Experiment: ALP with Zinc 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1159 and Dsui_1160 are separated by 79 nucleotides Dsui_1160 and Dsui_1161 are separated by 2 nucleotides Dsui_1161 and Dsui_1162 are separated by 9 nucleotides
Dsui_1159: Dsui_1159 - hydrogenase expression/formation protein HypD, at 1,215,031 to 1,216,161
_1159
Dsui_1160: Dsui_1160 - hydrogenase assembly chaperone HypC/HupF, at 1,216,241 to 1,216,486
_1160
Dsui_1161: Dsui_1161 - transglutaminase-like enzyme, predicted cysteine protease, at 1,216,489 to 1,217,133
_1161
Dsui_1162: Dsui_1162 - (NiFe) hydrogenase maturation protein HypF, at 1,217,143 to 1,219,482
_1162
Position (kb)
1216
1217
1218 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1215.497 kb on - strand, within Dsui_1159 at 1215.550 kb on + strand, within Dsui_1159 at 1215.653 kb on - strand, within Dsui_1159 at 1215.680 kb on + strand at 1215.694 kb on - strand, within Dsui_1159 at 1215.802 kb on + strand at 1215.810 kb on - strand, within Dsui_1159 at 1215.881 kb on - strand, within Dsui_1159 at 1215.900 kb on + strand, within Dsui_1159 at 1215.901 kb on - strand, within Dsui_1159 at 1216.049 kb on - strand at 1216.093 kb on - strand at 1216.240 kb on - strand at 1216.240 kb on - strand at 1216.247 kb on - strand at 1216.268 kb on + strand, within Dsui_1160 at 1216.326 kb on + strand, within Dsui_1160 at 1216.334 kb on - strand, within Dsui_1160 at 1216.337 kb on + strand, within Dsui_1160 at 1216.386 kb on + strand at 1216.390 kb on + strand, within Dsui_1160 at 1216.487 kb on + strand at 1216.495 kb on - strand at 1216.552 kb on + strand at 1216.600 kb on - strand, within Dsui_1161 at 1216.659 kb on - strand, within Dsui_1161 at 1216.659 kb on - strand, within Dsui_1161 at 1216.703 kb on - strand, within Dsui_1161 at 1216.740 kb on - strand, within Dsui_1161 at 1216.786 kb on - strand, within Dsui_1161 at 1216.909 kb on - strand, within Dsui_1161 at 1216.924 kb on + strand at 1217.032 kb on + strand, within Dsui_1161 at 1217.320 kb on - strand at 1217.442 kb on + strand at 1217.459 kb on + strand at 1217.506 kb on - strand, within Dsui_1162 at 1217.516 kb on - strand, within Dsui_1162 at 1217.520 kb on + strand at 1217.529 kb on - strand, within Dsui_1162 at 1217.616 kb on + strand at 1217.624 kb on - strand, within Dsui_1162 at 1217.624 kb on - strand, within Dsui_1162 at 1217.624 kb on - strand, within Dsui_1162 at 1217.633 kb on + strand, within Dsui_1162 at 1217.677 kb on + strand at 1217.677 kb on + strand, within Dsui_1162 at 1217.797 kb on + strand at 1217.968 kb on - strand, within Dsui_1162 at 1218.021 kb on + strand at 1218.048 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Zinc 2 mM remove 1,215,497 - Dsui_1159 0.41 +0.6 1,215,550 + Dsui_1159 0.46 -0.7 1,215,653 - Dsui_1159 0.55 -0.5 1,215,680 + +0.3 1,215,694 - Dsui_1159 0.59 -0.7 1,215,802 + +0.3 1,215,810 - Dsui_1159 0.69 -1.7 1,215,881 - Dsui_1159 0.75 -0.5 1,215,900 + Dsui_1159 0.77 +0.3 1,215,901 - Dsui_1159 0.77 -4.0 1,216,049 - -2.0 1,216,093 - -0.7 1,216,240 - -0.3 1,216,240 - -0.7 1,216,247 - -0.9 1,216,268 + Dsui_1160 0.11 -1.3 1,216,326 + Dsui_1160 0.35 +0.3 1,216,334 - Dsui_1160 0.38 -1.7 1,216,337 + Dsui_1160 0.39 -2.0 1,216,386 + +0.3 1,216,390 + Dsui_1160 0.61 -0.7 1,216,487 + -0.7 1,216,495 - -1.3 1,216,552 + -0.7 1,216,600 - Dsui_1161 0.17 -1.3 1,216,659 - Dsui_1161 0.26 -1.9 1,216,659 - Dsui_1161 0.26 -1.3 1,216,703 - Dsui_1161 0.33 +0.7 1,216,740 - Dsui_1161 0.39 -0.6 1,216,786 - Dsui_1161 0.46 -0.7 1,216,909 - Dsui_1161 0.65 -2.4 1,216,924 + +0.3 1,217,032 + Dsui_1161 0.84 -0.7 1,217,320 - -1.1 1,217,442 + +0.3 1,217,459 + +0.3 1,217,506 - Dsui_1162 0.16 -0.3 1,217,516 - Dsui_1162 0.16 -1.0 1,217,520 + +0.3 1,217,529 - Dsui_1162 0.16 -1.4 1,217,616 + +0.3 1,217,624 - Dsui_1162 0.21 +0.0 1,217,624 - Dsui_1162 0.21 -1.5 1,217,624 - Dsui_1162 0.21 +0.3 1,217,633 + Dsui_1162 0.21 -0.7 1,217,677 + +0.3 1,217,677 + Dsui_1162 0.23 -1.3 1,217,797 + +0.3 1,217,968 - Dsui_1162 0.35 -1.0 1,218,021 + -1.3 1,218,048 + +0.3
Or see this region's nucleotide sequence