Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_141

Experiment: LB 20C

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_140 and Pf1N1B4_141 are separated by 272 nucleotidesPf1N1B4_141 and Pf1N1B4_142 are separated by 210 nucleotides Pf1N1B4_140 - PvdO, pyoverdine responsive serine/threonine kinase (predicted by OlgaV), at 157,242 to 158,129 _140 Pf1N1B4_141 - PvdE, pyoverdine ABC export system, fused ATPase and permease components, at 158,402 to 160,051 _141 Pf1N1B4_142 - TonB-dependent siderophore receptor precursor, at 160,262 to 162,742 _142 Position (kb) 158 159 160 161Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 157.542 kb on + strand, within Pf1N1B4_140at 157.677 kb on + strand, within Pf1N1B4_140at 157.677 kb on + strand, within Pf1N1B4_140at 157.677 kb on + strand, within Pf1N1B4_140at 157.677 kb on + strand, within Pf1N1B4_140at 157.743 kb on + strand, within Pf1N1B4_140at 157.744 kb on - strandat 157.831 kb on - strand, within Pf1N1B4_140at 157.848 kb on + strand, within Pf1N1B4_140at 157.921 kb on - strand, within Pf1N1B4_140at 157.921 kb on - strand, within Pf1N1B4_140at 157.953 kb on + strand, within Pf1N1B4_140at 157.953 kb on + strand, within Pf1N1B4_140at 157.954 kb on - strand, within Pf1N1B4_140at 157.954 kb on - strand, within Pf1N1B4_140at 157.954 kb on - strand, within Pf1N1B4_140at 157.954 kb on - strand, within Pf1N1B4_140at 158.113 kb on + strandat 158.215 kb on + strandat 158.230 kb on - strandat 158.230 kb on - strandat 158.314 kb on + strandat 158.315 kb on - strandat 158.335 kb on + strandat 158.336 kb on - strandat 158.365 kb on + strandat 158.645 kb on + strand, within Pf1N1B4_141at 158.645 kb on + strand, within Pf1N1B4_141at 158.645 kb on + strand, within Pf1N1B4_141at 158.645 kb on + strand, within Pf1N1B4_141at 158.646 kb on - strand, within Pf1N1B4_141at 158.769 kb on + strand, within Pf1N1B4_141at 158.770 kb on - strand, within Pf1N1B4_141at 158.770 kb on - strand, within Pf1N1B4_141at 158.878 kb on - strand, within Pf1N1B4_141at 158.878 kb on - strand, within Pf1N1B4_141at 159.116 kb on - strand, within Pf1N1B4_141at 159.116 kb on - strand, within Pf1N1B4_141at 159.245 kb on + strand, within Pf1N1B4_141at 159.245 kb on + strand, within Pf1N1B4_141at 159.245 kb on + strand, within Pf1N1B4_141at 159.246 kb on - strand, within Pf1N1B4_141at 159.384 kb on - strand, within Pf1N1B4_141at 159.569 kb on + strand, within Pf1N1B4_141at 159.570 kb on - strand, within Pf1N1B4_141at 159.668 kb on + strand, within Pf1N1B4_141at 159.669 kb on - strand, within Pf1N1B4_141at 159.669 kb on - strand, within Pf1N1B4_141at 159.669 kb on - strand, within Pf1N1B4_141at 159.671 kb on + strand, within Pf1N1B4_141at 159.672 kb on - strand, within Pf1N1B4_141at 159.729 kb on - strand, within Pf1N1B4_141at 160.068 kb on - strandat 160.145 kb on - strandat 160.145 kb on - strandat 160.145 kb on - strandat 160.145 kb on - strandat 160.239 kb on - strandat 160.382 kb on + strandat 160.382 kb on + strandat 160.382 kb on + strandat 160.382 kb on + strandat 160.478 kb on + strandat 160.478 kb on + strandat 160.479 kb on - strandat 160.479 kb on - strandat 160.479 kb on - strandat 160.479 kb on - strandat 160.851 kb on - strand, within Pf1N1B4_142at 160.874 kb on + strand, within Pf1N1B4_142at 160.874 kb on + strand, within Pf1N1B4_142at 160.949 kb on + strand, within Pf1N1B4_142at 160.949 kb on + strand, within Pf1N1B4_142at 160.950 kb on - strand, within Pf1N1B4_142at 160.989 kb on - strand, within Pf1N1B4_142at 160.989 kb on - strand, within Pf1N1B4_142at 160.989 kb on - strand, within Pf1N1B4_142

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Per-strain Table

Position Strand Gene LocusTag Fraction LB 20C
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157,542 + Pf1N1B4_140 0.34 -1.4
157,677 + Pf1N1B4_140 0.49 +1.0
157,677 + Pf1N1B4_140 0.49 +0.4
157,677 + Pf1N1B4_140 0.49 -0.5
157,677 + Pf1N1B4_140 0.49 +0.2
157,743 + Pf1N1B4_140 0.56 +0.2
157,744 - +1.8
157,831 - Pf1N1B4_140 0.66 -1.2
157,848 + Pf1N1B4_140 0.68 -1.1
157,921 - Pf1N1B4_140 0.76 -2.2
157,921 - Pf1N1B4_140 0.76 -0.0
157,953 + Pf1N1B4_140 0.80 +0.3
157,953 + Pf1N1B4_140 0.80 -1.7
157,954 - Pf1N1B4_140 0.80 +0.6
157,954 - Pf1N1B4_140 0.80 -1.6
157,954 - Pf1N1B4_140 0.80 +0.4
157,954 - Pf1N1B4_140 0.80 -1.6
158,113 + +0.3
158,215 + -0.8
158,230 - +1.1
158,230 - -0.4
158,314 + -0.8
158,315 - -0.5
158,335 + -0.8
158,336 - -0.5
158,365 + -0.2
158,645 + Pf1N1B4_141 0.15 -0.1
158,645 + Pf1N1B4_141 0.15 -2.5
158,645 + Pf1N1B4_141 0.15 -2.7
158,645 + Pf1N1B4_141 0.15 -2.0
158,646 - Pf1N1B4_141 0.15 -0.0
158,769 + Pf1N1B4_141 0.22 -0.9
158,770 - Pf1N1B4_141 0.22 -0.7
158,770 - Pf1N1B4_141 0.22 -1.6
158,878 - Pf1N1B4_141 0.29 -1.1
158,878 - Pf1N1B4_141 0.29 -1.4
159,116 - Pf1N1B4_141 0.43 -0.9
159,116 - Pf1N1B4_141 0.43 -0.5
159,245 + Pf1N1B4_141 0.51 -2.8
159,245 + Pf1N1B4_141 0.51 -0.5
159,245 + Pf1N1B4_141 0.51 -0.2
159,246 - Pf1N1B4_141 0.51 -1.2
159,384 - Pf1N1B4_141 0.60 -0.7
159,569 + Pf1N1B4_141 0.71 -0.6
159,570 - Pf1N1B4_141 0.71 -1.4
159,668 + Pf1N1B4_141 0.77 -1.6
159,669 - Pf1N1B4_141 0.77 -1.4
159,669 - Pf1N1B4_141 0.77 +0.1
159,669 - Pf1N1B4_141 0.77 -1.3
159,671 + Pf1N1B4_141 0.77 -1.5
159,672 - Pf1N1B4_141 0.77 -1.5
159,729 - Pf1N1B4_141 0.80 -1.1
160,068 - -0.9
160,145 - -2.6
160,145 - +0.8
160,145 - +1.1
160,145 - -0.6
160,239 - -0.2
160,382 + -1.4
160,382 + +0.5
160,382 + +0.1
160,382 + -0.2
160,478 + +0.2
160,478 + -0.7
160,479 - -1.5
160,479 - +0.3
160,479 - +0.2
160,479 - +0.5
160,851 - Pf1N1B4_142 0.24 -0.0
160,874 + Pf1N1B4_142 0.25 -1.3
160,874 + Pf1N1B4_142 0.25 -0.2
160,949 + Pf1N1B4_142 0.28 -0.3
160,949 + Pf1N1B4_142 0.28 +0.7
160,950 - Pf1N1B4_142 0.28 -0.5
160,989 - Pf1N1B4_142 0.29 -0.4
160,989 - Pf1N1B4_142 0.29 -0.5
160,989 - Pf1N1B4_142 0.29 +0.4

Or see this region's nucleotide sequence