Experiment: ALP with Zinc 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1665 and Dsui_1666 overlap by 16 nucleotides Dsui_1666 and Dsui_1667 are separated by 79 nucleotides Dsui_1667 and Dsui_1668 are separated by 249 nucleotides
Dsui_1665: Dsui_1665 - hypothetical protein, at 1,748,888 to 1,749,547
_1665
Dsui_1666: Dsui_1666 - putative iron-regulated protein, at 1,749,532 to 1,750,212
_1666
Dsui_1667: Dsui_1667 - TonB-dependent siderophore receptor, at 1,750,292 to 1,752,595
_1667
Dsui_1668: Dsui_1668 - alpha-hydroxyacid dehydrogenase, FMN-dependent L-lactate dehydrogenase, at 1,752,845 to 1,753,969
_1668
Position (kb)
1750
1751
1752
1753 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1749.358 kb on - strand, within Dsui_1665 at 1749.513 kb on + strand at 1749.530 kb on + strand at 1749.538 kb on - strand at 1749.538 kb on - strand at 1750.221 kb on + strand at 1750.235 kb on + strand at 1752.554 kb on - strand at 1752.901 kb on + strand at 1752.909 kb on - strand at 1752.929 kb on - strand at 1752.960 kb on - strand, within Dsui_1668 at 1752.960 kb on - strand, within Dsui_1668 at 1753.143 kb on - strand, within Dsui_1668 at 1753.309 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Zinc 2 mM remove 1,749,358 - Dsui_1665 0.71 -0.2 1,749,513 + +0.4 1,749,530 + -1.2 1,749,538 - +0.0 1,749,538 - -0.3 1,750,221 + +0.4 1,750,235 + -1.2 1,752,554 - -3.3 1,752,901 + +0.4 1,752,909 - +0.4 1,752,929 - -1.6 1,752,960 - Dsui_1668 0.10 -0.6 1,752,960 - Dsui_1668 0.10 -1.9 1,753,143 - Dsui_1668 0.26 -1.6 1,753,309 - -0.6
Or see this region's nucleotide sequence