Experiment: ALP with Zinc 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2915 and Dsui_2916 are separated by 172 nucleotides Dsui_2916 and Dsui_2917 overlap by 4 nucleotides Dsui_2917 and Dsui_2918 are separated by 32 nucleotides Dsui_2918 and Dsui_2919 are separated by 161 nucleotides
Dsui_2915: Dsui_2915 - o-succinylbenzoic acid synthetase, at 3,117,563 to 3,118,627
_2915
Dsui_2916: Dsui_2916 - hypothetical protein, at 3,118,800 to 3,119,177
_2916
Dsui_2917: Dsui_2917 - hypothetical protein, at 3,119,174 to 3,119,572
_2917
Dsui_2918: Dsui_2918 - hypothetical protein, at 3,119,605 to 3,120,285
_2918
Dsui_2919: Dsui_2919 - addiction module antidote protein, HigA family, at 3,120,447 to 3,120,761
_2919
Position (kb)
3119
3120 Strain fitness (log2 ratio)
-2
-1
0
1 at 3118.222 kb on + strand, within Dsui_2915 at 3119.056 kb on + strand at 3119.192 kb on - strand at 3119.428 kb on - strand at 3119.428 kb on - strand at 3119.595 kb on - strand at 3119.595 kb on - strand at 3119.636 kb on + strand at 3119.700 kb on - strand at 3119.758 kb on - strand at 3119.987 kb on - strand at 3119.991 kb on + strand at 3120.110 kb on - strand at 3120.160 kb on - strand at 3120.367 kb on + strand at 3120.463 kb on + strand at 3120.465 kb on + strand at 3120.478 kb on + strand at 3120.486 kb on - strand at 3120.488 kb on + strand, within Dsui_2919 at 3120.488 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Zinc 2 mM remove 3,118,222 + Dsui_2915 0.62 -2.1 3,119,056 + -0.1 3,119,192 - -0.5 3,119,428 - +0.5 3,119,428 - +0.5 3,119,595 - +0.5 3,119,595 - -1.8 3,119,636 + +0.5 3,119,700 - +0.5 3,119,758 - +0.5 3,119,987 - +0.5 3,119,991 + +0.5 3,120,110 - -0.5 3,120,160 - +0.5 3,120,367 + -0.5 3,120,463 + -2.3 3,120,465 + +0.5 3,120,478 + -0.5 3,120,486 - +0.5 3,120,488 + Dsui_2919 0.13 -0.5 3,120,488 + +0.5
Or see this region's nucleotide sequence