Experiment: Recovery broth with nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13940 and BBR_RS13945 are separated by 168 nucleotides BBR_RS13945 and BBR_RS13950 are separated by 1 nucleotides BBR_RS13950 and BBR_RS20810 are separated by 108 nucleotides BBR_RS20810 and BBR_RS13955 are separated by 9 nucleotides BBR_RS13955 and BBR_RS13960 are separated by 439 nucleotides BBR_RS13960 and BBR_RS13965 are separated by 11 nucleotides
BBR_RS13940: BBR_RS13940 - integrase, at 906,789 to 907,040
_RS13940
BBR_RS13945: BBR_RS13945 - Txe/YoeB family addiction module toxin, at 907,209 to 907,484
_RS13945
BBR_RS13950: BBR_RS13950 - type II toxin-antitoxin system antitoxin, RelB/DinJ family, at 907,486 to 907,740
_RS13950
BBR_RS20810: BBR_RS20810 - pirin, at 907,849 to 907,959
_RS20810
BBR_RS13955: BBR_RS13955 - argininosuccinate lyase, at 907,969 to 909,441
_RS13955
BBR_RS13960: BBR_RS13960 - thiamine biosynthesis protein ThiS, at 909,881 to 910,075
_RS13960
BBR_RS13965: BBR_RS13965 - thiazole synthase, at 910,087 to 910,956
_RS13965
Position (kb) 907
908
909
910 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 906.989 kb on + strand, within BBR_RS13940 at 907.005 kb on + strand, within BBR_RS13940 at 907.072 kb on - strand at 907.078 kb on - strand at 907.078 kb on - strand at 907.153 kb on - strand at 907.155 kb on - strand at 907.158 kb on - strand at 907.158 kb on - strand at 907.174 kb on - strand at 907.183 kb on + strand at 907.184 kb on - strand at 907.207 kb on + strand at 907.207 kb on + strand at 907.207 kb on + strand at 907.207 kb on + strand at 907.214 kb on + strand at 907.215 kb on - strand at 907.215 kb on - strand at 907.234 kb on - strand at 907.307 kb on + strand, within BBR_RS13945 at 907.350 kb on - strand, within BBR_RS13945 at 907.360 kb on - strand, within BBR_RS13945 at 907.434 kb on + strand, within BBR_RS13945 at 907.479 kb on + strand at 907.904 kb on + strand, within BBR_RS20810 at 907.920 kb on + strand, within BBR_RS20810 at 907.920 kb on + strand, within BBR_RS20810 at 907.953 kb on + strand at 907.953 kb on + strand at 907.961 kb on - strand at 907.977 kb on + strand at 907.995 kb on - strand at 908.037 kb on + strand at 908.046 kb on + strand at 908.046 kb on + strand at 908.054 kb on - strand at 908.054 kb on - strand at 908.054 kb on - strand at 908.109 kb on + strand at 908.116 kb on + strand at 908.116 kb on + strand at 908.141 kb on + strand, within BBR_RS13955 at 908.144 kb on - strand, within BBR_RS13955 at 908.152 kb on + strand, within BBR_RS13955 at 908.165 kb on - strand, within BBR_RS13955 at 908.182 kb on + strand, within BBR_RS13955 at 908.182 kb on + strand, within BBR_RS13955 at 908.182 kb on + strand, within BBR_RS13955 at 908.190 kb on - strand, within BBR_RS13955 at 908.207 kb on + strand, within BBR_RS13955 at 908.247 kb on + strand, within BBR_RS13955 at 908.281 kb on + strand, within BBR_RS13955 at 908.401 kb on + strand, within BBR_RS13955 at 908.402 kb on + strand, within BBR_RS13955 at 908.457 kb on + strand, within BBR_RS13955 at 908.496 kb on - strand, within BBR_RS13955 at 908.561 kb on + strand, within BBR_RS13955 at 908.569 kb on + strand, within BBR_RS13955 at 908.584 kb on - strand, within BBR_RS13955 at 908.592 kb on + strand, within BBR_RS13955 at 908.606 kb on + strand, within BBR_RS13955 at 908.681 kb on + strand, within BBR_RS13955 at 908.681 kb on + strand, within BBR_RS13955 at 908.735 kb on + strand, within BBR_RS13955 at 908.778 kb on - strand, within BBR_RS13955 at 908.784 kb on - strand, within BBR_RS13955 at 908.916 kb on + strand, within BBR_RS13955 at 908.916 kb on + strand, within BBR_RS13955 at 908.922 kb on + strand, within BBR_RS13955 at 908.922 kb on + strand, within BBR_RS13955 at 908.923 kb on + strand, within BBR_RS13955 at 908.923 kb on + strand, within BBR_RS13955 at 908.929 kb on + strand, within BBR_RS13955 at 908.971 kb on - strand, within BBR_RS13955 at 908.999 kb on - strand, within BBR_RS13955 at 909.024 kb on + strand, within BBR_RS13955 at 909.024 kb on + strand, within BBR_RS13955 at 909.024 kb on + strand, within BBR_RS13955 at 909.032 kb on - strand, within BBR_RS13955 at 909.032 kb on - strand, within BBR_RS13955 at 909.032 kb on - strand, within BBR_RS13955 at 909.032 kb on - strand, within BBR_RS13955 at 909.032 kb on - strand, within BBR_RS13955 at 909.096 kb on - strand, within BBR_RS13955 at 909.120 kb on - strand, within BBR_RS13955 at 909.139 kb on + strand, within BBR_RS13955 at 909.158 kb on - strand, within BBR_RS13955 at 909.161 kb on + strand, within BBR_RS13955 at 909.179 kb on + strand, within BBR_RS13955 at 909.179 kb on + strand, within BBR_RS13955 at 909.187 kb on + strand, within BBR_RS13955 at 909.251 kb on + strand, within BBR_RS13955 at 909.274 kb on - strand, within BBR_RS13955 at 909.325 kb on + strand at 909.325 kb on + strand at 909.333 kb on - strand at 909.333 kb on - strand at 909.401 kb on + strand at 909.415 kb on + strand at 909.435 kb on + strand at 909.435 kb on + strand at 909.499 kb on + strand at 909.519 kb on + strand at 909.519 kb on + strand at 909.533 kb on - strand at 909.593 kb on - strand at 909.593 kb on - strand at 909.593 kb on - strand at 909.593 kb on - strand at 909.769 kb on + strand at 909.827 kb on + strand at 909.863 kb on - strand at 909.886 kb on + strand at 909.886 kb on + strand at 909.894 kb on - strand at 909.894 kb on - strand at 909.924 kb on + strand, within BBR_RS13960 at 909.932 kb on - strand, within BBR_RS13960 at 910.056 kb on + strand at 910.094 kb on + strand at 910.167 kb on + strand at 910.167 kb on + strand at 910.190 kb on - strand, within BBR_RS13965 at 910.192 kb on + strand, within BBR_RS13965 at 910.264 kb on + strand, within BBR_RS13965 at 910.272 kb on - strand, within BBR_RS13965 at 910.272 kb on - strand, within BBR_RS13965 at 910.275 kb on + strand, within BBR_RS13965 at 910.341 kb on - strand, within BBR_RS13965 at 910.342 kb on + strand, within BBR_RS13965 at 910.342 kb on + strand, within BBR_RS13965 at 910.350 kb on - strand, within BBR_RS13965 at 910.350 kb on - strand, within BBR_RS13965 at 910.350 kb on - strand, within BBR_RS13965 at 910.350 kb on - strand, within BBR_RS13965 at 910.395 kb on - strand, within BBR_RS13965 at 910.397 kb on - strand, within BBR_RS13965 at 910.405 kb on - strand, within BBR_RS13965 at 910.405 kb on - strand, within BBR_RS13965 at 910.420 kb on - strand, within BBR_RS13965 at 910.420 kb on - strand, within BBR_RS13965 at 910.441 kb on - strand, within BBR_RS13965
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with nitrate 200 mM remove 906,989 + BBR_RS13940 0.79 +0.7 907,005 + BBR_RS13940 0.86 +0.2 907,072 - -0.3 907,078 - -0.5 907,078 - -0.0 907,153 - -0.8 907,155 - -1.0 907,158 - -0.3 907,158 - +0.0 907,174 - -0.2 907,183 + -0.7 907,184 - +0.1 907,207 + -0.7 907,207 + -0.4 907,207 + -0.6 907,207 + +0.4 907,214 + -0.7 907,215 - +0.6 907,215 - -0.7 907,234 - +0.2 907,307 + BBR_RS13945 0.36 +0.2 907,350 - BBR_RS13945 0.51 +0.3 907,360 - BBR_RS13945 0.55 -0.6 907,434 + BBR_RS13945 0.82 +0.5 907,479 + -0.7 907,904 + BBR_RS20810 0.50 -2.4 907,920 + BBR_RS20810 0.64 +0.7 907,920 + BBR_RS20810 0.64 +0.6 907,953 + +1.3 907,953 + -0.4 907,961 - +0.3 907,977 + -1.9 907,995 - +0.5 908,037 + +0.3 908,046 + +0.1 908,046 + -0.4 908,054 - +0.4 908,054 - +0.7 908,054 - -0.7 908,109 + +0.6 908,116 + -0.6 908,116 + +0.3 908,141 + BBR_RS13955 0.12 +0.5 908,144 - BBR_RS13955 0.12 -0.2 908,152 + BBR_RS13955 0.12 -0.4 908,165 - BBR_RS13955 0.13 -1.4 908,182 + BBR_RS13955 0.14 -1.3 908,182 + BBR_RS13955 0.14 +0.1 908,182 + BBR_RS13955 0.14 -0.8 908,190 - BBR_RS13955 0.15 -0.1 908,207 + BBR_RS13955 0.16 -0.6 908,247 + BBR_RS13955 0.19 +0.3 908,281 + BBR_RS13955 0.21 -0.9 908,401 + BBR_RS13955 0.29 -1.0 908,402 + BBR_RS13955 0.29 +1.2 908,457 + BBR_RS13955 0.33 +1.0 908,496 - BBR_RS13955 0.36 +0.6 908,561 + BBR_RS13955 0.40 -0.1 908,569 + BBR_RS13955 0.41 +1.7 908,584 - BBR_RS13955 0.42 +0.6 908,592 + BBR_RS13955 0.42 +0.5 908,606 + BBR_RS13955 0.43 +0.0 908,681 + BBR_RS13955 0.48 -1.6 908,681 + BBR_RS13955 0.48 +0.9 908,735 + BBR_RS13955 0.52 -1.4 908,778 - BBR_RS13955 0.55 +0.7 908,784 - BBR_RS13955 0.55 +1.8 908,916 + BBR_RS13955 0.64 -0.9 908,916 + BBR_RS13955 0.64 -1.2 908,922 + BBR_RS13955 0.65 +0.5 908,922 + BBR_RS13955 0.65 +0.0 908,923 + BBR_RS13955 0.65 -0.9 908,923 + BBR_RS13955 0.65 +1.3 908,929 + BBR_RS13955 0.65 -0.7 908,971 - BBR_RS13955 0.68 +0.7 908,999 - BBR_RS13955 0.70 +0.1 909,024 + BBR_RS13955 0.72 -0.2 909,024 + BBR_RS13955 0.72 -0.3 909,024 + BBR_RS13955 0.72 +0.4 909,032 - BBR_RS13955 0.72 -0.6 909,032 - BBR_RS13955 0.72 -1.6 909,032 - BBR_RS13955 0.72 -0.6 909,032 - BBR_RS13955 0.72 -0.5 909,032 - BBR_RS13955 0.72 -0.7 909,096 - BBR_RS13955 0.77 -0.5 909,120 - BBR_RS13955 0.78 -1.7 909,139 + BBR_RS13955 0.79 -0.8 909,158 - BBR_RS13955 0.81 +0.3 909,161 + BBR_RS13955 0.81 +0.4 909,179 + BBR_RS13955 0.82 +0.8 909,179 + BBR_RS13955 0.82 -0.2 909,187 + BBR_RS13955 0.83 -1.5 909,251 + BBR_RS13955 0.87 -0.7 909,274 - BBR_RS13955 0.89 +0.8 909,325 + +0.2 909,325 + -0.3 909,333 - -0.2 909,333 - -1.0 909,401 + -0.3 909,415 + -0.5 909,435 + +0.3 909,435 + -0.5 909,499 + -0.7 909,519 + +0.2 909,519 + -0.8 909,533 - +2.7 909,593 - -0.7 909,593 - -0.7 909,593 - +0.9 909,593 - -0.8 909,769 + -0.3 909,827 + -1.3 909,863 - +0.2 909,886 + +0.1 909,886 + +0.7 909,894 - -0.6 909,894 - -0.5 909,924 + BBR_RS13960 0.22 -0.7 909,932 - BBR_RS13960 0.26 -0.5 910,056 + -0.1 910,094 + -0.4 910,167 + -0.3 910,167 + -0.7 910,190 - BBR_RS13965 0.12 -1.4 910,192 + BBR_RS13965 0.12 -1.1 910,264 + BBR_RS13965 0.20 +0.3 910,272 - BBR_RS13965 0.21 -0.6 910,272 - BBR_RS13965 0.21 -1.9 910,275 + BBR_RS13965 0.22 +0.3 910,341 - BBR_RS13965 0.29 +0.3 910,342 + BBR_RS13965 0.29 -0.5 910,342 + BBR_RS13965 0.29 -0.5 910,350 - BBR_RS13965 0.30 -0.1 910,350 - BBR_RS13965 0.30 +1.0 910,350 - BBR_RS13965 0.30 +2.9 910,350 - BBR_RS13965 0.30 -0.2 910,395 - BBR_RS13965 0.35 +1.7 910,397 - BBR_RS13965 0.36 +1.3 910,405 - BBR_RS13965 0.37 +0.2 910,405 - BBR_RS13965 0.37 +1.5 910,420 - BBR_RS13965 0.38 +0.5 910,420 - BBR_RS13965 0.38 -0.3 910,441 - BBR_RS13965 0.41 +0.7
Or see this region's nucleotide sequence