Experiment: LB 20C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2836 and Pf1N1B4_2837 are separated by 63 nucleotides Pf1N1B4_2837 and Pf1N1B4_2838 are separated by 9 nucleotides Pf1N1B4_2838 and Pf1N1B4_2839 overlap by 4 nucleotides Pf1N1B4_2839 and Pf1N1B4_2840 are separated by 8 nucleotides
Pf1N1B4_2836 - FIG028593: membrane protein, at 3,182,602 to 3,183,291
_2836
Pf1N1B4_2837 - FIG00956650: hypothetical protein, at 3,183,355 to 3,183,570
_2837
Pf1N1B4_2838 - FIG003276: zinc-binding protein, at 3,183,580 to 3,183,780
_2838
Pf1N1B4_2839 - Dephospho-CoA kinase (EC 2.7.1.24), at 3,183,777 to 3,184,388
_2839
Pf1N1B4_2840 - Leader peptidase (Prepilin peptidase) (EC 3.4.23.43) / N-methyltransferase (EC 2.1.1.-), at 3,184,397 to 3,185,269
_2840
Position (kb)
3183
3184 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3182.618 kb on + strand at 3182.618 kb on + strand at 3182.619 kb on - strand at 3182.619 kb on - strand at 3182.619 kb on - strand at 3182.656 kb on + strand at 3182.656 kb on + strand at 3182.656 kb on + strand at 3182.656 kb on + strand at 3182.657 kb on - strand at 3182.657 kb on - strand at 3182.657 kb on - strand at 3182.657 kb on - strand at 3182.657 kb on - strand at 3182.657 kb on - strand at 3182.660 kb on + strand at 3182.732 kb on + strand, within Pf1N1B4_2836 at 3182.732 kb on + strand, within Pf1N1B4_2836 at 3182.732 kb on + strand, within Pf1N1B4_2836 at 3182.732 kb on + strand, within Pf1N1B4_2836 at 3182.732 kb on + strand, within Pf1N1B4_2836 at 3182.732 kb on + strand at 3182.732 kb on + strand, within Pf1N1B4_2836 at 3182.733 kb on - strand, within Pf1N1B4_2836 at 3182.733 kb on - strand, within Pf1N1B4_2836 at 3182.733 kb on - strand, within Pf1N1B4_2836 at 3182.733 kb on - strand, within Pf1N1B4_2836 at 3182.733 kb on - strand, within Pf1N1B4_2836 at 3182.733 kb on - strand, within Pf1N1B4_2836 at 3182.733 kb on - strand, within Pf1N1B4_2836 at 3182.733 kb on - strand, within Pf1N1B4_2836 at 3182.733 kb on - strand, within Pf1N1B4_2836 at 3182.733 kb on - strand at 3182.733 kb on - strand, within Pf1N1B4_2836 at 3182.733 kb on - strand, within Pf1N1B4_2836 at 3182.733 kb on - strand, within Pf1N1B4_2836 at 3182.733 kb on - strand, within Pf1N1B4_2836 at 3182.803 kb on + strand, within Pf1N1B4_2836 at 3182.804 kb on - strand, within Pf1N1B4_2836 at 3182.804 kb on - strand, within Pf1N1B4_2836 at 3182.804 kb on - strand, within Pf1N1B4_2836 at 3182.804 kb on - strand, within Pf1N1B4_2836 at 3182.804 kb on - strand, within Pf1N1B4_2836 at 3183.019 kb on + strand, within Pf1N1B4_2836 at 3183.019 kb on + strand, within Pf1N1B4_2836 at 3183.019 kb on + strand, within Pf1N1B4_2836 at 3183.019 kb on + strand, within Pf1N1B4_2836 at 3183.019 kb on + strand, within Pf1N1B4_2836 at 3183.019 kb on + strand, within Pf1N1B4_2836 at 3183.019 kb on + strand, within Pf1N1B4_2836 at 3183.019 kb on + strand, within Pf1N1B4_2836 at 3183.019 kb on + strand, within Pf1N1B4_2836 at 3183.019 kb on + strand, within Pf1N1B4_2836 at 3183.019 kb on + strand, within Pf1N1B4_2836 at 3183.019 kb on + strand, within Pf1N1B4_2836 at 3183.020 kb on - strand, within Pf1N1B4_2836 at 3183.020 kb on - strand, within Pf1N1B4_2836 at 3183.020 kb on - strand, within Pf1N1B4_2836 at 3183.020 kb on - strand, within Pf1N1B4_2836 at 3183.020 kb on - strand, within Pf1N1B4_2836 at 3183.020 kb on - strand at 3183.020 kb on - strand, within Pf1N1B4_2836 at 3183.020 kb on - strand, within Pf1N1B4_2836 at 3183.020 kb on - strand, within Pf1N1B4_2836 at 3183.020 kb on - strand, within Pf1N1B4_2836 at 3183.020 kb on - strand, within Pf1N1B4_2836 at 3183.020 kb on - strand, within Pf1N1B4_2836 at 3183.020 kb on - strand, within Pf1N1B4_2836 at 3183.020 kb on - strand, within Pf1N1B4_2836 at 3183.020 kb on - strand, within Pf1N1B4_2836 at 3183.021 kb on + strand, within Pf1N1B4_2836 at 3183.021 kb on + strand, within Pf1N1B4_2836 at 3183.021 kb on + strand, within Pf1N1B4_2836 at 3183.021 kb on + strand, within Pf1N1B4_2836 at 3183.021 kb on + strand, within Pf1N1B4_2836 at 3183.021 kb on + strand, within Pf1N1B4_2836 at 3183.021 kb on + strand, within Pf1N1B4_2836 at 3183.021 kb on + strand, within Pf1N1B4_2836 at 3183.097 kb on + strand, within Pf1N1B4_2836 at 3183.097 kb on + strand, within Pf1N1B4_2836 at 3183.098 kb on - strand, within Pf1N1B4_2836 at 3183.145 kb on + strand, within Pf1N1B4_2836 at 3183.155 kb on + strand, within Pf1N1B4_2836 at 3183.178 kb on - strand, within Pf1N1B4_2836 at 3183.364 kb on + strand at 3183.364 kb on + strand at 3183.364 kb on + strand at 3183.364 kb on + strand at 3183.364 kb on + strand at 3183.365 kb on - strand at 3183.365 kb on - strand at 3183.365 kb on - strand at 3183.489 kb on + strand, within Pf1N1B4_2837 at 3183.489 kb on + strand, within Pf1N1B4_2837 at 3183.489 kb on + strand, within Pf1N1B4_2837 at 3183.489 kb on + strand, within Pf1N1B4_2837 at 3183.489 kb on + strand, within Pf1N1B4_2837 at 3183.490 kb on - strand, within Pf1N1B4_2837 at 3183.490 kb on - strand, within Pf1N1B4_2837 at 3183.491 kb on + strand, within Pf1N1B4_2837 at 3183.491 kb on + strand, within Pf1N1B4_2837 at 3183.491 kb on + strand, within Pf1N1B4_2837 at 3183.491 kb on + strand, within Pf1N1B4_2837 at 3183.491 kb on + strand, within Pf1N1B4_2837 at 3183.491 kb on + strand, within Pf1N1B4_2837 at 3183.491 kb on + strand, within Pf1N1B4_2837 at 3183.491 kb on + strand, within Pf1N1B4_2837 at 3183.491 kb on + strand, within Pf1N1B4_2837 at 3183.491 kb on + strand, within Pf1N1B4_2837 at 3183.492 kb on - strand, within Pf1N1B4_2837 at 3183.492 kb on - strand, within Pf1N1B4_2837 at 3183.492 kb on - strand, within Pf1N1B4_2837 at 3183.492 kb on - strand, within Pf1N1B4_2837 at 3183.492 kb on - strand, within Pf1N1B4_2837 at 3183.492 kb on - strand, within Pf1N1B4_2837 at 3183.492 kb on - strand, within Pf1N1B4_2837 at 3183.492 kb on - strand, within Pf1N1B4_2837 at 3183.492 kb on - strand, within Pf1N1B4_2837 at 3183.492 kb on - strand, within Pf1N1B4_2837 at 3183.492 kb on - strand at 3183.492 kb on - strand, within Pf1N1B4_2837 at 3183.492 kb on - strand, within Pf1N1B4_2837 at 3183.492 kb on - strand, within Pf1N1B4_2837 at 3183.492 kb on - strand, within Pf1N1B4_2837 at 3183.492 kb on - strand, within Pf1N1B4_2837 at 3183.550 kb on + strand at 3183.551 kb on - strand at 3183.558 kb on + strand at 3183.800 kb on + strand at 3183.800 kb on + strand at 3183.800 kb on + strand at 3183.800 kb on + strand at 3183.800 kb on + strand at 3183.801 kb on - strand at 3183.801 kb on - strand at 3183.801 kb on - strand at 3183.801 kb on - strand at 3183.801 kb on - strand at 3183.801 kb on - strand at 3183.801 kb on - strand at 3183.801 kb on - strand at 3183.801 kb on - strand at 3183.801 kb on - strand at 3184.395 kb on - strand at 3184.423 kb on - strand at 3184.423 kb on - strand at 3184.423 kb on - strand at 3184.423 kb on - strand at 3184.423 kb on - strand at 3184.434 kb on - strand at 3184.434 kb on - strand at 3184.480 kb on - strand at 3184.673 kb on + strand, within Pf1N1B4_2840
Per-strain Table
Position Strand Gene LocusTag Fraction LB 20C remove 3,182,618 + +0.3 3,182,618 + -1.3 3,182,619 - +0.3 3,182,619 - +0.6 3,182,619 - -0.1 3,182,656 + -2.2 3,182,656 + -0.1 3,182,656 + +0.3 3,182,656 + -0.3 3,182,657 - -0.9 3,182,657 - +0.1 3,182,657 - -0.2 3,182,657 - -0.5 3,182,657 - +1.3 3,182,657 - -0.4 3,182,660 + +0.2 3,182,732 + Pf1N1B4_2836 0.19 +0.1 3,182,732 + Pf1N1B4_2836 0.19 +0.6 3,182,732 + Pf1N1B4_2836 0.19 -0.4 3,182,732 + Pf1N1B4_2836 0.19 -0.1 3,182,732 + Pf1N1B4_2836 0.19 -0.9 3,182,732 + -0.4 3,182,732 + Pf1N1B4_2836 0.19 -0.2 3,182,733 - Pf1N1B4_2836 0.19 -0.4 3,182,733 - Pf1N1B4_2836 0.19 -0.9 3,182,733 - Pf1N1B4_2836 0.19 -0.8 3,182,733 - Pf1N1B4_2836 0.19 -0.4 3,182,733 - Pf1N1B4_2836 0.19 -0.7 3,182,733 - Pf1N1B4_2836 0.19 -0.3 3,182,733 - Pf1N1B4_2836 0.19 -0.4 3,182,733 - Pf1N1B4_2836 0.19 +0.5 3,182,733 - Pf1N1B4_2836 0.19 +0.1 3,182,733 - -1.2 3,182,733 - Pf1N1B4_2836 0.19 -0.1 3,182,733 - Pf1N1B4_2836 0.19 +1.4 3,182,733 - Pf1N1B4_2836 0.19 -0.2 3,182,733 - Pf1N1B4_2836 0.19 +0.8 3,182,803 + Pf1N1B4_2836 0.29 +0.4 3,182,804 - Pf1N1B4_2836 0.29 -1.3 3,182,804 - Pf1N1B4_2836 0.29 +0.6 3,182,804 - Pf1N1B4_2836 0.29 -0.2 3,182,804 - Pf1N1B4_2836 0.29 +0.0 3,182,804 - Pf1N1B4_2836 0.29 -0.1 3,183,019 + Pf1N1B4_2836 0.60 -2.2 3,183,019 + Pf1N1B4_2836 0.60 -0.7 3,183,019 + Pf1N1B4_2836 0.60 -0.9 3,183,019 + Pf1N1B4_2836 0.60 +1.0 3,183,019 + Pf1N1B4_2836 0.60 -0.2 3,183,019 + Pf1N1B4_2836 0.60 -0.1 3,183,019 + Pf1N1B4_2836 0.60 -0.6 3,183,019 + Pf1N1B4_2836 0.60 -0.8 3,183,019 + Pf1N1B4_2836 0.60 -1.8 3,183,019 + Pf1N1B4_2836 0.60 -0.2 3,183,019 + Pf1N1B4_2836 0.60 -0.2 3,183,019 + Pf1N1B4_2836 0.60 +2.2 3,183,020 - Pf1N1B4_2836 0.61 +0.8 3,183,020 - Pf1N1B4_2836 0.61 -0.4 3,183,020 - Pf1N1B4_2836 0.61 -0.2 3,183,020 - Pf1N1B4_2836 0.61 +0.2 3,183,020 - Pf1N1B4_2836 0.61 +0.9 3,183,020 - -0.7 3,183,020 - Pf1N1B4_2836 0.61 +0.5 3,183,020 - Pf1N1B4_2836 0.61 -0.4 3,183,020 - Pf1N1B4_2836 0.61 -0.2 3,183,020 - Pf1N1B4_2836 0.61 +0.3 3,183,020 - Pf1N1B4_2836 0.61 -0.3 3,183,020 - Pf1N1B4_2836 0.61 -0.4 3,183,020 - Pf1N1B4_2836 0.61 +0.7 3,183,020 - Pf1N1B4_2836 0.61 +0.4 3,183,020 - Pf1N1B4_2836 0.61 +0.1 3,183,021 + Pf1N1B4_2836 0.61 +0.2 3,183,021 + Pf1N1B4_2836 0.61 +0.3 3,183,021 + Pf1N1B4_2836 0.61 -0.0 3,183,021 + Pf1N1B4_2836 0.61 -0.3 3,183,021 + Pf1N1B4_2836 0.61 +0.2 3,183,021 + Pf1N1B4_2836 0.61 +0.7 3,183,021 + Pf1N1B4_2836 0.61 -1.2 3,183,021 + Pf1N1B4_2836 0.61 +0.0 3,183,097 + Pf1N1B4_2836 0.72 -1.3 3,183,097 + Pf1N1B4_2836 0.72 +0.4 3,183,098 - Pf1N1B4_2836 0.72 +1.1 3,183,145 + Pf1N1B4_2836 0.79 +0.7 3,183,155 + Pf1N1B4_2836 0.80 -0.3 3,183,178 - Pf1N1B4_2836 0.83 +0.3 3,183,364 + -0.7 3,183,364 + -1.2 3,183,364 + -1.5 3,183,364 + -1.9 3,183,364 + -0.9 3,183,365 - -0.9 3,183,365 - +0.1 3,183,365 - -0.4 3,183,489 + Pf1N1B4_2837 0.62 -0.1 3,183,489 + Pf1N1B4_2837 0.62 +0.0 3,183,489 + Pf1N1B4_2837 0.62 +1.0 3,183,489 + Pf1N1B4_2837 0.62 -0.3 3,183,489 + Pf1N1B4_2837 0.62 -0.0 3,183,490 - Pf1N1B4_2837 0.62 +0.6 3,183,490 - Pf1N1B4_2837 0.62 +0.1 3,183,491 + Pf1N1B4_2837 0.63 -1.5 3,183,491 + Pf1N1B4_2837 0.63 -1.3 3,183,491 + Pf1N1B4_2837 0.63 -0.9 3,183,491 + Pf1N1B4_2837 0.63 +1.2 3,183,491 + Pf1N1B4_2837 0.63 +0.0 3,183,491 + Pf1N1B4_2837 0.63 -0.0 3,183,491 + Pf1N1B4_2837 0.63 +0.6 3,183,491 + Pf1N1B4_2837 0.63 -0.0 3,183,491 + Pf1N1B4_2837 0.63 +0.1 3,183,491 + Pf1N1B4_2837 0.63 +0.4 3,183,492 - Pf1N1B4_2837 0.63 +0.7 3,183,492 - Pf1N1B4_2837 0.63 -0.3 3,183,492 - Pf1N1B4_2837 0.63 -0.7 3,183,492 - Pf1N1B4_2837 0.63 -0.8 3,183,492 - Pf1N1B4_2837 0.63 +0.4 3,183,492 - Pf1N1B4_2837 0.63 -0.1 3,183,492 - Pf1N1B4_2837 0.63 +0.4 3,183,492 - Pf1N1B4_2837 0.63 -0.7 3,183,492 - Pf1N1B4_2837 0.63 +0.6 3,183,492 - Pf1N1B4_2837 0.63 +0.2 3,183,492 - -1.1 3,183,492 - Pf1N1B4_2837 0.63 +1.1 3,183,492 - Pf1N1B4_2837 0.63 +1.9 3,183,492 - Pf1N1B4_2837 0.63 +0.3 3,183,492 - Pf1N1B4_2837 0.63 -0.1 3,183,492 - Pf1N1B4_2837 0.63 -0.5 3,183,550 + +0.3 3,183,551 - +0.3 3,183,558 + +0.3 3,183,800 + +1.1 3,183,800 + +0.2 3,183,800 + -0.4 3,183,800 + +1.6 3,183,800 + -0.8 3,183,801 - +0.1 3,183,801 - -2.6 3,183,801 - +0.0 3,183,801 - -0.2 3,183,801 - -0.2 3,183,801 - -0.4 3,183,801 - +0.5 3,183,801 - -0.5 3,183,801 - +1.0 3,183,801 - -0.8 3,184,395 - -0.5 3,184,423 - -0.8 3,184,423 - +0.7 3,184,423 - -0.3 3,184,423 - +1.9 3,184,423 - -0.2 3,184,434 - +0.7 3,184,434 - -0.5 3,184,480 - -0.7 3,184,673 + Pf1N1B4_2840 0.32 -0.0
Or see this region's nucleotide sequence