Experiment: Formate McCas no stress control 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02185 and MMP_RS02190 are separated by 70 nucleotides MMP_RS02190 and MMP_RS02195 are separated by 34 nucleotides MMP_RS02195 and MMP_RS02200 are separated by 150 nucleotides
MMP_RS02185: MMP_RS02185 - ribose-phosphate diphosphokinase, at 408,933 to 409,796
_RS02185
MMP_RS02190: MMP_RS02190 - sulfopyruvate decarboxylase subunit alpha, at 409,867 to 410,370
_RS02190
MMP_RS02195: MMP_RS02195 - tRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-methylthiotransferase, at 410,405 to 411,682
_RS02195
MMP_RS02200: MMP_RS02200 - methyl-accepting chemotaxis protein, at 411,833 to 414,028
_RS02200
Position (kb) 410
411
412 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 409.769 kb on + strand at 409.798 kb on - strand at 409.798 kb on - strand at 409.903 kb on - strand at 409.930 kb on + strand, within MMP_RS02190 at 410.106 kb on - strand, within MMP_RS02190 at 410.274 kb on + strand, within MMP_RS02190 at 410.349 kb on + strand at 410.371 kb on + strand at 410.527 kb on + strand at 410.622 kb on - strand, within MMP_RS02195 at 410.845 kb on - strand, within MMP_RS02195 at 410.872 kb on - strand, within MMP_RS02195 at 410.884 kb on + strand, within MMP_RS02195 at 411.366 kb on - strand, within MMP_RS02195 at 411.484 kb on + strand, within MMP_RS02195 at 411.540 kb on - strand, within MMP_RS02195 at 411.560 kb on + strand at 411.580 kb on - strand at 411.581 kb on + strand at 411.710 kb on - strand at 411.743 kb on - strand at 411.743 kb on - strand at 411.744 kb on + strand at 411.782 kb on - strand at 411.783 kb on + strand at 411.784 kb on - strand at 411.784 kb on - strand at 411.784 kb on - strand at 411.784 kb on - strand at 411.784 kb on - strand at 411.785 kb on + strand at 411.785 kb on + strand at 411.786 kb on - strand at 411.786 kb on - strand at 411.786 kb on - strand at 411.786 kb on - strand at 411.786 kb on - strand at 411.786 kb on - strand at 411.787 kb on + strand at 411.787 kb on + strand at 411.787 kb on + strand at 411.787 kb on + strand at 411.806 kb on + strand at 411.817 kb on - strand at 411.818 kb on + strand at 411.819 kb on - strand at 411.819 kb on - strand at 411.819 kb on - strand at 411.895 kb on + strand at 411.928 kb on - strand at 411.931 kb on - strand at 411.938 kb on + strand at 412.053 kb on + strand, within MMP_RS02200 at 412.093 kb on + strand, within MMP_RS02200 at 412.201 kb on - strand, within MMP_RS02200 at 412.202 kb on + strand, within MMP_RS02200 at 412.291 kb on - strand, within MMP_RS02200 at 412.349 kb on + strand, within MMP_RS02200 at 412.540 kb on + strand, within MMP_RS02200 at 412.616 kb on - strand, within MMP_RS02200 at 412.617 kb on + strand, within MMP_RS02200 at 412.617 kb on + strand, within MMP_RS02200 at 412.645 kb on - strand, within MMP_RS02200
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 1 remove 409,769 + -1.9 409,798 - -0.7 409,798 - -1.7 409,903 - -2.2 409,930 + MMP_RS02190 0.12 -1.6 410,106 - MMP_RS02190 0.47 -1.8 410,274 + MMP_RS02190 0.81 -0.1 410,349 + -0.3 410,371 + -0.5 410,527 + -0.1 410,622 - MMP_RS02195 0.17 -1.4 410,845 - MMP_RS02195 0.34 -2.3 410,872 - MMP_RS02195 0.37 -1.8 410,884 + MMP_RS02195 0.37 -1.9 411,366 - MMP_RS02195 0.75 -2.6 411,484 + MMP_RS02195 0.84 -3.0 411,540 - MMP_RS02195 0.89 -1.4 411,560 + -2.2 411,580 - -1.6 411,581 + -1.4 411,710 - +0.3 411,743 - +0.1 411,743 - -0.2 411,744 + -0.1 411,782 - -0.2 411,783 + -0.2 411,784 - +0.2 411,784 - +0.2 411,784 - -0.4 411,784 - +0.1 411,784 - +1.3 411,785 + +0.6 411,785 + +0.0 411,786 - -0.4 411,786 - -0.3 411,786 - -0.2 411,786 - -0.1 411,786 - -0.6 411,786 - +0.1 411,787 + +0.3 411,787 + -0.4 411,787 + +0.1 411,787 + +0.2 411,806 + +0.2 411,817 - -0.9 411,818 + +0.1 411,819 - -0.3 411,819 - -0.1 411,819 - +0.2 411,895 + +0.8 411,928 - +0.8 411,931 - -0.9 411,938 + +0.2 412,053 + MMP_RS02200 0.10 -0.3 412,093 + MMP_RS02200 0.12 +0.2 412,201 - MMP_RS02200 0.17 -0.2 412,202 + MMP_RS02200 0.17 +0.0 412,291 - MMP_RS02200 0.21 -0.1 412,349 + MMP_RS02200 0.23 -0.2 412,540 + MMP_RS02200 0.32 -0.6 412,616 - MMP_RS02200 0.36 -0.1 412,617 + MMP_RS02200 0.36 +0.2 412,617 + MMP_RS02200 0.36 -0.2 412,645 - MMP_RS02200 0.37 -0.2
Or see this region's nucleotide sequence