Experiment: LB 20C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1310 and Pf1N1B4_1311 are separated by 30 nucleotides Pf1N1B4_1311 and Pf1N1B4_1312 are separated by 83 nucleotides Pf1N1B4_1312 and Pf1N1B4_1313 overlap by 36 nucleotides
Pf1N1B4_1310 - Ribosomal protein L11 methyltransferase (EC 2.1.1.-), at 1,493,712 to 1,494,590
_1310
Pf1N1B4_1311 - Probable transmembrane protein, at 1,494,621 to 1,495,841
_1311
Pf1N1B4_1312 - hypothetical protein, at 1,495,925 to 1,496,062
_1312
Pf1N1B4_1313 - tRNA dihydrouridine synthase B (EC 1.-.-.-), at 1,496,027 to 1,497,037
_1313
Position (kb)
1494
1495
1496 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1493.651 kb on - strand at 1493.689 kb on + strand at 1493.689 kb on + strand at 1493.689 kb on + strand at 1493.689 kb on + strand at 1493.690 kb on - strand at 1493.691 kb on + strand at 1493.763 kb on + strand at 1493.763 kb on + strand at 1493.763 kb on + strand at 1493.764 kb on - strand at 1494.088 kb on + strand, within Pf1N1B4_1310 at 1494.089 kb on - strand, within Pf1N1B4_1310 at 1494.323 kb on + strand, within Pf1N1B4_1310 at 1494.323 kb on + strand, within Pf1N1B4_1310 at 1494.323 kb on + strand, within Pf1N1B4_1310 at 1494.323 kb on + strand, within Pf1N1B4_1310 at 1494.324 kb on - strand, within Pf1N1B4_1310 at 1494.324 kb on - strand, within Pf1N1B4_1310 at 1494.324 kb on - strand, within Pf1N1B4_1310 at 1494.324 kb on - strand, within Pf1N1B4_1310 at 1494.348 kb on + strand, within Pf1N1B4_1310 at 1494.349 kb on - strand, within Pf1N1B4_1310 at 1494.385 kb on + strand, within Pf1N1B4_1310 at 1494.386 kb on - strand, within Pf1N1B4_1310 at 1494.386 kb on - strand, within Pf1N1B4_1310 at 1494.386 kb on - strand, within Pf1N1B4_1310 at 1494.516 kb on + strand at 1494.573 kb on - strand at 1494.606 kb on - strand at 1494.681 kb on - strand at 1494.734 kb on - strand at 1494.754 kb on + strand, within Pf1N1B4_1311 at 1494.755 kb on - strand, within Pf1N1B4_1311 at 1494.755 kb on - strand, within Pf1N1B4_1311 at 1495.303 kb on + strand, within Pf1N1B4_1311 at 1495.304 kb on - strand, within Pf1N1B4_1311 at 1495.426 kb on - strand, within Pf1N1B4_1311 at 1495.435 kb on - strand, within Pf1N1B4_1311 at 1495.611 kb on + strand, within Pf1N1B4_1311 at 1495.611 kb on + strand, within Pf1N1B4_1311 at 1495.611 kb on + strand, within Pf1N1B4_1311 at 1495.611 kb on + strand, within Pf1N1B4_1311 at 1495.611 kb on + strand, within Pf1N1B4_1311 at 1495.612 kb on - strand, within Pf1N1B4_1311 at 1495.612 kb on - strand, within Pf1N1B4_1311 at 1495.612 kb on - strand, within Pf1N1B4_1311 at 1495.713 kb on + strand, within Pf1N1B4_1311 at 1495.812 kb on + strand at 1495.812 kb on + strand at 1495.812 kb on + strand at 1495.871 kb on + strand at 1495.890 kb on + strand at 1495.893 kb on + strand at 1495.893 kb on + strand at 1496.026 kb on + strand, within Pf1N1B4_1312 at 1496.026 kb on + strand, within Pf1N1B4_1312 at 1496.026 kb on + strand, within Pf1N1B4_1312 at 1496.026 kb on + strand, within Pf1N1B4_1312 at 1496.026 kb on + strand, within Pf1N1B4_1312 at 1496.026 kb on + strand, within Pf1N1B4_1312 at 1496.026 kb on + strand, within Pf1N1B4_1312 at 1496.026 kb on + strand, within Pf1N1B4_1312 at 1496.051 kb on + strand at 1496.051 kb on + strand at 1496.051 kb on + strand at 1496.051 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.145 kb on + strand, within Pf1N1B4_1313 at 1496.145 kb on + strand, within Pf1N1B4_1313 at 1496.145 kb on + strand, within Pf1N1B4_1313 at 1496.145 kb on + strand, within Pf1N1B4_1313 at 1496.145 kb on + strand, within Pf1N1B4_1313 at 1496.320 kb on + strand, within Pf1N1B4_1313 at 1496.564 kb on + strand, within Pf1N1B4_1313 at 1496.564 kb on + strand, within Pf1N1B4_1313 at 1496.564 kb on + strand, within Pf1N1B4_1313 at 1496.564 kb on + strand, within Pf1N1B4_1313 at 1496.564 kb on + strand, within Pf1N1B4_1313 at 1496.564 kb on + strand, within Pf1N1B4_1313 at 1496.564 kb on + strand, within Pf1N1B4_1313 at 1496.564 kb on + strand, within Pf1N1B4_1313 at 1496.582 kb on + strand, within Pf1N1B4_1313 at 1496.753 kb on + strand, within Pf1N1B4_1313 at 1496.753 kb on + strand, within Pf1N1B4_1313 at 1496.753 kb on + strand, within Pf1N1B4_1313
Per-strain Table
Position Strand Gene LocusTag Fraction LB 20C remove 1,493,651 - -0.2 1,493,689 + +0.4 1,493,689 + +0.7 1,493,689 + +0.0 1,493,689 + +0.9 1,493,690 - -1.7 1,493,691 + -0.8 1,493,763 + -1.5 1,493,763 + +0.3 1,493,763 + +0.1 1,493,764 - +1.7 1,494,088 + Pf1N1B4_1310 0.43 +1.1 1,494,089 - Pf1N1B4_1310 0.43 -0.4 1,494,323 + Pf1N1B4_1310 0.70 -0.2 1,494,323 + Pf1N1B4_1310 0.70 -0.3 1,494,323 + Pf1N1B4_1310 0.70 +0.0 1,494,323 + Pf1N1B4_1310 0.70 +0.0 1,494,324 - Pf1N1B4_1310 0.70 +0.7 1,494,324 - Pf1N1B4_1310 0.70 +0.4 1,494,324 - Pf1N1B4_1310 0.70 -0.6 1,494,324 - Pf1N1B4_1310 0.70 +0.1 1,494,348 + Pf1N1B4_1310 0.72 +0.0 1,494,349 - Pf1N1B4_1310 0.72 -0.5 1,494,385 + Pf1N1B4_1310 0.77 -1.9 1,494,386 - Pf1N1B4_1310 0.77 +1.2 1,494,386 - Pf1N1B4_1310 0.77 -0.6 1,494,386 - Pf1N1B4_1310 0.77 +1.8 1,494,516 + +0.9 1,494,573 - -1.7 1,494,606 - -1.5 1,494,681 - -1.0 1,494,734 - -0.8 1,494,754 + Pf1N1B4_1311 0.11 -0.9 1,494,755 - Pf1N1B4_1311 0.11 +0.2 1,494,755 - Pf1N1B4_1311 0.11 -1.8 1,495,303 + Pf1N1B4_1311 0.56 -1.7 1,495,304 - Pf1N1B4_1311 0.56 +0.4 1,495,426 - Pf1N1B4_1311 0.66 -2.2 1,495,435 - Pf1N1B4_1311 0.67 +0.8 1,495,611 + Pf1N1B4_1311 0.81 -0.4 1,495,611 + Pf1N1B4_1311 0.81 -1.5 1,495,611 + Pf1N1B4_1311 0.81 -1.7 1,495,611 + Pf1N1B4_1311 0.81 -0.8 1,495,611 + Pf1N1B4_1311 0.81 -1.3 1,495,612 - Pf1N1B4_1311 0.81 -0.8 1,495,612 - Pf1N1B4_1311 0.81 -1.2 1,495,612 - Pf1N1B4_1311 0.81 -0.2 1,495,713 + Pf1N1B4_1311 0.89 +1.3 1,495,812 + +0.0 1,495,812 + +0.6 1,495,812 + -0.7 1,495,871 + -0.3 1,495,890 + +0.7 1,495,893 + +0.6 1,495,893 + +0.2 1,496,026 + Pf1N1B4_1312 0.73 -1.3 1,496,026 + Pf1N1B4_1312 0.73 -0.5 1,496,026 + Pf1N1B4_1312 0.73 +0.2 1,496,026 + Pf1N1B4_1312 0.73 +0.7 1,496,026 + Pf1N1B4_1312 0.73 -0.2 1,496,026 + Pf1N1B4_1312 0.73 +0.6 1,496,026 + Pf1N1B4_1312 0.73 +0.7 1,496,026 + Pf1N1B4_1312 0.73 -0.4 1,496,051 + -0.2 1,496,051 + -0.9 1,496,051 + -0.6 1,496,051 + -0.0 1,496,053 + -0.0 1,496,053 + -2.0 1,496,053 + +0.1 1,496,053 + -1.0 1,496,053 + +0.0 1,496,053 + -0.6 1,496,053 + -1.2 1,496,053 + -0.5 1,496,053 + -0.3 1,496,053 + -1.8 1,496,053 + +0.1 1,496,053 + -0.1 1,496,053 + -0.8 1,496,053 + +0.7 1,496,053 + +0.2 1,496,053 + +0.0 1,496,053 + -1.2 1,496,053 + +1.0 1,496,053 + -0.3 1,496,053 + +1.8 1,496,053 + +0.0 1,496,053 + -1.1 1,496,053 + +1.1 1,496,053 + -0.9 1,496,145 + Pf1N1B4_1313 0.12 +0.3 1,496,145 + Pf1N1B4_1313 0.12 +1.0 1,496,145 + Pf1N1B4_1313 0.12 -2.2 1,496,145 + Pf1N1B4_1313 0.12 -0.2 1,496,145 + Pf1N1B4_1313 0.12 -0.5 1,496,320 + Pf1N1B4_1313 0.29 +0.5 1,496,564 + Pf1N1B4_1313 0.53 +0.9 1,496,564 + Pf1N1B4_1313 0.53 +0.3 1,496,564 + Pf1N1B4_1313 0.53 +0.1 1,496,564 + Pf1N1B4_1313 0.53 +1.3 1,496,564 + Pf1N1B4_1313 0.53 +0.4 1,496,564 + Pf1N1B4_1313 0.53 -0.2 1,496,564 + Pf1N1B4_1313 0.53 +0.8 1,496,564 + Pf1N1B4_1313 0.53 -0.2 1,496,582 + Pf1N1B4_1313 0.55 -0.2 1,496,753 + Pf1N1B4_1313 0.72 -0.1 1,496,753 + Pf1N1B4_1313 0.72 +1.0 1,496,753 + Pf1N1B4_1313 0.72 -0.6
Or see this region's nucleotide sequence