Experiment: LB 20C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4890 and Pf1N1B4_4891 are separated by 122 nucleotides Pf1N1B4_4891 and Pf1N1B4_4892 are separated by 277 nucleotides Pf1N1B4_4892 and Pf1N1B4_4893 are separated by 51 nucleotides
Pf1N1B4_4890 - Cystathionine gamma-lyase (EC 4.4.1.1), at 5,412,449 to 5,413,630
_4890
Pf1N1B4_4891 - FIG00959288: hypothetical protein, at 5,413,753 to 5,414,028
_4891
Pf1N1B4_4892 - Transcriptional regulator, AraC family, at 5,414,306 to 5,415,121
_4892
Pf1N1B4_4893 - O-acetylhomoserine sulfhydrylase (EC 2.5.1.49), at 5,415,173 to 5,416,579
_4893
Position (kb)
5414
5415
5416 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 5413.761 kb on - strand at 5414.306 kb on - strand at 5414.432 kb on + strand at 5414.432 kb on + strand, within Pf1N1B4_4892 at 5414.483 kb on + strand, within Pf1N1B4_4892 at 5414.483 kb on + strand, within Pf1N1B4_4892 at 5414.540 kb on - strand, within Pf1N1B4_4892 at 5414.810 kb on + strand, within Pf1N1B4_4892 at 5414.880 kb on - strand, within Pf1N1B4_4892 at 5414.913 kb on - strand, within Pf1N1B4_4892 at 5414.919 kb on - strand, within Pf1N1B4_4892 at 5415.033 kb on - strand, within Pf1N1B4_4892 at 5415.308 kb on + strand at 5415.308 kb on + strand at 5415.311 kb on + strand at 5415.311 kb on + strand at 5415.311 kb on + strand at 5415.710 kb on - strand, within Pf1N1B4_4893 at 5415.713 kb on + strand at 5415.713 kb on + strand, within Pf1N1B4_4893 at 5415.713 kb on + strand, within Pf1N1B4_4893 at 5415.713 kb on + strand, within Pf1N1B4_4893 at 5415.714 kb on - strand, within Pf1N1B4_4893 at 5415.714 kb on - strand, within Pf1N1B4_4893 at 5415.797 kb on + strand at 5416.040 kb on + strand, within Pf1N1B4_4893 at 5416.041 kb on - strand at 5416.041 kb on - strand, within Pf1N1B4_4893 at 5416.041 kb on - strand, within Pf1N1B4_4893 at 5416.041 kb on - strand at 5416.071 kb on + strand, within Pf1N1B4_4893
Per-strain Table
Position Strand Gene LocusTag Fraction LB 20C remove 5,413,761 - -1.9 5,414,306 - +0.5 5,414,432 + +1.4 5,414,432 + Pf1N1B4_4892 0.15 +0.7 5,414,483 + Pf1N1B4_4892 0.22 -0.2 5,414,483 + Pf1N1B4_4892 0.22 -0.3 5,414,540 - Pf1N1B4_4892 0.29 -0.5 5,414,810 + Pf1N1B4_4892 0.62 -0.9 5,414,880 - Pf1N1B4_4892 0.70 -2.7 5,414,913 - Pf1N1B4_4892 0.74 -1.6 5,414,919 - Pf1N1B4_4892 0.75 -1.4 5,415,033 - Pf1N1B4_4892 0.89 +0.2 5,415,308 + -1.5 5,415,308 + -0.4 5,415,311 + -1.4 5,415,311 + -1.6 5,415,311 + -0.0 5,415,710 - Pf1N1B4_4893 0.38 -0.3 5,415,713 + +1.7 5,415,713 + Pf1N1B4_4893 0.38 +0.4 5,415,713 + Pf1N1B4_4893 0.38 +0.0 5,415,713 + Pf1N1B4_4893 0.38 +0.0 5,415,714 - Pf1N1B4_4893 0.38 +0.4 5,415,714 - Pf1N1B4_4893 0.38 +0.6 5,415,797 + +1.0 5,416,040 + Pf1N1B4_4893 0.62 +0.3 5,416,041 - +0.2 5,416,041 - Pf1N1B4_4893 0.62 +1.4 5,416,041 - Pf1N1B4_4893 0.62 +0.5 5,416,041 - +3.0 5,416,071 + Pf1N1B4_4893 0.64 -0.1
Or see this region's nucleotide sequence