Experiment: ALP with Zinc 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0482 and Dsui_0483 overlap by 4 nucleotides Dsui_0483 and Dsui_0484 are separated by 58 nucleotides Dsui_0484 and Dsui_0485 are separated by 74 nucleotides Dsui_0485 and Dsui_0486 overlap by 4 nucleotides
Dsui_0482: Dsui_0482 - TolQ protein, at 486,429 to 487,106
_0482
Dsui_0483: Dsui_0483 - tol-pal system-associated acyl-CoA thioesterase, at 487,103 to 487,528
_0483
Dsui_0484: Dsui_0484 - Holliday junction DNA helicase, RuvB subunit, at 487,587 to 488,669
_0484
Dsui_0485: Dsui_0485 - Holliday junction DNA helicase, RuvA subunit, at 488,744 to 489,328
_0485
Dsui_0486: Dsui_0486 - crossover junction endodeoxyribonuclease RuvC, at 489,325 to 489,930
_0486
Position (kb) 487
488
489 Strain fitness (log2 ratio) -2
-1
0
1 at 487.176 kb on + strand, within Dsui_0483 at 487.199 kb on - strand, within Dsui_0483 at 487.250 kb on - strand, within Dsui_0483 at 487.284 kb on + strand, within Dsui_0483 at 487.294 kb on - strand, within Dsui_0483 at 487.490 kb on + strand at 487.513 kb on - strand at 487.539 kb on - strand at 487.585 kb on + strand at 487.618 kb on - strand at 489.354 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Zinc 2 mM remove 487,176 + Dsui_0483 0.17 -1.8 487,199 - Dsui_0483 0.23 -0.4 487,250 - Dsui_0483 0.35 -0.5 487,284 + Dsui_0483 0.42 -0.7 487,294 - Dsui_0483 0.45 -0.2 487,490 + +0.3 487,513 - -0.2 487,539 - -0.4 487,585 + -1.7 487,618 - -0.2 489,354 - -0.5
Or see this region's nucleotide sequence