Experiment: Formate McCas no stress control 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS04470 and MMP_RS04475 overlap by 35 nucleotides MMP_RS04475 and MMP_RS04480 are separated by 575 nucleotides
MMP_RS04470: MMP_RS04470 - nitrogenase, at 849,999 to 851,375
_RS04470
MMP_RS04475: MMP_RS04475 - nitrogen fixation, at 851,341 to 851,661
_RS04475
MMP_RS04480: MMP_RS04480 - lysine 2 3-aminomutase, at 852,237 to 853,538
_RS04480
Position (kb)
851
852 Strain fitness (log2 ratio)
-1
0
1 at 850.360 kb on - strand, within MMP_RS04470 at 850.394 kb on - strand, within MMP_RS04470 at 850.395 kb on + strand, within MMP_RS04470 at 850.395 kb on + strand, within MMP_RS04470 at 850.395 kb on + strand, within MMP_RS04470 at 850.400 kb on - strand, within MMP_RS04470 at 850.401 kb on + strand, within MMP_RS04470 at 850.408 kb on - strand, within MMP_RS04470 at 850.408 kb on - strand, within MMP_RS04470 at 850.408 kb on - strand, within MMP_RS04470 at 850.409 kb on + strand, within MMP_RS04470 at 850.409 kb on + strand, within MMP_RS04470 at 850.480 kb on - strand, within MMP_RS04470 at 850.481 kb on + strand, within MMP_RS04470 at 850.553 kb on + strand, within MMP_RS04470 at 850.562 kb on + strand, within MMP_RS04470 at 850.586 kb on - strand, within MMP_RS04470 at 850.587 kb on + strand, within MMP_RS04470 at 850.587 kb on + strand, within MMP_RS04470 at 850.637 kb on - strand, within MMP_RS04470 at 850.805 kb on - strand, within MMP_RS04470 at 850.838 kb on + strand, within MMP_RS04470 at 850.841 kb on - strand, within MMP_RS04470 at 850.841 kb on - strand, within MMP_RS04470 at 850.934 kb on + strand, within MMP_RS04470 at 851.032 kb on - strand, within MMP_RS04470 at 851.037 kb on + strand, within MMP_RS04470 at 851.052 kb on + strand, within MMP_RS04470 at 851.066 kb on + strand, within MMP_RS04470 at 851.126 kb on + strand, within MMP_RS04470 at 851.180 kb on - strand, within MMP_RS04470 at 851.199 kb on - strand, within MMP_RS04470 at 851.205 kb on + strand, within MMP_RS04470 at 851.239 kb on + strand at 851.240 kb on - strand at 851.241 kb on + strand at 851.260 kb on - strand at 851.261 kb on + strand at 851.264 kb on - strand at 851.264 kb on - strand at 851.264 kb on - strand at 851.267 kb on - strand at 851.268 kb on + strand at 851.268 kb on + strand at 851.269 kb on - strand at 851.269 kb on - strand at 851.276 kb on - strand at 851.293 kb on + strand at 851.382 kb on + strand, within MMP_RS04475 at 851.382 kb on + strand, within MMP_RS04475 at 851.444 kb on - strand, within MMP_RS04475 at 851.484 kb on - strand, within MMP_RS04475 at 851.486 kb on - strand, within MMP_RS04475 at 851.513 kb on + strand, within MMP_RS04475 at 851.934 kb on - strand at 851.944 kb on - strand at 851.945 kb on + strand at 851.957 kb on + strand at 851.966 kb on - strand at 851.971 kb on - strand at 851.972 kb on + strand at 851.975 kb on - strand at 851.976 kb on + strand at 851.976 kb on + strand at 851.976 kb on + strand at 851.998 kb on - strand at 852.002 kb on - strand at 852.041 kb on - strand at 852.046 kb on - strand at 852.051 kb on - strand at 852.051 kb on - strand at 852.051 kb on - strand at 852.051 kb on - strand at 852.054 kb on + strand at 852.056 kb on - strand at 852.057 kb on + strand at 852.057 kb on + strand at 852.074 kb on + strand at 852.102 kb on - strand at 852.102 kb on - strand at 852.103 kb on + strand at 852.105 kb on + strand at 852.179 kb on - strand at 852.180 kb on + strand at 852.247 kb on - strand at 852.248 kb on + strand at 852.260 kb on - strand at 852.265 kb on - strand at 852.319 kb on + strand at 852.421 kb on - strand, within MMP_RS04480 at 852.422 kb on + strand, within MMP_RS04480 at 852.428 kb on + strand, within MMP_RS04480 at 852.428 kb on + strand, within MMP_RS04480 at 852.443 kb on - strand, within MMP_RS04480 at 852.451 kb on + strand, within MMP_RS04480 at 852.453 kb on - strand, within MMP_RS04480 at 852.454 kb on + strand, within MMP_RS04480 at 852.454 kb on + strand, within MMP_RS04480 at 852.454 kb on + strand, within MMP_RS04480 at 852.463 kb on - strand, within MMP_RS04480 at 852.500 kb on - strand, within MMP_RS04480 at 852.533 kb on + strand, within MMP_RS04480 at 852.533 kb on + strand, within MMP_RS04480 at 852.622 kb on - strand, within MMP_RS04480 at 852.627 kb on - strand, within MMP_RS04480 at 852.632 kb on + strand, within MMP_RS04480
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 1 remove 850,360 - MMP_RS04470 0.26 -0.1 850,394 - MMP_RS04470 0.29 +0.5 850,395 + MMP_RS04470 0.29 -0.4 850,395 + MMP_RS04470 0.29 +0.1 850,395 + MMP_RS04470 0.29 +0.2 850,400 - MMP_RS04470 0.29 +0.0 850,401 + MMP_RS04470 0.29 -0.3 850,408 - MMP_RS04470 0.30 -0.0 850,408 - MMP_RS04470 0.30 +0.2 850,408 - MMP_RS04470 0.30 +0.2 850,409 + MMP_RS04470 0.30 +0.4 850,409 + MMP_RS04470 0.30 +0.5 850,480 - MMP_RS04470 0.35 -0.1 850,481 + MMP_RS04470 0.35 +0.3 850,553 + MMP_RS04470 0.40 +0.1 850,562 + MMP_RS04470 0.41 +0.4 850,586 - MMP_RS04470 0.43 -0.2 850,587 + MMP_RS04470 0.43 -0.1 850,587 + MMP_RS04470 0.43 +0.1 850,637 - MMP_RS04470 0.46 +0.7 850,805 - MMP_RS04470 0.59 +0.0 850,838 + MMP_RS04470 0.61 +0.1 850,841 - MMP_RS04470 0.61 -0.2 850,841 - MMP_RS04470 0.61 -0.2 850,934 + MMP_RS04470 0.68 -0.4 851,032 - MMP_RS04470 0.75 +0.1 851,037 + MMP_RS04470 0.75 -0.1 851,052 + MMP_RS04470 0.76 +0.1 851,066 + MMP_RS04470 0.77 -0.1 851,126 + MMP_RS04470 0.82 +0.3 851,180 - MMP_RS04470 0.86 +0.5 851,199 - MMP_RS04470 0.87 +0.2 851,205 + MMP_RS04470 0.88 +0.3 851,239 + +0.7 851,240 - +0.1 851,241 + -0.4 851,260 - +0.1 851,261 + -0.5 851,264 - -0.1 851,264 - +0.1 851,264 - -0.3 851,267 - +0.0 851,268 + -0.4 851,268 + +0.1 851,269 - -0.7 851,269 - +0.4 851,276 - +0.2 851,293 + +0.5 851,382 + MMP_RS04475 0.13 +0.2 851,382 + MMP_RS04475 0.13 +0.3 851,444 - MMP_RS04475 0.32 -0.3 851,484 - MMP_RS04475 0.45 -0.0 851,486 - MMP_RS04475 0.45 -0.3 851,513 + MMP_RS04475 0.54 -0.1 851,934 - +0.0 851,944 - +0.2 851,945 + +0.3 851,957 + +0.3 851,966 - -0.1 851,971 - +0.6 851,972 + -0.0 851,975 - -0.7 851,976 + -0.3 851,976 + +0.4 851,976 + +0.1 851,998 - +0.2 852,002 - -0.3 852,041 - +0.1 852,046 - -0.2 852,051 - +0.2 852,051 - -0.0 852,051 - +0.4 852,051 - +0.3 852,054 + -0.3 852,056 - -0.3 852,057 + -0.5 852,057 + +0.3 852,074 + -0.1 852,102 - +0.2 852,102 - +0.2 852,103 + -0.2 852,105 + +0.3 852,179 - +0.2 852,180 + -0.1 852,247 - +0.4 852,248 + +0.4 852,260 - +0.2 852,265 - +0.3 852,319 + +0.2 852,421 - MMP_RS04480 0.14 +0.5 852,422 + MMP_RS04480 0.14 +0.3 852,428 + MMP_RS04480 0.15 +0.2 852,428 + MMP_RS04480 0.15 +0.6 852,443 - MMP_RS04480 0.16 +0.4 852,451 + MMP_RS04480 0.16 -0.0 852,453 - MMP_RS04480 0.17 +0.3 852,454 + MMP_RS04480 0.17 +0.0 852,454 + MMP_RS04480 0.17 +0.5 852,454 + MMP_RS04480 0.17 +0.4 852,463 - MMP_RS04480 0.17 +0.3 852,500 - MMP_RS04480 0.20 +0.1 852,533 + MMP_RS04480 0.23 +0.4 852,533 + MMP_RS04480 0.23 +0.3 852,622 - MMP_RS04480 0.30 +0.1 852,627 - MMP_RS04480 0.30 +0.3 852,632 + MMP_RS04480 0.30 +0.3
Or see this region's nucleotide sequence