Experiment: LB 20C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4838 and Pf1N1B4_4839 are separated by 12 nucleotides Pf1N1B4_4839 and Pf1N1B4_4840 are separated by 1 nucleotides Pf1N1B4_4840 and Pf1N1B4_4841 are separated by 685 nucleotides
Pf1N1B4_4838 - Putative lipase, at 5,343,703 to 5,345,832
_4838
Pf1N1B4_4839 - lipoprotein, at 5,345,845 to 5,346,639
_4839
Pf1N1B4_4840 - hypothetical protein, at 5,346,641 to 5,346,766
_4840
Pf1N1B4_4841 - hypothetical protein, at 5,347,452 to 5,348,303
_4841
Position (kb)
5345
5346
5347 Strain fitness (log2 ratio)
-2
-1
0
1 at 5345.122 kb on - strand at 5345.186 kb on + strand, within Pf1N1B4_4838 at 5345.335 kb on + strand, within Pf1N1B4_4838 at 5345.589 kb on - strand, within Pf1N1B4_4838 at 5345.917 kb on - strand at 5346.297 kb on - strand, within Pf1N1B4_4839 at 5346.332 kb on - strand, within Pf1N1B4_4839 at 5346.485 kb on + strand, within Pf1N1B4_4839 at 5346.486 kb on - strand at 5346.544 kb on - strand, within Pf1N1B4_4839 at 5346.812 kb on - strand at 5346.852 kb on - strand at 5347.080 kb on - strand at 5347.080 kb on - strand at 5347.101 kb on + strand at 5347.264 kb on + strand at 5347.264 kb on + strand at 5347.290 kb on + strand at 5347.291 kb on - strand at 5347.291 kb on - strand at 5347.474 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 20C remove 5,345,122 - +0.4 5,345,186 + Pf1N1B4_4838 0.70 -0.4 5,345,335 + Pf1N1B4_4838 0.77 -0.2 5,345,589 - Pf1N1B4_4838 0.89 +1.0 5,345,917 - -1.0 5,346,297 - Pf1N1B4_4839 0.57 -0.5 5,346,332 - Pf1N1B4_4839 0.61 -0.4 5,346,485 + Pf1N1B4_4839 0.81 -2.1 5,346,486 - -1.2 5,346,544 - Pf1N1B4_4839 0.88 -0.2 5,346,812 - +0.3 5,346,852 - +0.6 5,347,080 - -0.2 5,347,080 - +1.3 5,347,101 + +0.4 5,347,264 + -0.0 5,347,264 + -0.9 5,347,290 + -0.2 5,347,291 - -0.3 5,347,291 - -0.8 5,347,474 - -0.1
Or see this region's nucleotide sequence