Experiment: ALP with Zinc 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1152 and Dsui_1153 overlap by 4 nucleotides Dsui_1153 and Dsui_1154 are separated by 139 nucleotides
Dsui_1152: Dsui_1152 - Ni,Fe-hydrogenase I large subunit, at 1,206,546 to 1,208,006
_1152
Dsui_1153: Dsui_1153 - Ni,Fe-hydrogenase I small subunit, at 1,208,003 to 1,208,998
_1153
Dsui_1154: Dsui_1154 - response regulator with CheY-like receiver, AAA-type ATPase, and DNA-binding domains, at 1,209,138 to 1,210,613
_1154
Position (kb)
1208
1209 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1207.031 kb on - strand, within Dsui_1152 at 1207.064 kb on - strand, within Dsui_1152 at 1207.089 kb on + strand, within Dsui_1152 at 1207.107 kb on + strand at 1207.156 kb on - strand, within Dsui_1152 at 1207.352 kb on + strand, within Dsui_1152 at 1207.447 kb on + strand, within Dsui_1152 at 1207.455 kb on - strand, within Dsui_1152 at 1207.455 kb on - strand, within Dsui_1152 at 1207.497 kb on - strand, within Dsui_1152 at 1207.575 kb on + strand at 1207.724 kb on + strand, within Dsui_1152 at 1207.728 kb on - strand at 1207.781 kb on - strand, within Dsui_1152 at 1207.904 kb on - strand at 1207.906 kb on + strand at 1207.943 kb on + strand at 1207.943 kb on + strand at 1207.943 kb on + strand at 1207.951 kb on - strand at 1207.957 kb on - strand at 1207.966 kb on + strand at 1207.985 kb on - strand at 1208.014 kb on - strand at 1208.046 kb on - strand at 1208.122 kb on - strand, within Dsui_1153 at 1208.133 kb on + strand, within Dsui_1153 at 1208.165 kb on - strand, within Dsui_1153 at 1208.216 kb on + strand, within Dsui_1153 at 1208.269 kb on - strand, within Dsui_1153 at 1208.335 kb on + strand, within Dsui_1153 at 1208.415 kb on - strand, within Dsui_1153 at 1208.562 kb on + strand, within Dsui_1153 at 1208.719 kb on - strand, within Dsui_1153 at 1209.019 kb on + strand at 1209.038 kb on - strand at 1209.066 kb on + strand at 1209.187 kb on - strand at 1209.195 kb on + strand at 1209.211 kb on + strand at 1209.219 kb on - strand at 1209.284 kb on + strand at 1209.303 kb on - strand, within Dsui_1154 at 1209.357 kb on - strand, within Dsui_1154 at 1209.394 kb on + strand, within Dsui_1154 at 1209.427 kb on - strand, within Dsui_1154 at 1209.505 kb on + strand, within Dsui_1154 at 1209.505 kb on + strand, within Dsui_1154 at 1209.596 kb on + strand, within Dsui_1154 at 1209.596 kb on + strand, within Dsui_1154 at 1209.604 kb on - strand, within Dsui_1154 at 1209.717 kb on + strand at 1209.724 kb on + strand, within Dsui_1154 at 1209.814 kb on + strand, within Dsui_1154
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Zinc 2 mM remove 1,207,031 - Dsui_1152 0.33 -0.9 1,207,064 - Dsui_1152 0.35 +1.5 1,207,089 + Dsui_1152 0.37 -0.7 1,207,107 + +0.3 1,207,156 - Dsui_1152 0.42 +0.3 1,207,352 + Dsui_1152 0.55 -1.3 1,207,447 + Dsui_1152 0.62 -2.9 1,207,455 - Dsui_1152 0.62 -2.5 1,207,455 - Dsui_1152 0.62 -2.5 1,207,497 - Dsui_1152 0.65 -0.5 1,207,575 + -0.7 1,207,724 + Dsui_1152 0.81 +0.3 1,207,728 - -0.7 1,207,781 - Dsui_1152 0.85 -0.7 1,207,904 - +0.3 1,207,906 + +0.3 1,207,943 + +0.3 1,207,943 + -0.7 1,207,943 + +0.3 1,207,951 - -0.8 1,207,957 - -1.9 1,207,966 + -2.5 1,207,985 - -2.2 1,208,014 - -1.2 1,208,046 - -1.5 1,208,122 - Dsui_1153 0.12 -2.5 1,208,133 + Dsui_1153 0.13 -1.3 1,208,165 - Dsui_1153 0.16 -0.8 1,208,216 + Dsui_1153 0.21 -0.7 1,208,269 - Dsui_1153 0.27 -0.8 1,208,335 + Dsui_1153 0.33 -0.7 1,208,415 - Dsui_1153 0.41 -0.5 1,208,562 + Dsui_1153 0.56 -0.7 1,208,719 - Dsui_1153 0.72 +0.2 1,209,019 + +0.3 1,209,038 - -3.0 1,209,066 + +0.3 1,209,187 - +0.5 1,209,195 + +0.3 1,209,211 + +0.3 1,209,219 - +1.3 1,209,284 + +0.3 1,209,303 - Dsui_1154 0.11 -1.1 1,209,357 - Dsui_1154 0.15 -0.3 1,209,394 + Dsui_1154 0.17 -1.3 1,209,427 - Dsui_1154 0.20 -0.7 1,209,505 + Dsui_1154 0.25 +0.3 1,209,505 + Dsui_1154 0.25 -1.7 1,209,596 + Dsui_1154 0.31 +0.3 1,209,596 + Dsui_1154 0.31 -1.3 1,209,604 - Dsui_1154 0.32 -0.8 1,209,717 + -0.7 1,209,724 + Dsui_1154 0.40 -1.7 1,209,814 + Dsui_1154 0.46 +0.3
Or see this region's nucleotide sequence