Experiment: LB 20C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1397 and Pf1N1B4_1398 are separated by 123 nucleotides Pf1N1B4_1398 and Pf1N1B4_1399 are separated by 19 nucleotides Pf1N1B4_1399 and Pf1N1B4_1400 are separated by 36 nucleotides Pf1N1B4_1400 and Pf1N1B4_1401 are separated by 28 nucleotides
Pf1N1B4_1397 - Replicative DNA helicase (EC 3.6.1.-), at 1,601,677 to 1,603,071
_1397
Pf1N1B4_1398 - LSU ribosomal protein L9p, at 1,603,195 to 1,603,641
_1398
Pf1N1B4_1399 - FIG003573: hypothetical protein, at 1,603,661 to 1,604,554
_1399
Pf1N1B4_1400 - SSU ribosomal protein S18p @ SSU ribosomal protein S18p, zinc-independent, at 1,604,591 to 1,604,821
_1400
Pf1N1B4_1401 - SSU ribosomal protein S6p, at 1,604,850 to 1,605,272
_1401
Position (kb)
1603
1604
1605 Strain fitness (log2 ratio)
-2
-1
0
1 at 1603.093 kb on - strand at 1603.205 kb on - strand at 1603.759 kb on - strand at 1603.792 kb on - strand, within Pf1N1B4_1399 at 1603.792 kb on - strand, within Pf1N1B4_1399 at 1603.792 kb on - strand, within Pf1N1B4_1399 at 1603.792 kb on - strand, within Pf1N1B4_1399 at 1603.792 kb on - strand, within Pf1N1B4_1399 at 1603.792 kb on - strand, within Pf1N1B4_1399 at 1603.792 kb on - strand, within Pf1N1B4_1399 at 1603.792 kb on - strand, within Pf1N1B4_1399 at 1603.875 kb on - strand, within Pf1N1B4_1399 at 1603.914 kb on - strand, within Pf1N1B4_1399 at 1603.914 kb on - strand, within Pf1N1B4_1399 at 1603.914 kb on - strand, within Pf1N1B4_1399 at 1603.919 kb on - strand, within Pf1N1B4_1399 at 1603.959 kb on - strand, within Pf1N1B4_1399 at 1604.011 kb on - strand, within Pf1N1B4_1399 at 1604.149 kb on - strand, within Pf1N1B4_1399 at 1604.362 kb on - strand, within Pf1N1B4_1399 at 1605.433 kb on + strand at 1605.434 kb on - strand at 1605.434 kb on - strand at 1605.463 kb on + strand at 1605.463 kb on + strand at 1605.463 kb on + strand at 1605.463 kb on + strand at 1605.463 kb on + strand at 1605.464 kb on - strand at 1605.464 kb on - strand at 1605.464 kb on - strand at 1605.464 kb on - strand at 1605.464 kb on - strand at 1605.535 kb on + strand at 1605.535 kb on + strand at 1605.536 kb on - strand at 1605.536 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 20C remove 1,603,093 - -1.1 1,603,205 - -1.5 1,603,759 - +0.3 1,603,792 - Pf1N1B4_1399 0.15 +0.5 1,603,792 - Pf1N1B4_1399 0.15 -1.4 1,603,792 - Pf1N1B4_1399 0.15 -1.7 1,603,792 - Pf1N1B4_1399 0.15 +0.9 1,603,792 - Pf1N1B4_1399 0.15 +0.3 1,603,792 - Pf1N1B4_1399 0.15 -1.4 1,603,792 - Pf1N1B4_1399 0.15 -2.0 1,603,792 - Pf1N1B4_1399 0.15 -0.1 1,603,875 - Pf1N1B4_1399 0.24 -1.9 1,603,914 - Pf1N1B4_1399 0.28 -1.3 1,603,914 - Pf1N1B4_1399 0.28 -1.0 1,603,914 - Pf1N1B4_1399 0.28 -2.0 1,603,919 - Pf1N1B4_1399 0.29 -0.1 1,603,959 - Pf1N1B4_1399 0.33 -1.5 1,604,011 - Pf1N1B4_1399 0.39 -0.2 1,604,149 - Pf1N1B4_1399 0.55 -1.0 1,604,362 - Pf1N1B4_1399 0.78 -0.7 1,605,433 + -1.0 1,605,434 - +0.9 1,605,434 - -0.6 1,605,463 + -0.2 1,605,463 + -0.6 1,605,463 + +0.4 1,605,463 + -0.3 1,605,463 + -0.3 1,605,464 - +1.2 1,605,464 - -1.8 1,605,464 - -1.7 1,605,464 - -0.1 1,605,464 - -0.2 1,605,535 + +0.5 1,605,535 + -0.1 1,605,536 - -0.2 1,605,536 - -0.7
Or see this region's nucleotide sequence