Experiment: LB 20C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3231 and Pf1N1B4_3232 are separated by 283 nucleotides Pf1N1B4_3232 and Pf1N1B4_3233 overlap by 1 nucleotides Pf1N1B4_3233 and Pf1N1B4_3234 overlap by 1 nucleotides
Pf1N1B4_3231 - 4-hydroxybenzoate transporter, at 3,617,619 to 3,618,965
_3231
Pf1N1B4_3232 - 3-oxoadipate CoA-transferase subunit A (EC 2.8.3.6); Glutaconate CoA-transferase subunit A (EC 2.8.3.12), at 3,619,249 to 3,620,106
_3232
Pf1N1B4_3233 - 3-oxoadipate CoA-transferase subunit B (EC 2.8.3.6); Glutaconate CoA-transferase subunit B (EC 2.8.3.12), at 3,620,106 to 3,620,885
_3233
Pf1N1B4_3234 - Beta-ketoadipyl CoA thiolase (EC 2.3.1.-), at 3,620,885 to 3,622,087
_3234
Position (kb)
3619
3620
3621 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3618.329 kb on + strand, within Pf1N1B4_3231 at 3618.329 kb on + strand, within Pf1N1B4_3231 at 3618.329 kb on + strand, within Pf1N1B4_3231 at 3618.330 kb on - strand, within Pf1N1B4_3231 at 3618.330 kb on - strand, within Pf1N1B4_3231 at 3618.330 kb on - strand, within Pf1N1B4_3231 at 3618.399 kb on + strand at 3618.399 kb on + strand, within Pf1N1B4_3231 at 3618.399 kb on + strand, within Pf1N1B4_3231 at 3618.399 kb on + strand, within Pf1N1B4_3231 at 3618.399 kb on + strand, within Pf1N1B4_3231 at 3618.399 kb on + strand, within Pf1N1B4_3231 at 3618.399 kb on + strand, within Pf1N1B4_3231 at 3618.432 kb on + strand, within Pf1N1B4_3231 at 3618.432 kb on + strand, within Pf1N1B4_3231 at 3618.432 kb on + strand, within Pf1N1B4_3231 at 3618.432 kb on + strand, within Pf1N1B4_3231 at 3618.432 kb on + strand, within Pf1N1B4_3231 at 3618.432 kb on + strand, within Pf1N1B4_3231 at 3618.432 kb on + strand, within Pf1N1B4_3231 at 3618.433 kb on - strand, within Pf1N1B4_3231 at 3618.433 kb on - strand, within Pf1N1B4_3231 at 3618.433 kb on - strand, within Pf1N1B4_3231 at 3618.433 kb on - strand, within Pf1N1B4_3231 at 3618.433 kb on - strand, within Pf1N1B4_3231 at 3618.456 kb on + strand, within Pf1N1B4_3231 at 3618.457 kb on - strand, within Pf1N1B4_3231 at 3618.625 kb on + strand, within Pf1N1B4_3231 at 3618.625 kb on + strand, within Pf1N1B4_3231 at 3618.625 kb on + strand, within Pf1N1B4_3231 at 3618.626 kb on - strand, within Pf1N1B4_3231 at 3618.646 kb on - strand, within Pf1N1B4_3231 at 3618.759 kb on + strand, within Pf1N1B4_3231 at 3619.170 kb on + strand at 3619.212 kb on - strand at 3619.228 kb on - strand at 3619.417 kb on + strand, within Pf1N1B4_3232 at 3619.418 kb on - strand, within Pf1N1B4_3232 at 3619.418 kb on - strand, within Pf1N1B4_3232 at 3619.418 kb on - strand, within Pf1N1B4_3232 at 3619.579 kb on + strand, within Pf1N1B4_3232 at 3619.580 kb on - strand, within Pf1N1B4_3232 at 3619.580 kb on - strand, within Pf1N1B4_3232 at 3619.624 kb on + strand, within Pf1N1B4_3232 at 3619.920 kb on - strand, within Pf1N1B4_3232 at 3619.978 kb on + strand, within Pf1N1B4_3232 at 3619.978 kb on + strand, within Pf1N1B4_3232 at 3619.978 kb on + strand at 3619.979 kb on - strand, within Pf1N1B4_3232 at 3619.979 kb on - strand, within Pf1N1B4_3232 at 3620.038 kb on + strand at 3620.038 kb on + strand at 3620.038 kb on + strand at 3620.038 kb on + strand at 3620.038 kb on + strand at 3620.038 kb on + strand at 3620.038 kb on + strand at 3620.039 kb on - strand at 3620.039 kb on - strand at 3620.039 kb on - strand at 3620.039 kb on - strand at 3620.104 kb on + strand at 3620.104 kb on + strand at 3620.104 kb on + strand at 3620.105 kb on - strand at 3620.105 kb on - strand at 3620.105 kb on - strand at 3620.112 kb on + strand at 3620.112 kb on + strand at 3620.112 kb on + strand at 3620.113 kb on - strand at 3620.113 kb on - strand at 3620.113 kb on - strand at 3620.113 kb on - strand at 3620.113 kb on - strand at 3620.113 kb on - strand at 3620.113 kb on - strand at 3620.239 kb on + strand, within Pf1N1B4_3233 at 3620.239 kb on + strand at 3620.239 kb on + strand, within Pf1N1B4_3233 at 3620.239 kb on + strand, within Pf1N1B4_3233 at 3620.240 kb on - strand, within Pf1N1B4_3233 at 3620.240 kb on - strand, within Pf1N1B4_3233 at 3620.240 kb on - strand, within Pf1N1B4_3233 at 3620.250 kb on + strand, within Pf1N1B4_3233 at 3620.250 kb on + strand, within Pf1N1B4_3233 at 3620.250 kb on + strand, within Pf1N1B4_3233 at 3620.250 kb on + strand, within Pf1N1B4_3233 at 3620.250 kb on + strand, within Pf1N1B4_3233 at 3620.251 kb on - strand, within Pf1N1B4_3233 at 3620.251 kb on - strand, within Pf1N1B4_3233 at 3620.251 kb on - strand, within Pf1N1B4_3233 at 3620.284 kb on + strand, within Pf1N1B4_3233 at 3620.455 kb on - strand, within Pf1N1B4_3233 at 3620.455 kb on - strand, within Pf1N1B4_3233 at 3620.524 kb on + strand, within Pf1N1B4_3233 at 3620.525 kb on - strand, within Pf1N1B4_3233 at 3620.897 kb on + strand at 3620.897 kb on + strand at 3620.897 kb on + strand at 3620.897 kb on + strand at 3620.897 kb on + strand at 3620.897 kb on + strand at 3620.897 kb on + strand at 3620.897 kb on + strand at 3620.898 kb on - strand at 3620.898 kb on - strand at 3620.898 kb on - strand at 3620.898 kb on - strand at 3620.899 kb on + strand at 3620.899 kb on + strand at 3620.899 kb on + strand at 3620.899 kb on + strand at 3620.899 kb on + strand at 3620.899 kb on + strand at 3620.900 kb on - strand at 3620.900 kb on - strand at 3620.900 kb on - strand at 3621.084 kb on + strand, within Pf1N1B4_3234 at 3621.084 kb on + strand, within Pf1N1B4_3234 at 3621.084 kb on + strand, within Pf1N1B4_3234 at 3621.085 kb on - strand, within Pf1N1B4_3234
Per-strain Table
Position Strand Gene LocusTag Fraction LB 20C remove 3,618,329 + Pf1N1B4_3231 0.53 -0.3 3,618,329 + Pf1N1B4_3231 0.53 -0.9 3,618,329 + Pf1N1B4_3231 0.53 +2.0 3,618,330 - Pf1N1B4_3231 0.53 +0.1 3,618,330 - Pf1N1B4_3231 0.53 -0.7 3,618,330 - Pf1N1B4_3231 0.53 -2.0 3,618,399 + -0.0 3,618,399 + Pf1N1B4_3231 0.58 +0.1 3,618,399 + Pf1N1B4_3231 0.58 -1.0 3,618,399 + Pf1N1B4_3231 0.58 +0.1 3,618,399 + Pf1N1B4_3231 0.58 +1.3 3,618,399 + Pf1N1B4_3231 0.58 -0.5 3,618,399 + Pf1N1B4_3231 0.58 +1.4 3,618,432 + Pf1N1B4_3231 0.60 +0.5 3,618,432 + Pf1N1B4_3231 0.60 -0.5 3,618,432 + Pf1N1B4_3231 0.60 -0.2 3,618,432 + Pf1N1B4_3231 0.60 +0.2 3,618,432 + Pf1N1B4_3231 0.60 +1.1 3,618,432 + Pf1N1B4_3231 0.60 +0.8 3,618,432 + Pf1N1B4_3231 0.60 -0.7 3,618,433 - Pf1N1B4_3231 0.60 -1.3 3,618,433 - Pf1N1B4_3231 0.60 -0.2 3,618,433 - Pf1N1B4_3231 0.60 -1.0 3,618,433 - Pf1N1B4_3231 0.60 -0.4 3,618,433 - Pf1N1B4_3231 0.60 -0.2 3,618,456 + Pf1N1B4_3231 0.62 -0.3 3,618,457 - Pf1N1B4_3231 0.62 -0.4 3,618,625 + Pf1N1B4_3231 0.75 -0.5 3,618,625 + Pf1N1B4_3231 0.75 +0.2 3,618,625 + Pf1N1B4_3231 0.75 +1.4 3,618,626 - Pf1N1B4_3231 0.75 -0.3 3,618,646 - Pf1N1B4_3231 0.76 -0.3 3,618,759 + Pf1N1B4_3231 0.85 -0.4 3,619,170 + -0.6 3,619,212 - -0.4 3,619,228 - -0.5 3,619,417 + Pf1N1B4_3232 0.20 -0.6 3,619,418 - Pf1N1B4_3232 0.20 -0.7 3,619,418 - Pf1N1B4_3232 0.20 -2.0 3,619,418 - Pf1N1B4_3232 0.20 -0.9 3,619,579 + Pf1N1B4_3232 0.38 +0.3 3,619,580 - Pf1N1B4_3232 0.39 -0.6 3,619,580 - Pf1N1B4_3232 0.39 -0.6 3,619,624 + Pf1N1B4_3232 0.44 -0.5 3,619,920 - Pf1N1B4_3232 0.78 -1.2 3,619,978 + Pf1N1B4_3232 0.85 -0.8 3,619,978 + Pf1N1B4_3232 0.85 -0.2 3,619,978 + -1.0 3,619,979 - Pf1N1B4_3232 0.85 +0.9 3,619,979 - Pf1N1B4_3232 0.85 +0.7 3,620,038 + +1.3 3,620,038 + +0.2 3,620,038 + -0.1 3,620,038 + +0.3 3,620,038 + -0.9 3,620,038 + -0.1 3,620,038 + +0.1 3,620,039 - -1.0 3,620,039 - -0.9 3,620,039 - +0.8 3,620,039 - -0.1 3,620,104 + -0.4 3,620,104 + +0.6 3,620,104 + -0.2 3,620,105 - -1.4 3,620,105 - -0.5 3,620,105 - -0.0 3,620,112 + +0.4 3,620,112 + -1.0 3,620,112 + -1.2 3,620,113 - -1.4 3,620,113 - -0.7 3,620,113 - +1.1 3,620,113 - +0.7 3,620,113 - +0.6 3,620,113 - -0.7 3,620,113 - +0.2 3,620,239 + Pf1N1B4_3233 0.17 -1.7 3,620,239 + +0.8 3,620,239 + Pf1N1B4_3233 0.17 -1.9 3,620,239 + Pf1N1B4_3233 0.17 +0.6 3,620,240 - Pf1N1B4_3233 0.17 -0.1 3,620,240 - Pf1N1B4_3233 0.17 -0.0 3,620,240 - Pf1N1B4_3233 0.17 -0.5 3,620,250 + Pf1N1B4_3233 0.18 -1.4 3,620,250 + Pf1N1B4_3233 0.18 -1.4 3,620,250 + Pf1N1B4_3233 0.18 +0.4 3,620,250 + Pf1N1B4_3233 0.18 +0.2 3,620,250 + Pf1N1B4_3233 0.18 -1.7 3,620,251 - Pf1N1B4_3233 0.19 -0.4 3,620,251 - Pf1N1B4_3233 0.19 -0.1 3,620,251 - Pf1N1B4_3233 0.19 -1.1 3,620,284 + Pf1N1B4_3233 0.23 +1.0 3,620,455 - Pf1N1B4_3233 0.45 -0.2 3,620,455 - Pf1N1B4_3233 0.45 -0.0 3,620,524 + Pf1N1B4_3233 0.54 -0.5 3,620,525 - Pf1N1B4_3233 0.54 -1.1 3,620,897 + +0.1 3,620,897 + +0.8 3,620,897 + -1.0 3,620,897 + -0.9 3,620,897 + +0.2 3,620,897 + +0.8 3,620,897 + -0.7 3,620,897 + +0.1 3,620,898 - -0.1 3,620,898 - +0.5 3,620,898 - +0.7 3,620,898 - -0.3 3,620,899 + +0.5 3,620,899 + -1.1 3,620,899 + -1.7 3,620,899 + -0.3 3,620,899 + +0.4 3,620,899 + -0.5 3,620,900 - -0.4 3,620,900 - -0.8 3,620,900 - -0.1 3,621,084 + Pf1N1B4_3234 0.17 +0.3 3,621,084 + Pf1N1B4_3234 0.17 +0.5 3,621,084 + Pf1N1B4_3234 0.17 -1.8 3,621,085 - Pf1N1B4_3234 0.17 -0.3
Or see this region's nucleotide sequence