 kb on + strand at 245.834 kb on + strand at 245.834 kb on + strand at 245.834 kb on + strand at 245.834 kb on + strand at 245.835 kb on - strand at 245.898 kb on + strand at 245.899 kb on - strand at 245.899 kb on - strand at 245.899 kb on - strand at 246.197 kb on - strand, within Pf1N1B4_5813 at 246.199 kb on + strand, within Pf1N1B4_5813 at 246.199 kb on + strand, within Pf1N1B4_5813 at 246.458 kb on + strand, within Pf1N1B4_5813 at 246.582 kb on - strand, within Pf1N1B4_5813 at 246.755 kb on - strand at 246.797 kb on + strand at 247.057 kb on + strand, within Pf1N1B4_5814 at 247.057 kb on + strand, within Pf1N1B4_5814 at 247.058 kb on - strand, within Pf1N1B4_5814 at 247.058 kb on - strand, within Pf1N1B4_5814 at 247.147 kb on + strand, within Pf1N1B4_5814 at 247.148 kb on - strand, within Pf1N1B4_5814 at 247.148 kb on - strand, within Pf1N1B4_5814 at 247.265 kb on + strand at 247.265 kb on + strand at 247.266 kb on - strand at 247.734 kb on + strand, within Pf1N1B4_5815 at 247.785 kb on + strand, within Pf1N1B4_5815 at 247.788 kb on + strand, within Pf1N1B4_5815 at 247.789 kb on - strand, within Pf1N1B4_5815 at 248.186 kb on + strand, within Pf1N1B4_5815 at 248.186 kb on + strand at 248.187 kb on - strand, within Pf1N1B4_5815
Per-strain Table
Position Strand Gene LocusTag Fraction LB 20C remove 245,834 + -0.6 245,834 + -1.4 245,834 + +0.5 245,834 + -0.8 245,834 + -0.6 245,835 - -2.0 245,898 + +0.4 245,899 - -0.6 245,899 - +1.6 245,899 - +0.1 246,197 - Pf1N1B4_5813 0.36 -0.4 246,199 + Pf1N1B4_5813 0.36 -0.5 246,199 + Pf1N1B4_5813 0.36 -1.4 246,458 + Pf1N1B4_5813 0.66 -0.6 246,582 - Pf1N1B4_5813 0.80 -0.3 246,755 - +1.7 246,797 + -0.4 247,057 + Pf1N1B4_5814 0.62 -1.2 247,057 + Pf1N1B4_5814 0.62 -0.2 247,058 - Pf1N1B4_5814 0.62 +1.8 247,058 - Pf1N1B4_5814 0.62 +1.3 247,147 + Pf1N1B4_5814 0.85 -0.1 247,148 - Pf1N1B4_5814 0.86 -1.4 247,148 - Pf1N1B4_5814 0.86 -1.6 247,265 + +0.1 247,265 + -0.6 247,266 - -0.0 247,734 + Pf1N1B4_5815 0.33 -0.5 247,785 + Pf1N1B4_5815 0.36 +0.2 247,788 + Pf1N1B4_5815 0.36 -0.4 247,789 - Pf1N1B4_5815 0.36 -0.5 248,186 + Pf1N1B4_5815 0.61 +0.2 248,186 + +0.9 248,187 - Pf1N1B4_5815 0.61 -0.4
Or see this region's nucleotide sequence