Experiment: LB 20C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1640 and Pf1N1B4_1641 are separated by 13 nucleotides Pf1N1B4_1641 and Pf1N1B4_1642 are separated by 78 nucleotides Pf1N1B4_1642 and Pf1N1B4_1643 overlap by 4 nucleotides Pf1N1B4_1643 and Pf1N1B4_1644 are separated by 7 nucleotides
Pf1N1B4_1640 - Histidine ABC transporter, ATP-binding protein HisP (TC 3.A.1.3.1), at 1,883,799 to 1,884,572
_1640
Pf1N1B4_1641 - Lysine-arginine-ornithine-binding periplasmic protein precursor (TC 3.A.1.3.1), at 1,884,586 to 1,885,332
_1641
Pf1N1B4_1642 - Arginine ABC transporter, permease protein ArtQ, at 1,885,411 to 1,886,106
_1642
Pf1N1B4_1643 - Amino acid ABC transporter, permease protein, at 1,886,103 to 1,886,792
_1643
Pf1N1B4_1644 - SAM-dependent methyltransferases, at 1,886,800 to 1,888,002
_1644
Position (kb)
1885
1886
1887 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1884.595 kb on + strand at 1884.596 kb on - strand at 1884.596 kb on - strand at 1884.789 kb on + strand, within Pf1N1B4_1641 at 1884.789 kb on + strand, within Pf1N1B4_1641 at 1884.789 kb on + strand, within Pf1N1B4_1641 at 1884.790 kb on - strand, within Pf1N1B4_1641 at 1884.790 kb on - strand, within Pf1N1B4_1641 at 1884.904 kb on + strand, within Pf1N1B4_1641 at 1884.904 kb on + strand, within Pf1N1B4_1641 at 1884.904 kb on + strand, within Pf1N1B4_1641 at 1884.905 kb on - strand, within Pf1N1B4_1641 at 1884.905 kb on - strand, within Pf1N1B4_1641 at 1884.907 kb on + strand, within Pf1N1B4_1641 at 1884.908 kb on - strand, within Pf1N1B4_1641 at 1884.908 kb on - strand, within Pf1N1B4_1641 at 1884.908 kb on - strand, within Pf1N1B4_1641 at 1884.908 kb on - strand, within Pf1N1B4_1641 at 1885.057 kb on + strand, within Pf1N1B4_1641 at 1885.126 kb on + strand, within Pf1N1B4_1641 at 1885.126 kb on + strand, within Pf1N1B4_1641 at 1885.126 kb on + strand, within Pf1N1B4_1641 at 1885.126 kb on + strand, within Pf1N1B4_1641 at 1885.126 kb on + strand, within Pf1N1B4_1641 at 1885.126 kb on + strand, within Pf1N1B4_1641 at 1885.126 kb on + strand, within Pf1N1B4_1641 at 1885.126 kb on + strand, within Pf1N1B4_1641 at 1885.126 kb on + strand, within Pf1N1B4_1641 at 1885.126 kb on + strand, within Pf1N1B4_1641 at 1885.126 kb on + strand, within Pf1N1B4_1641 at 1885.126 kb on + strand, within Pf1N1B4_1641 at 1885.126 kb on + strand, within Pf1N1B4_1641 at 1885.127 kb on - strand, within Pf1N1B4_1641 at 1885.127 kb on - strand, within Pf1N1B4_1641 at 1885.127 kb on - strand, within Pf1N1B4_1641 at 1885.127 kb on - strand, within Pf1N1B4_1641 at 1885.127 kb on - strand, within Pf1N1B4_1641 at 1885.127 kb on - strand, within Pf1N1B4_1641 at 1885.127 kb on - strand, within Pf1N1B4_1641 at 1885.127 kb on - strand, within Pf1N1B4_1641 at 1885.127 kb on - strand, within Pf1N1B4_1641 at 1885.127 kb on - strand, within Pf1N1B4_1641 at 1885.135 kb on + strand, within Pf1N1B4_1641 at 1885.135 kb on + strand, within Pf1N1B4_1641 at 1885.135 kb on + strand, within Pf1N1B4_1641 at 1885.135 kb on + strand, within Pf1N1B4_1641 at 1885.135 kb on + strand, within Pf1N1B4_1641 at 1885.135 kb on + strand, within Pf1N1B4_1641 at 1885.135 kb on + strand, within Pf1N1B4_1641 at 1885.135 kb on + strand, within Pf1N1B4_1641 at 1885.136 kb on - strand, within Pf1N1B4_1641 at 1885.136 kb on - strand, within Pf1N1B4_1641 at 1885.136 kb on - strand, within Pf1N1B4_1641 at 1885.136 kb on - strand, within Pf1N1B4_1641 at 1885.168 kb on + strand, within Pf1N1B4_1641 at 1885.168 kb on + strand, within Pf1N1B4_1641 at 1885.168 kb on + strand, within Pf1N1B4_1641 at 1885.169 kb on - strand, within Pf1N1B4_1641 at 1885.288 kb on + strand at 1885.288 kb on + strand at 1885.288 kb on + strand at 1885.289 kb on - strand at 1885.289 kb on - strand at 1885.289 kb on - strand at 1885.309 kb on + strand at 1885.309 kb on + strand at 1885.309 kb on + strand at 1885.309 kb on + strand at 1885.309 kb on + strand at 1885.309 kb on + strand at 1885.309 kb on + strand at 1885.309 kb on + strand at 1885.310 kb on - strand at 1885.310 kb on - strand at 1885.310 kb on - strand at 1885.310 kb on - strand at 1885.327 kb on + strand at 1885.327 kb on + strand at 1885.327 kb on + strand at 1885.327 kb on + strand at 1885.327 kb on + strand at 1885.327 kb on + strand at 1885.327 kb on + strand at 1885.327 kb on + strand at 1885.327 kb on + strand at 1885.328 kb on - strand at 1885.328 kb on - strand at 1885.328 kb on - strand at 1885.328 kb on - strand at 1885.543 kb on + strand, within Pf1N1B4_1642 at 1885.573 kb on + strand, within Pf1N1B4_1642 at 1885.574 kb on - strand, within Pf1N1B4_1642 at 1885.624 kb on + strand, within Pf1N1B4_1642 at 1885.999 kb on + strand, within Pf1N1B4_1642 at 1885.999 kb on + strand, within Pf1N1B4_1642 at 1885.999 kb on + strand, within Pf1N1B4_1642 at 1885.999 kb on + strand, within Pf1N1B4_1642 at 1885.999 kb on + strand, within Pf1N1B4_1642 at 1885.999 kb on + strand, within Pf1N1B4_1642 at 1885.999 kb on + strand, within Pf1N1B4_1642 at 1885.999 kb on + strand, within Pf1N1B4_1642 at 1886.000 kb on - strand, within Pf1N1B4_1642 at 1886.000 kb on - strand, within Pf1N1B4_1642 at 1886.000 kb on - strand, within Pf1N1B4_1642 at 1886.000 kb on - strand, within Pf1N1B4_1642 at 1886.000 kb on - strand, within Pf1N1B4_1642 at 1886.001 kb on + strand, within Pf1N1B4_1642 at 1886.001 kb on + strand, within Pf1N1B4_1642 at 1886.002 kb on - strand, within Pf1N1B4_1642 at 1886.002 kb on - strand, within Pf1N1B4_1642 at 1886.003 kb on - strand, within Pf1N1B4_1642 at 1886.020 kb on + strand, within Pf1N1B4_1642 at 1886.021 kb on - strand, within Pf1N1B4_1642 at 1886.021 kb on - strand, within Pf1N1B4_1642 at 1886.021 kb on - strand, within Pf1N1B4_1642 at 1886.021 kb on - strand, within Pf1N1B4_1642 at 1886.021 kb on - strand, within Pf1N1B4_1642 at 1886.021 kb on - strand, within Pf1N1B4_1642 at 1886.021 kb on - strand, within Pf1N1B4_1642 at 1886.021 kb on - strand, within Pf1N1B4_1642 at 1886.061 kb on + strand at 1886.245 kb on - strand, within Pf1N1B4_1643 at 1886.245 kb on - strand, within Pf1N1B4_1643 at 1886.245 kb on - strand, within Pf1N1B4_1643 at 1886.245 kb on - strand, within Pf1N1B4_1643 at 1886.245 kb on - strand, within Pf1N1B4_1643 at 1886.245 kb on - strand, within Pf1N1B4_1643 at 1886.268 kb on + strand, within Pf1N1B4_1643 at 1886.268 kb on + strand, within Pf1N1B4_1643 at 1886.269 kb on - strand, within Pf1N1B4_1643 at 1886.269 kb on - strand, within Pf1N1B4_1643 at 1886.319 kb on + strand, within Pf1N1B4_1643 at 1886.319 kb on + strand, within Pf1N1B4_1643 at 1886.320 kb on - strand, within Pf1N1B4_1643 at 1886.320 kb on - strand, within Pf1N1B4_1643 at 1886.320 kb on - strand, within Pf1N1B4_1643 at 1886.320 kb on - strand, within Pf1N1B4_1643 at 1886.320 kb on - strand, within Pf1N1B4_1643 at 1886.322 kb on + strand, within Pf1N1B4_1643 at 1886.323 kb on - strand, within Pf1N1B4_1643 at 1886.323 kb on - strand, within Pf1N1B4_1643 at 1886.427 kb on + strand, within Pf1N1B4_1643 at 1886.427 kb on + strand, within Pf1N1B4_1643 at 1886.427 kb on + strand, within Pf1N1B4_1643 at 1886.428 kb on - strand at 1886.428 kb on - strand, within Pf1N1B4_1643 at 1886.428 kb on - strand, within Pf1N1B4_1643 at 1886.428 kb on - strand, within Pf1N1B4_1643 at 1886.526 kb on + strand, within Pf1N1B4_1643 at 1886.526 kb on + strand, within Pf1N1B4_1643 at 1886.526 kb on + strand, within Pf1N1B4_1643 at 1886.526 kb on + strand, within Pf1N1B4_1643 at 1886.526 kb on + strand, within Pf1N1B4_1643 at 1886.527 kb on - strand, within Pf1N1B4_1643 at 1886.527 kb on - strand, within Pf1N1B4_1643 at 1886.527 kb on - strand, within Pf1N1B4_1643 at 1886.568 kb on + strand, within Pf1N1B4_1643 at 1886.568 kb on + strand, within Pf1N1B4_1643 at 1886.670 kb on + strand, within Pf1N1B4_1643 at 1886.671 kb on - strand, within Pf1N1B4_1643 at 1886.709 kb on + strand, within Pf1N1B4_1643 at 1886.709 kb on + strand, within Pf1N1B4_1643 at 1886.709 kb on + strand, within Pf1N1B4_1643 at 1886.709 kb on + strand, within Pf1N1B4_1643 at 1886.709 kb on + strand, within Pf1N1B4_1643 at 1886.709 kb on + strand, within Pf1N1B4_1643 at 1886.709 kb on + strand, within Pf1N1B4_1643 at 1886.709 kb on + strand, within Pf1N1B4_1643 at 1886.709 kb on + strand, within Pf1N1B4_1643 at 1886.709 kb on + strand, within Pf1N1B4_1643 at 1886.710 kb on - strand, within Pf1N1B4_1643 at 1886.710 kb on - strand, within Pf1N1B4_1643 at 1886.710 kb on - strand, within Pf1N1B4_1643 at 1886.710 kb on - strand, within Pf1N1B4_1643 at 1886.710 kb on - strand, within Pf1N1B4_1643 at 1886.724 kb on + strand at 1886.725 kb on - strand at 1886.980 kb on - strand, within Pf1N1B4_1644 at 1886.980 kb on - strand, within Pf1N1B4_1644 at 1886.980 kb on - strand, within Pf1N1B4_1644 at 1887.062 kb on + strand, within Pf1N1B4_1644 at 1887.062 kb on + strand at 1887.072 kb on + strand, within Pf1N1B4_1644 at 1887.073 kb on - strand, within Pf1N1B4_1644
Per-strain Table
Position Strand Gene LocusTag Fraction LB 20C remove 1,884,595 + +0.7 1,884,596 - +0.4 1,884,596 - +0.2 1,884,789 + Pf1N1B4_1641 0.27 +0.3 1,884,789 + Pf1N1B4_1641 0.27 -1.2 1,884,789 + Pf1N1B4_1641 0.27 -0.6 1,884,790 - Pf1N1B4_1641 0.27 -0.1 1,884,790 - Pf1N1B4_1641 0.27 +1.3 1,884,904 + Pf1N1B4_1641 0.43 +0.7 1,884,904 + Pf1N1B4_1641 0.43 +0.0 1,884,904 + Pf1N1B4_1641 0.43 +1.1 1,884,905 - Pf1N1B4_1641 0.43 +0.4 1,884,905 - Pf1N1B4_1641 0.43 +0.4 1,884,907 + Pf1N1B4_1641 0.43 -0.4 1,884,908 - Pf1N1B4_1641 0.43 -0.1 1,884,908 - Pf1N1B4_1641 0.43 +0.0 1,884,908 - Pf1N1B4_1641 0.43 -0.6 1,884,908 - Pf1N1B4_1641 0.43 -0.5 1,885,057 + Pf1N1B4_1641 0.63 -1.1 1,885,126 + Pf1N1B4_1641 0.72 -0.4 1,885,126 + Pf1N1B4_1641 0.72 -0.5 1,885,126 + Pf1N1B4_1641 0.72 -1.1 1,885,126 + Pf1N1B4_1641 0.72 +1.3 1,885,126 + Pf1N1B4_1641 0.72 -0.1 1,885,126 + Pf1N1B4_1641 0.72 +0.2 1,885,126 + Pf1N1B4_1641 0.72 +0.1 1,885,126 + Pf1N1B4_1641 0.72 -0.8 1,885,126 + Pf1N1B4_1641 0.72 -0.7 1,885,126 + Pf1N1B4_1641 0.72 -0.9 1,885,126 + Pf1N1B4_1641 0.72 -0.8 1,885,126 + Pf1N1B4_1641 0.72 -0.1 1,885,126 + Pf1N1B4_1641 0.72 +0.8 1,885,127 - Pf1N1B4_1641 0.72 +0.5 1,885,127 - Pf1N1B4_1641 0.72 -0.3 1,885,127 - Pf1N1B4_1641 0.72 -0.4 1,885,127 - Pf1N1B4_1641 0.72 -0.1 1,885,127 - Pf1N1B4_1641 0.72 +1.0 1,885,127 - Pf1N1B4_1641 0.72 -1.7 1,885,127 - Pf1N1B4_1641 0.72 +0.4 1,885,127 - Pf1N1B4_1641 0.72 -1.2 1,885,127 - Pf1N1B4_1641 0.72 +0.1 1,885,127 - Pf1N1B4_1641 0.72 -0.8 1,885,135 + Pf1N1B4_1641 0.73 -0.3 1,885,135 + Pf1N1B4_1641 0.73 -0.1 1,885,135 + Pf1N1B4_1641 0.73 +0.3 1,885,135 + Pf1N1B4_1641 0.73 -0.0 1,885,135 + Pf1N1B4_1641 0.73 +0.5 1,885,135 + Pf1N1B4_1641 0.73 +0.6 1,885,135 + Pf1N1B4_1641 0.73 -0.2 1,885,135 + Pf1N1B4_1641 0.73 +0.2 1,885,136 - Pf1N1B4_1641 0.74 +0.2 1,885,136 - Pf1N1B4_1641 0.74 -0.7 1,885,136 - Pf1N1B4_1641 0.74 +0.1 1,885,136 - Pf1N1B4_1641 0.74 +1.2 1,885,168 + Pf1N1B4_1641 0.78 -0.5 1,885,168 + Pf1N1B4_1641 0.78 -0.2 1,885,168 + Pf1N1B4_1641 0.78 -0.9 1,885,169 - Pf1N1B4_1641 0.78 -2.0 1,885,288 + +0.6 1,885,288 + -1.1 1,885,288 + +0.1 1,885,289 - +1.6 1,885,289 - +0.1 1,885,289 - +0.2 1,885,309 + -0.1 1,885,309 + -1.0 1,885,309 + -0.0 1,885,309 + +0.6 1,885,309 + -1.3 1,885,309 + +0.5 1,885,309 + -0.6 1,885,309 + -0.5 1,885,310 - +0.1 1,885,310 - +0.6 1,885,310 - -0.9 1,885,310 - -0.6 1,885,327 + +0.2 1,885,327 + +0.6 1,885,327 + -0.3 1,885,327 + +0.7 1,885,327 + -0.4 1,885,327 + +0.6 1,885,327 + -0.4 1,885,327 + +1.0 1,885,327 + +1.4 1,885,328 - +0.9 1,885,328 - -1.0 1,885,328 - -0.0 1,885,328 - +0.2 1,885,543 + Pf1N1B4_1642 0.19 -0.2 1,885,573 + Pf1N1B4_1642 0.23 +0.1 1,885,574 - Pf1N1B4_1642 0.23 +1.1 1,885,624 + Pf1N1B4_1642 0.31 +0.8 1,885,999 + Pf1N1B4_1642 0.84 -0.7 1,885,999 + Pf1N1B4_1642 0.84 +1.1 1,885,999 + Pf1N1B4_1642 0.84 -0.9 1,885,999 + Pf1N1B4_1642 0.84 -0.4 1,885,999 + Pf1N1B4_1642 0.84 -1.0 1,885,999 + Pf1N1B4_1642 0.84 -1.0 1,885,999 + Pf1N1B4_1642 0.84 -0.3 1,885,999 + Pf1N1B4_1642 0.84 -0.6 1,886,000 - Pf1N1B4_1642 0.85 -1.0 1,886,000 - Pf1N1B4_1642 0.85 -0.4 1,886,000 - Pf1N1B4_1642 0.85 +1.1 1,886,000 - Pf1N1B4_1642 0.85 +1.0 1,886,000 - Pf1N1B4_1642 0.85 +0.3 1,886,001 + Pf1N1B4_1642 0.85 -0.4 1,886,001 + Pf1N1B4_1642 0.85 -2.2 1,886,002 - Pf1N1B4_1642 0.85 -1.3 1,886,002 - Pf1N1B4_1642 0.85 +1.1 1,886,003 - Pf1N1B4_1642 0.85 -0.3 1,886,020 + Pf1N1B4_1642 0.88 -2.3 1,886,021 - Pf1N1B4_1642 0.88 -0.2 1,886,021 - Pf1N1B4_1642 0.88 -0.2 1,886,021 - Pf1N1B4_1642 0.88 +0.1 1,886,021 - Pf1N1B4_1642 0.88 -0.5 1,886,021 - Pf1N1B4_1642 0.88 -0.6 1,886,021 - Pf1N1B4_1642 0.88 -1.1 1,886,021 - Pf1N1B4_1642 0.88 +0.0 1,886,021 - Pf1N1B4_1642 0.88 -0.7 1,886,061 + +0.1 1,886,245 - Pf1N1B4_1643 0.21 +1.0 1,886,245 - Pf1N1B4_1643 0.21 -0.4 1,886,245 - Pf1N1B4_1643 0.21 +0.1 1,886,245 - Pf1N1B4_1643 0.21 -0.5 1,886,245 - Pf1N1B4_1643 0.21 -0.1 1,886,245 - Pf1N1B4_1643 0.21 +0.3 1,886,268 + Pf1N1B4_1643 0.24 -1.2 1,886,268 + Pf1N1B4_1643 0.24 +0.1 1,886,269 - Pf1N1B4_1643 0.24 -0.1 1,886,269 - Pf1N1B4_1643 0.24 +1.0 1,886,319 + Pf1N1B4_1643 0.31 +0.7 1,886,319 + Pf1N1B4_1643 0.31 -0.5 1,886,320 - Pf1N1B4_1643 0.31 -0.4 1,886,320 - Pf1N1B4_1643 0.31 -0.6 1,886,320 - Pf1N1B4_1643 0.31 -0.5 1,886,320 - Pf1N1B4_1643 0.31 +1.3 1,886,320 - Pf1N1B4_1643 0.31 -1.6 1,886,322 + Pf1N1B4_1643 0.32 +0.1 1,886,323 - Pf1N1B4_1643 0.32 -0.2 1,886,323 - Pf1N1B4_1643 0.32 -0.2 1,886,427 + Pf1N1B4_1643 0.47 -1.4 1,886,427 + Pf1N1B4_1643 0.47 -0.0 1,886,427 + Pf1N1B4_1643 0.47 +0.2 1,886,428 - +2.8 1,886,428 - Pf1N1B4_1643 0.47 -1.0 1,886,428 - Pf1N1B4_1643 0.47 -0.7 1,886,428 - Pf1N1B4_1643 0.47 +0.7 1,886,526 + Pf1N1B4_1643 0.61 -0.4 1,886,526 + Pf1N1B4_1643 0.61 +0.3 1,886,526 + Pf1N1B4_1643 0.61 -1.2 1,886,526 + Pf1N1B4_1643 0.61 -0.4 1,886,526 + Pf1N1B4_1643 0.61 -0.4 1,886,527 - Pf1N1B4_1643 0.61 -1.2 1,886,527 - Pf1N1B4_1643 0.61 +0.3 1,886,527 - Pf1N1B4_1643 0.61 +0.7 1,886,568 + Pf1N1B4_1643 0.67 -0.4 1,886,568 + Pf1N1B4_1643 0.67 -0.2 1,886,670 + Pf1N1B4_1643 0.82 -2.3 1,886,671 - Pf1N1B4_1643 0.82 +0.3 1,886,709 + Pf1N1B4_1643 0.88 -1.3 1,886,709 + Pf1N1B4_1643 0.88 -0.9 1,886,709 + Pf1N1B4_1643 0.88 +0.7 1,886,709 + Pf1N1B4_1643 0.88 -0.4 1,886,709 + Pf1N1B4_1643 0.88 -0.6 1,886,709 + Pf1N1B4_1643 0.88 -0.5 1,886,709 + Pf1N1B4_1643 0.88 +0.6 1,886,709 + Pf1N1B4_1643 0.88 -1.4 1,886,709 + Pf1N1B4_1643 0.88 -1.1 1,886,709 + Pf1N1B4_1643 0.88 -0.8 1,886,710 - Pf1N1B4_1643 0.88 -0.2 1,886,710 - Pf1N1B4_1643 0.88 -0.3 1,886,710 - Pf1N1B4_1643 0.88 +0.0 1,886,710 - Pf1N1B4_1643 0.88 -0.4 1,886,710 - Pf1N1B4_1643 0.88 -0.2 1,886,724 + +0.1 1,886,725 - -0.9 1,886,980 - Pf1N1B4_1644 0.15 -0.9 1,886,980 - Pf1N1B4_1644 0.15 -0.8 1,886,980 - Pf1N1B4_1644 0.15 -0.6 1,887,062 + Pf1N1B4_1644 0.22 +1.4 1,887,062 + +0.1 1,887,072 + Pf1N1B4_1644 0.23 -1.0 1,887,073 - Pf1N1B4_1644 0.23 -0.7
Or see this region's nucleotide sequence